LOCUS       40924_21873        5147 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Wheat transformation vector pghp8.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Gil-Humanes J, Piston F, Tollefsen S, Sollid LM, Barro F.
  TITLE     Effective shutdown in the expression of celiac disease-related wheat
            gliadin T-cell epitopes by RNA interference
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 107 (39), 17023-17028 (2010)
  PUBMED    20829492
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Gil-Humanes J, Piston F, Tollefsen S, Aouni R, Sollid LM, Barro F.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) Mejora Genetica Vegetal, Instituto de 
            Agricultura Sostenible. CSIC, Alameda del Obispo. s/n, Cordoba, 
            Cordoba 14080, Spain
REFERENCE   3  (bases 1 to 5147)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5147)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2010"; volume: "107"; issue: "39"; pages:
            "17023-17028"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2010) Mejora Genetica Vegetal, Instituto de Agricultura 
            Sostenible. CSIC, Alameda del Obispo. s/n, Cordoba, Cordoba 14080, 
            Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      402..1237
                     /label=D-hordein promoter
                     /note="D-hordein promoter"
                     /regulatory_class="promoter"
     misc_feature    1244..1413
                     /note="pg8.1 sense; gamma-gliadin antisense"
     intron          1414..2437
                     /note="maize ubiquitin intron 1; Ubi 1 intron"
     misc_feature    2444..2612
                     /note="pg8.1 antisense; gamma-gliadin antisense"
     terminator      2635..2887
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2926..2942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2950..2966)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2974..3004)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3019..3040)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3328..3916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4090..4947)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4948..5052)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cccattaatt gaactcattc
      421 gggaagcggg aaaatttcca attctggtat aaatcaaact atttgacgcg aattttctct
      481 gaagatcata tgttaatttt agacatcacy gaccaaaggt ttcagttggt tgagttttgt
      541 cacggataca agatgcytcc atacgtcaaa aaattctacc aacttttggt acggtgcctc
      601 gtagcacgga tagatcttgt gtgtcactgg atagatgttg tgtgtcacta gattgatatt
      661 gtgagtcata gcatggattt gtgttgcctg gaaagggaay tacatgacaa gcaacaaaac
      721 ctgaaatgag cttttggaaa gatgatttat cagtttactt gttccatgca agctaccttc
      781 cactactcga catgcttaga agcttcgagt gcccgcggat ttgccaaagc aatggctaac
      841 agacrcatat tctgccaaaa acccagaacg ataatcrctt ctcgtagatg aagagaacag
      901 accaagatac aaacgtccac acttctgcaa acagtacccc agaactagga ttaagccgat
      961 tacgtggctt tagcagaccg tccaaaaaaa ctgctttgca aagctccaat tcctccttgc
     1021 ttatccaatt tcttttgtgt tggcaaactg cactttttcc aaccgattct gttcttcccg
     1081 tgtttcttct taggctagct aacatagccg tgcacacagc catggtccgg aaccttcacc
     1141 tcgtccctat aaaagcccag ccaatctcca caatctcttc atcaccgaga acaccgagca
     1201 ccacgaaact agagatcaat tcattgacag tcggatgggt acccatcatc atgcagcagc
     1261 agcagcaaca acaacaacaa caaggcatgc atatctttct gccactatct cagcagcaac
     1321 aggtgggtca aggttctcta gtccaaggcc agggcatcat ccaaccacaa caaccagctc
     1381 aattggaggc gatcagatca ttggtgttgc aatctagaca cctccgcttc aaggtacgcc
     1441 gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt
     1501 agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc
     1561 gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac
     1621 ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc
     1681 gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc
     1741 aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg
     1801 tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta
     1861 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     1921 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     1981 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     2041 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     2101 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     2161 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     2221 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     2281 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     2341 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     2401 cttttgtcga tgctcaccct gttgtttggt gttacttctg cagttgcaac accaatgatc
     2461 tgatcgcctc caattgagct ggttgttgtg gttggatgat gccctggcct tggactagag
     2521 aaccttgacc cacctgttgc tgctgagata gtggcagaaa gatatgcatg ccttgttgtt
     2581 gttgttgttg ctgctgctgc tgcatgatga tggtcgacct gcaggcatgc tcgagatcgt
     2641 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2701 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2761 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2821 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2881 ctagatccga tgataagctg tcraacaaag cttggcgtaa tcatggtcat agctgtttcc
     2941 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3001 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3061 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5041 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5101 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
