LOCUS       Exported               11561 bp DNA     circular SYN 15-JUL-2025
DEFINITION  Exported.
ACCESSION   V005900
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11561)
  AUTHORS   Lu R, Malcuit I, Moffett P, Ruiz MT, Peart J, Wu AJ, Rathjen JP, 
            Bendahmane A, Day L, Baulcombe DC.
  TITLE     High throughput virus-induced gene silencing implicates heat shock 
            protein 90 in plant disease resistance
  JOURNAL   EMBO J. 22 (21), 5690-5699 (2003)
  PUBMED    14592968
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   Lu R, Malcuit I, Moffett P, Ruiz T, Peart J, Wu A-J., Rathjen J, 
            Bendahmane A, Day L, Baulcombe D.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) Department of Plant Pathology, University of
            California Riverside, 900 University Avenue, Riverside, CA 92521, 
            USA
REFERENCE   3  (bases 1 to 11561)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11561)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11561)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003"; volume: "22"; issue: "21"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2003) Department of Plant Pathology, University of 
            California Riverside, 900 University Avenue, Riverside, CA 92521, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          10552..11327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1082..1425
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1510..5877
                     /codon_start=1
                     /label=RNA replication protein
                     /note="RNA replication protein from Potato virus X (strain
                     X3). Accession#: P17779"
                     /translation="MAKVREVYQSFTDSTTKTLIQDEAYRNIRPIMEKHKLANPYAQTV
                     EAANDLEGFGIATNPYSIELHTHAAAKTIENKLLEVLGSILPQEPVTFMFLKPRKLNYM
                     RRNPRIKDIFQNVAIEPRDVARYPKETIIDKLTEITTETAYISDTLHFLDPSYIVETFQ
                     NCPKLQTLYATLVLPVEAAFKMESTHPNIYSLKYFGDGFQYIPGNHGGGAYHHEFAHLQ
                     WLKVGKIKWRDPKDSFLGHLNYTTEQVEMHTVTVQLQESFAANHLYCIRRGDLLTPEVR
                     TFGQPDRYVIPPQIFLPKVHNCKKPILKKTMMQLFLYVRTVKVAKNCDIFAKVRQLIKS
                     SDLDKYSAVELVYLVSYMEFLADLQATTCFSDTLSGGLLTKTLAPVRAWIQEKKMQLFG
                     LEDYAKLVKAVDFHPVDFSFKVETWDFRFHPLQAWKAFRPREVSDVEEMESLFSDGDLL
                     DCFTRMPAYAVNAEEDLAAIRKTPEMDVGQEVKEPAGDRNQYSNPAETFLNKLHRKHSR
                     EVKHQAAKKAKRLAEIQESMRAEGDAEPNEISGGMGAIPSNAELPGTNDARQELTLPTT
                     KPVPARWEDASFTDSSVEEEQVKPLGKETVETATQQVIEGLPWKHWIPQLNAVGFKALE
                     IQRDRSGTMIMPITEMVSGLEKEDFPEGTPKELARELFAMNRSPATIPLDLLRARDYGS
                     DVKNKRIGAITKTQATSWGEYLTGKIESLTERKVATCVIHGAGGSGKSHAIQKALREIG
                     KGSDITVVLPTNELRLDWSKKVPNTEPYMFKTYEKALIGGTGSIVIFDDYSKLPPGYIE
                     ALVCFYSKIKLIILTGDSRQSVYHETAEDASIRHLGPATEYFSKYCRYYLNATHRNKKD
                     LANMLGVYSERTGVTEISMSAEFLEGIPTLVPSDEKRKLYMGTGRNDTFTYAGCQGLTK
                     PKVQIVLDHNTQVCSANVMYTALSRATDRIHFVNTSANSSAFWEKLDSTPYLKTFLSVV
                     REQALREYEPAEAEPIQEPEPQTHMCVENEESVLEEYKEELLEKFDREIHSESHGHSNC
                     VQTEDTTIQLFSHQQAKDETLLWATIDARLKTSNQETNFREFLSKKDIGDVLFLNYQKA
                     MGLPKERIPFSQEVWEACAHEVQSKYLSKSKCNLINGTVRQSPDFDENKIMVFLKSQWV
                     TKVEKLGLPKIKPGQTIAAFYQQTVMLFGTMARYMRWFRQAFQPKEVFINCETTPDDMS
                     AWALNNWNFSRPSLANDYTAFDQSQDGAMLQFEVLKAKHHCIPEEIIQAYIDIKTNAQI
                     FLGTLSIMRLTGEGPTFDANTECNIAYTHTKFDIPAGTAQVYAGDDSALDCVPEVKHSF
                     HRLEDKLLLKSKPVITQQKKGSWPEFCGWLITPKGVMKDPIKLHVSLKLAEAKGELKKC
