LOCUS       Exported               11148 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Exported.
ACCESSION   V005901
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Lu R, Malcuit I, Moffett P, Ruiz MT, Peart J, Wu AJ, Rathjen JP, 
            Bendahmane A, Day L, Baulcombe DC.
  TITLE     High throughput virus-induced gene silencing implicates heat shock 
            protein 90 in plant disease resistance
  JOURNAL   EMBO J. 22 (21), 5690-5699 (2003)
  PUBMED    14592968
REFERENCE   2  (bases 1 to 11148)
  AUTHORS   Lu R, Malcuit I, Moffett P, Ruiz T, Peart J, Wu A-J., Rathjen J, 
            Bendahmane A, Day L, Baulcombe D.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) Department of Plant Pathology, University of
            California Riverside, 900 University Avenue, Riverside, CA 92521, 
            USA
REFERENCE   3  (bases 1 to 11148)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11148)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11148)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2003"; volume: "22"; issue: "21"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2003) Department of Plant Pathology, University of 
            California Riverside, 900 University Avenue, Riverside, CA 92521, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          10142..10917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    558..582
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    821..842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        857..887
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    895..911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     919..935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        956..974
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1082..1427
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1510..5877
                     /codon_start=1
                     /label=RNA replication protein
                     /note="RNA replication protein from Potato virus X (strain
                     X3). Accession#: P17779"
                     /translation="MAKVREVYQSFTDSTTKTLIQDEAYRNIRPIMEKHKLANPYAQTV
                     EAANDLEGFGIATNPYSIELHTHAAAKTIENKLLEVLGSILPQEPVTFMFLKPRKLNYM
                     RRNPRIKDIFQNVAIEPRDVARYPKETIIDKLTEITTETAYISDTLHFLDPSYIVETFQ
                     NCPKLQTLYATLVLPVEAAFKMESTHPNIYSLKYFGDGFQYIPGNHGGGAYHHEFAHLQ
                     WLKVGKIKWRDPKDSFLGHLNYTTEQVEMHTVTVQLQESFAANHLYCIRRGDLLTPEVR
                     TFGQPDRYVIPPQIFLPKVHNCKKPILKKTMMQLFLYVRTVKVAKNCDIFAKVRQLIKS
                     SDLDKYSAVELVYLVSYMEFLADLQATTCFSDTLSGGLLTKTLAPVRAWIQEKKMQLFG
                     LEDYAKLVKAVDFHPVDFSFKVETWDFRFHPLQAWKAFRPREVSDVEEMESLFSDGDLL
                     DCFTRMPAYAVNAEEDLAAIRKTPEMDVGQEVKEPAGDRNQYSNPAETFLNKLHRKHSR
                     EVKHQAAKKAKRLAEIQESMRAEGDAEPNEISGGMGAIPSNAELPGTNDARQELTLPTT
                     KPVPARWEDASFTDSSVEEEQVKPLGKETVETATQQVIEGLPWKHWIPQLNAVGFKALE
                     IQRDRSGTMIMPITEMVSGLEKEDFPEGTPKELARELFAMNRSPATIPLDLLRARDYGS
                     DVKNKRIGAITKTQATSWGEYLTGKIESLTERKVATCVIHGAGGSGKSHAIQKALREIG
                     KGSDITVVLPTNELRLDWSKKVPNTEPYMFKTYEKALIGGTGSIVIFDDYSKLPPGYIE