                     QDSYEIDLSYAYDHKDSLHDLFDEKQCQAHTLTCRTLIKSGRGTVSLSRLRNFL"
     CDS             5911..6585
                     /codon_start=1
                     /label=Movement and silencing protein TGBp1
                     /note="Movement and silencing protein TGBp1 from Potato
                     virus X (strain X3). Accession#: P17780"
                     /translation="MDILISSLKSLGYSRTSKSLDSGPLVVHAVAGAGKSTALRKLILR
                     HPTFTVHTLGVPDKVSIRTRGIQKPGPIPEGNFAILDEYTLDNTTRNSYQALFADPYQA
                     PEFSLEPHFYLETSFRVPRKVADLIAGCGFDFETNSPEEGHLEITGIFKGPLLGKVIAI
                     DEESETTLSRHGVEFVKPCQVTGLEFKVVTIVSAAPIEEIGQSTAFYNAITRSKGLTYV
                     RAG"
     misc_feature    7093..7110
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    7111..7988
                     /label=Potato virus X strain UK3 3'-sequence
                     /note="Potato virus X strain UK3 3'-sequence"
     terminator      8013..8265
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8269..8436
                     /label=shuttle vector pSK5640 sequence
                     /note="shuttle vector pSK5640 sequence"
     primer_bind     complement(8272..8288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8455..8473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8494..8510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8518..8534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8542..8572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8587..8608)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    8847..8871
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8962..9550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9654..10466)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tctcaaacaa
      541 acacatacag cgacttagtt tacccgccaa tatatcctgt caaggccttc atgttctttc
      601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      841 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      901 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      961 accctcacta aagggaacaa aagctggagc tcgcatgcct gcaggtcaac atggtggagc
     1021 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     1081 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     1141 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     1201 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     1261 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     1321 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     1381 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggagaaaa ctaaaccata
     1441 caccaccaac acaaccaaac ccaccacgcc caattgttac acacccgctt gaaaaagaaa
     1501 gtttaacaaa tggccaaggt gcgcgaggtt taccaatctt ttacagactc caccacaaaa
     1561 actctcatcc aagatgaggc ttatagaaac attcgcccca tcatggaaaa acacaaacta
     1621 gctaaccctt acgctcaaac ggttgaagcg gctaatgatc tagaggggtt cggcatagcc
     1681 accaatccct atagcattga attgcataca catgcagccg ctaagaccat agagaataaa
     1741 cttctagagg tgcttggttc catcctacca caagaacctg ttacatttat gtttcttaaa
     1801 cccagaaagc taaactacat gagaagaaac ccgcggatca aggacatttt ccaaaatgtt
     1861 gccattgaac caagagacgt agccaggtac cccaaggaaa caataattga caaactcaca
     1921 gagatcacaa cggaaacagc atacattagt gacactctgc acttcttgga tccgagctac
     1981 atagtggaga cattccaaaa ctgcccaaaa ttgcaaacat tgtatgcgac cttagttctc
     2041 cccgttgagg cagcctttaa aatggaaagc actcacccga acatatacag cctcaaatac
     2101 ttcggagatg gtttccagta tataccaggc aaccatggtg gcggggcata ccatcatgaa
     2161 ttcgctcatc tacaatggct caaagtggga aagatcaagt ggagggaccc caaggatagc
     2221 tttctcggac atctcaatta cacgactgag caggttgaga tgcacacagt gacagtacag
     2281 ttgcaggaat cgttcgcggc aaaccacttg tactgcatca ggagaggaga cttgctcaca
     2341 ccggaggtgc gcactttcgg ccaacctgac aggtacgtga ttccaccaca gatcttcctc
     2401 ccaaaagttc acaactgcaa gaagccgatt ctcaagaaaa ctatgatgca gctcttcttg
     2461 tatgttagga cagtcaaggt cgcaaaaaat tgtgacattt ttgccaaagt cagacaatta
     2521 attaaatcat ctgacttgga caaatactct gctgtggaac tggtttactt agtaagctac
     2581 atggagttcc ttgccgattt acaagctacc acctgcttct cagacacact ttctggtggc
     2641 ttgctaacaa agacccttgc accggtgagg gcttggatac aagagaaaaa gatgcagctg
     2701 tttggtcttg aggactacgc gaagttagtc aaagcagttg atttccaccc ggtggatttt
     2761 tctttcaaag tggaaacttg ggacttcaga ttccacccct tgcaagcgtg gaaagccttc
     2821 cgaccaaggg aagtgtcgga tgtagaggaa atggaaagtt tgttctcaga tggggacttg
     2881 cttgattgct tcacaagaat gccagcttat gcggtaaacg cagaggaaga tttagctgca
     2941 atcaggaaaa cgcccgagat ggatgtcggt caagaagtta aagaacctgc aggagacaga
     3001 aatcaatact caaaccctgc agaaactttc ctcaacaagc tccacaggaa acacagtagg
     3061 gaggtgaaac accaggccgc aaagaaagct aaacgcctag ctgaaatcca ggagtcaatg
     3121 agagctgaag gtgatgccga accaaatgaa ataagcgggg gcatgggggc aatacccagc
     3181 aacgccgaac ttcctggcac gaatgatgcc agacaagaac tcacactccc aaccactaaa
     3241 cctgtccctg caaggtggga agatgcttca ttcacagatt ctagtgtgga agaggagcag
     3301 gttaaacccc ttggaaaaga aaccgttgaa acagcgacgc aacaagtcat cgaaggactt
     3361 ccttggaaac actggattcc tcaattaaat gctgttggat tcaaggcgct ggaaattcag
     3421 agggatagga gtggaacaat gatcatgccc atcacagaaa tggtctccgg gctggaaaaa
     3481 gaggacttcc ctgaaggaac tccaaaagag ttggcacgag aattgttcgc tatgaacaga
     3541 agccctgcca ccatcccttt ggacctgctt agagccagag actacggcag tgatgtaaag
     3601 aacaagagaa ttggtgccat cacaaagaca caggcaacga gttggggcga atacttgaca
     3661 ggaaagatag aaagcttgac tgagaggaaa gttgcgactt gtgtcattca tggagctgga
     3721 ggttctggaa aaagtcatgc catccagaag gcattgagag aaattggcaa gggctcggac
     3781 atcactgtag tcctgccgac caatgaactg cggctagatt ggagtaagaa agtgcctaac
     3841 actgagccct atatgttcaa gacctatgaa aaggcgttaa ttgggggaac aggcagcata
     3901 gtcatctttg acgattactc aaaacttcct cccggttaca tagaagcctt agtctgtttc
     3961 tactctaaaa tcaagctaat cattctaaca ggagatagca gacaaagcgt ctaccatgaa
     4021 actgctgagg acgcctccat caggcatttg ggaccagcaa cagagtactt ctcaaaatac
     4081 tgccgatact atctcaatgc cacacaccgc aacaagaaag atcttgcgaa catgcttggt
     4141 gtctacagtg agagaacggg agtcaccgaa atcagcatga gcgccgagtt cttagaagga
     4201 atcccaactt tggtaccctc ggatgagaag agaaagctgt acatgggcac cgggaggaat
     4261 gacacgttca catacgctgg atgccagggg ctaactaagc cgaaggtaca aatagtgttg
     4321 gaccacaaca cccaagtgtg tagcgcgaat gtgatgtaca cggcactttc tagagccacc
     4381 gataggattc acttcgtgaa cacaagtgca aattcctcgg ccttctggga aaagttggac
     4441 agcacccctt acctcaagac tttcctatca gtggtgagag aacaagcact cagggagtac
     4501 gagccggcag aggcagagcc aattcaagag cctgagcccc agacacacat gtgtgtcgag
     4561 aatgaggagt ccgtgctaga agagtacaaa gaggaactct tggaaaagtt tgacagagag
     4621 atccactctg aatcccatgg tcattcaaac tgtgtccaaa ctgaagacac aaccattcag
     4681 ttgttttcgc atcaacaagc aaaagatgag accctcctct gggcgactat agatgcgcgg
     4741 ctcaagacca gcaatcaaga gacaaacttc cgagaattcc tgagcaagaa ggacattggg
     4801 gacgttctgt ttttaaacta ccaaaaagct atgggtttac ccaaagagcg tattcctttt
     4861 tcccaagagg tctgggaagc ttgtgcccac gaagtacaaa gcaagtacct cagcaagtca
     4921 aagtgcaact tgatcaatgg gactgtgaga cagagcccag acttcgatga aaataagatt
     4981 atggtattcc tcaagtcgca gtgggtcaca aaggtggaaa aactaggtct acccaagatt
     5041 aagccaggtc aaaccatagc agccttttac cagcagactg tgatgctttt tggaactatg