                     ALVCFYSKIKLIILTGDSRQSVYHETAEDASIRHLGPATEYFSKYCRYYLNATHRNKKD
                     LANMLGVYSERTGVTEISMSAEFLEGIPTLVPSDEKRKLYMGTGRNDTFTYAGCQGLTK
                     PKVQIVLDHNTQVCSANVMYTALSRATDRIHFVNTSANSSAFWEKLDSTPYLKTFLSVV
                     REQALREYEPAEAEPIQEPEPQTHMCVENEESVLEEYKEELLEKFDREIHSESHGHSNC
                     VQTEDTTIQLFSHQQAKDETLLWATIDARLKTSNQETNFREFLSKKDIGDVLFLNYQKA
                     MGLPKERIPFSQEVWEACAHEVQSKYLSKSKCNLINGTVRQSPDFDENKIMVFLKSQWV
                     TKVEKLGLPKIKPGQTIAAFYQQTVMLFGTMARYMRWFRQAFQPKEVFINCETTPDDMS
                     AWALNNWNFSRPSLANDYTAFDQSQDGAMLQFEVLKAKHHCIPEEIIQAYIDIKTNAQI
                     FLGTLSIMRLTGEGPTFDANTECNIAYTHTKFDIPAGTAQVYAGDDSALDCVPEVKHSF
                     HRLEDKLLLKSKPVITQQKKGSWPEFCGWLITPKGVMKDPIKLHVSLKLAEAKGELKKC
                     QDSYEIDLSYAYDHKDSLHDLFDEKQCQAHTLTCRTLIKSGRGTVSLSRLRNFL"
     CDS             5911..6585
                     /codon_start=1
                     /label=Movement and silencing protein TGBp1
                     /note="Movement and silencing protein TGBp1 from Potato
                     virus X (strain X3). Accession#: P17780"
                     /translation="MDILISSLKSLGYSRTSKSLDSGPLVVHAVAGAGKSTALRKLILR
                     HPTFTVHTLGVPDKVSIRTRGIQKPGPIPEGNFAILDEYTLDNTTRNSYQALFADPYQA
                     PEFSLEPHFYLETSFRVPRKVADLIAGCGFDFETNSPEEGHLEITGIFKGPLLGKVIAI
                     DEESETTLSRHGVEFVKPCQVTGLEFKVVTIVSAAPIEEIGQSTAFYNAITRSKGLTYV
                     RAG"
     misc_feature    7093..7125
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    7126..8002
                     /label=Potato virus X strain UK3 3'-sequence
                     /note="Potato virus X strain UK3 3'-sequence"
     terminator      8027..8279
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8285..8452
                     /label=shuttle vector pSK5640 sequence
                     /note="shuttle vector pSK5640 sequence"
     misc_feature    8460..8482
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      complement(8549..9137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9238..10053)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     mobile_element  10147..10914
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(11120..11148,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tctcaaacaa
      541 acacatacag cgacttagtt tacccgccaa tatatcctgt caaggccttc atgttctttc
      601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      841 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      901 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      961 accctcacta aagggaacaa aagctggagc tcgcatgcct gcaggtcaac atggtggagc
     1021 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     1081 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     1141 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     1201 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     1261 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     1321 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
     1381 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggagaaaa ctaaaccata