     5101 gctaggtaca tgcgatggtt cagacaggct ttccagccaa aagaagtctt cataaactgt
     5161 gagaccacgc cagatgacat gtctgcatgg gccttgaaca actggaattt cagcagacct
     5221 agcttggcta atgactacac agctttcgac cagtctcagg atggagccat gttgcaattt
     5281 gaggtgctca aagccaaaca ccactgcata ccagaggaaa tcattcaggc atacatagat
     5341 attaagacta atgcacagat tttcctaggc acgttatcaa ttatgcgcct gactggtgaa
     5401 ggtcccactt ttgatgcaaa cactgagtgc aacatagctt acacccatac aaagtttgac
     5461 atcccagccg gaactgctca agtttatgca ggagacgact ccgcactgga ctgtgttcca
     5521 gaagtgaagc atagtttcca caggcttgag gacaaattac tcctaaagtc aaagcctgta
     5581 atcacgcagc aaaagaaggg cagttggcct gagttttgtg gttggctgat cacaccaaaa
     5641 ggggtgatga aagacccaat taagctccat gttagcttaa aattggctga agctaagggt
     5701 gaactcaaga aatgtcaaga ttcctatgaa attgatctga gttatgccta tgaccacaag
     5761 gactctctgc atgacttgtt cgatgagaaa cagtgtcagg cacacacact cacttgcaga
     5821 acactaatca agtcagggag aggcactgtc tcactttccc gcctcagaaa ctttctttaa
     5881 ccgttaagtt accttagaga tttgaataag atggatattc tcatcagtag tttgaaaagt
     5941 ttaggttatt ctaggacttc caaatcttta gattcaggac ctttggtagt acatgcagta
     6001 gccggagccg gtaagtccac agccctaagg aagttgatcc tcagacaccc aacattcacc
     6061 gtgcatacac tcggtgtccc tgacaaggtg agtatcagaa ctagaggcat acagaagcca
     6121 ggacctattc ctgagggcaa cttcgcaatc ctcgatgagt atactttgga caacaccaca
     6181 aggaactcat accaggcact ttttgctgac ccttatcagg caccggagtt tagcctagag
     6241 ccccacttct acttggaaac atcatttcga gttccgagga aagtggcaga tttgatagct
     6301 ggctgtggct tcgatttcga gaccaactca ccggaagaag ggcacttaga gatcactggc
     6361 atattcaaag ggcccctact cggaaaggtg atagccattg atgaggagtc tgagacaaca
     6421 ctgtccaggc atggtgttga gtttgttaag ccctgccaag tgacgggact tgagttcaaa
     6481 gtagtcacta ttgtgtctgc cgcaccaata gaggaaattg gccagtccac agctttctac
     6541 aacgctatca ccaggtcaaa gggattgaca tatgtccgcg cagggccata ggctgaccgc
     6601 tccggtcaat tctgaaaaag tgtacatagt attaggtcta tcatttgctt tagtttcaat
     6661 tacctttctg ctttctagaa atagcttacc ccacgtcggt gacaacattc acagcttgcc
     6721 acacggagga gcttacagag acggcaccaa agcaatcttg tacaactccc caaatctagg
     6781 gtcacgagtg agtctacaca acggaaagaa cgcagcattt gctgccgttt tgctactgac
     6841 tttgctgatc tatggaagta aatacatatc tcaacgcaat catacttgtg cttgtggtaa
     6901 caatcatagc agtcattagc acttccttag tgaggactga accttgtgtc atcaagatta
     6961 ctggggaatc aatcacagtg ttggcttgca aactagatgc agaaaccata agggccattg
     7021 ccgatctcaa gccactctcc gttgaacggt taagtttcca ttgatactcg aaagaggtca
     7081 gcaccagcta gcatcgattc ccgggtcgac cgccgataag cttgataggg ccattgccga
     7141 tctcaagcca ctctccgttg aacggttaag tttccattga tactcgaaag atgtcagcac
     7201 cagctagcac aacacagccc atagggtcaa ctacctcaac taccacaaaa actgcaggcg
     7261 caactcctgc cacagcttca ggcctgttca ccatcccgga tggggatttc tttagtacag
     7321 cccgtgccat agtagccagc aatgctgtcg caacaaatga ggacctcagc aagattgagg
     7381 ctatttggaa ggacatgaag gtgcccacag acactatggc acaggctgct tgggacttag
     7441 tcagacactg tgctgatgta ggatcatccg ctcaaacaga aatgatagat acaggtccct
     7501 attccaacgg catcagcaga gctagactgg cagcagcaat taaagaggtg tgcacactta
     7561 ggcaattttg catgaagtat gctccagtgg tatggaactg gatgttaact aacaacagtc
     7621 cacctgctaa ctggcaagca caaggtttca agcctgagca caaattcgct gcattcgact
     7681 tcttcaatgg agtcaccaac ccagctgcca tcatgcccaa agaggggctc atccggccac
     7741 cgtctgaagc tgaaatgaat gctgcccaaa ctgctgcctt tgtgaagatt acaaaggcca
     7801 gggcacaatc caacgacttt gccagcctag atgcagctgt cactcgaggt cgtatcactg
     7861 gaacaacaac cgctgaggct gttgtcactc taccaccacc ataactacgt ctacataacc
     7921 gacgcctacc ccagtttcat agtattttct ggtttgattg tatgaataat ataaataaaa