     1441 caccaccaac acaaccaaac ccaccacgcc caattgttac acacccgctt gaaaaagaaa
     1501 gtttaacaaa tggccaaggt gcgcgaggtt taccaatctt ttacagactc caccacaaaa
     1561 actctcatcc aagatgaggc ttatagaaac attcgcccca tcatggaaaa acacaaacta
     1621 gctaaccctt acgctcaaac ggttgaagcg gctaatgatc tagaggggtt cggcatagcc
     1681 accaatccct atagcattga attgcataca catgcagccg ctaagaccat agagaataaa
     1741 cttctagagg tgcttggttc catcctacca caagaacctg ttacatttat gtttcttaaa
     1801 cccagaaagc taaactacat gagaagaaac ccgcggatca aggacatttt ccaaaatgtt
     1861 gccattgaac caagagacgt agccaggtac cccaaggaaa caataattga caaactcaca
     1921 gagatcacaa cggaaacagc atacattagt gacactctgc acttcttgga tccgagctac
     1981 atagtggaga cattccaaaa ctgcccaaaa ttgcaaacat tgtatgcgac cttagttctc
     2041 cccgttgagg cagcctttaa aatggaaagc actcacccga acatatacag cctcaaatac
     2101 ttcggagatg gtttccagta tataccaggc aaccatggtg gcggggcata ccatcatgaa
     2161 ttcgctcatc tacaatggct caaagtggga aagatcaagt ggagggaccc caaggatagc
     2221 tttctcggac atctcaatta cacgactgag caggttgaga tgcacacagt gacagtacag
     2281 ttgcaggaat cgttcgcggc aaaccacttg tactgcatca ggagaggaga cttgctcaca
     2341 ccggaggtgc gcactttcgg ccaacctgac aggtacgtga ttccaccaca gatcttcctc
     2401 ccaaaagttc acaactgcaa gaagccgatt ctcaagaaaa ctatgatgca gctcttcttg
     2461 tatgttagga cagtcaaggt cgcaaaaaat tgtgacattt ttgccaaagt cagacaatta
     2521 attaaatcat ctgacttgga caaatactct gctgtggaac tggtttactt agtaagctac
     2581 atggagttcc ttgccgattt acaagctacc acctgcttct cagacacact ttctggtggc
     2641 ttgctaacaa agacccttgc accggtgagg gcttggatac aagagaaaaa gatgcagctg
     2701 tttggtcttg aggactacgc gaagttagtc aaagcagttg atttccaccc ggtggatttt
     2761 tctttcaaag tggaaacttg ggacttcaga ttccacccct tgcaagcgtg gaaagccttc
     2821 cgaccaaggg aagtgtcgga tgtagaggaa atggaaagtt tgttctcaga tggggacttg
     2881 cttgattgct tcacaagaat gccagcttat gcggtaaacg cagaggaaga tttagctgca
     2941 atcaggaaaa cgcccgagat ggatgtcggt caagaagtta aagaacctgc aggagacaga
     3001 aatcaatact caaaccctgc agaaactttc ctcaacaagc tccacaggaa acacagtagg
     3061 gaggtgaaac accaggccgc aaagaaagct aaacgcctag ctgaaatcca ggagtcaatg
     3121 agagctgaag gtgatgccga accaaatgaa ataagcgggg gcatgggggc aatacccagc
     3181 aacgccgaac ttcctggcac gaatgatgcc agacaagaac tcacactccc aaccactaaa
     3241 cctgtccctg caaggtggga agatgcttca ttcacagatt ctagtgtgga agaggagcag
     3301 gttaaacccc ttggaaaaga aaccgttgaa acagcgacgc aacaagtcat cgaaggactt
     3361 ccttggaaac actggattcc tcaattaaat gctgttggat tcaaggcgct ggaaattcag
     3421 agggatagga gtggaacaat gatcatgccc atcacagaaa tggtctccgg gctggaaaaa
     3481 gaggacttcc ctgaaggaac tccaaaagag ttggcacgag aattgttcgc tatgaacaga
     3541 agccctgcca ccatcccttt ggacctgctt agagccagag actacggcag tgatgtaaag
     3601 aacaagagaa ttggtgccat cacaaagaca caggcaacga gttggggcga atacttgaca
     3661 ggaaagatag aaagcttgac tgagaggaaa gttgcgactt gtgtcattca tggagctgga
     3721 ggttctggaa aaagtcatgc catccagaag gcattgagag aaattggcaa gggctcggac
     3781 atcactgtag tcctgccgac caatgaactg cggctagatt ggagtaagaa agtgcctaac
     3841 actgagccct atatgttcaa gacctatgaa aaggcgttaa ttgggggaac aggcagcata
     3901 gtcatctttg acgattactc aaaacttcct cccggttaca tagaagcctt agtctgtttc
     3961 tactctaaaa tcaagctaat cattctaaca ggagatagca gacaaagcgt ctaccatgaa