     7981 aaaaaaaact agtgggtacc gcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     8041 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     8101 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     8161 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     8221 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgaatt cactggccgt
     8281 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8341 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8401 acagttgcgc agcctgaatg gcgaatggcg cctgatgagc tccagctttt gttcccttta
     8461 gtgagggtta attccgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8821 taacgcagga aagaacatga aggccttgac aggatatatt ggcgggtaaa ctaagtcgct
     8881 gtatgtgttt gtttgagatc tcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8941 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9001 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9061 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9121 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9181 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9241 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     9301 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     9361 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     9421 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9481 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9541 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9601 ctcacgttaa gggattttgg tcatggttac aaccaattaa ccaattctga ttagaaaaac
     9661 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     9721 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     9781 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     9841 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     9901 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     9961 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
    10021 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
    10081 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
    10141 acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta
    10201 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
    10261 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
    10321 gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga
    10381 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa
    10441 gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
    10501 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttgg
    10561 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
    10621 tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
    10681 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
    10741 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
    10801 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
    10861 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
    10921 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct
    10981 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac
    11041 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
    11101 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
    11161 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
    11221 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
    11281 ggcattatct gaacataaaa cactatcaat aagttggagt cattaccaga ttttgagaca
    11341 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc
    11401 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca
    11461 cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga
    11521 ttcaggcgat ccccatccaa cagcccgccg tcgagcgggc t
//