     4021 actgctgagg acgcctccat caggcatttg ggaccagcaa cagagtactt ctcaaaatac
     4081 tgccgatact atctcaatgc cacacaccgc aacaagaaag atcttgcgaa catgcttggt
     4141 gtctacagtg agagaacggg agtcaccgaa atcagcatga gcgccgagtt cttagaagga
     4201 atcccaactt tggtaccctc ggatgagaag agaaagctgt acatgggcac cgggaggaat
     4261 gacacgttca catacgctgg atgccagggg ctaactaagc cgaaggtaca aatagtgttg
     4321 gaccacaaca cccaagtgtg tagcgcgaat gtgatgtaca cggcactttc tagagccacc
     4381 gataggattc acttcgtgaa cacaagtgca aattcctcgg ccttctggga aaagttggac
     4441 agcacccctt acctcaagac tttcctatca gtggtgagag aacaagcact cagggagtac
     4501 gagccggcag aggcagagcc aattcaagag cctgagcccc agacacacat gtgtgtcgag
     4561 aatgaggagt ccgtgctaga agagtacaaa gaggaactct tggaaaagtt tgacagagag
     4621 atccactctg aatcccatgg tcattcaaac tgtgtccaaa ctgaagacac aaccattcag
     4681 ttgttttcgc atcaacaagc aaaagatgag accctcctct gggcgactat agatgcgcgg
     4741 ctcaagacca gcaatcaaga gacaaacttc cgagaattcc tgagcaagaa ggacattggg
     4801 gacgttctgt ttttaaacta ccaaaaagct atgggtttac ccaaagagcg tattcctttt
     4861 tcccaagagg tctgggaagc ttgtgcccac gaagtacaaa gcaagtacct cagcaagtca
     4921 aagtgcaact tgatcaatgg gactgtgaga cagagcccag acttcgatga aaataagatt
     4981 atggtattcc tcaagtcgca gtgggtcaca aaggtggaaa aactaggtct acccaagatt
     5041 aagccaggtc aaaccatagc agccttttac cagcagactg tgatgctttt tggaactatg
     5101 gctaggtaca tgcgatggtt cagacaggct ttccagccaa aagaagtctt cataaactgt
     5161 gagaccacgc cagatgacat gtctgcatgg gccttgaaca actggaattt cagcagacct
     5221 agcttggcta atgactacac agctttcgac cagtctcagg atggagccat gttgcaattt
     5281 gaggtgctca aagccaaaca ccactgcata ccagaggaaa tcattcaggc atacatagat
     5341 attaagacta atgcacagat tttcctaggc acgttatcaa ttatgcgcct gactggtgaa
     5401 ggtcccactt ttgatgcaaa cactgagtgc aacatagctt acacccatac aaagtttgac
     5461 atcccagccg gaactgctca agtttatgca ggagacgact ccgcactgga ctgtgttcca
     5521 gaagtgaagc atagtttcca caggcttgag gacaaattac tcctaaagtc aaagcctgta
     5581 atcacgcagc aaaagaaggg cagttggcct gagttttgtg gttggctgat cacaccaaaa
     5641 ggggtgatga aagacccaat taagctccat gttagcttaa aattggctga agctaagggt
     5701 gaactcaaga aatgtcaaga ttcctatgaa attgatctga gttatgccta tgaccacaag
     5761 gactctctgc atgacttgtt cgatgagaaa cagtgtcagg cacacacact cacttgcaga
     5821 acactaatca agtcagggag aggcactgtc tcactttccc gcctcagaaa ctttctttaa
     5881 ccgttaagtt accttagaga tttgaataag atggatattc tcatcagtag tttgaaaagt
     5941 ttaggttatt ctaggacttc caaatcttta gattcaggac ctttggtagt acatgcagta
     6001 gccggagccg gtaagtccac agccctaagg aagttgatcc tcagacaccc aacattcacc
     6061 gtgcatacac tcggtgtccc tgacaaggtg agtatcagaa ctagaggcat acagaagcca
     6121 ggacctattc ctgagggcaa cttcgcaatc ctcgatgagt atactttgga caacaccaca
     6181 aggaactcat accaggcact ttttgctgac ccttatcagg caccggagtt tagcctagag
     6241 ccccacttct acttggaaac atcatttcga gttccgagga aagtggcaga tttgatagct
     6301 ggctgtggct tcgatttcga gaccaactca ccggaagaag ggcacttaga gatcactggc
     6361 atattcaaag ggcccctact cggaaaggtg atagccattg atgaggagtc tgagacaaca
     6421 ctgtccaggc atggtgttga gtttgttaag ccctgccaag tgacgggact tgagttcaaa
     6481 gtagtcacta ttgtgtctgc cgcaccaata gaggaaattg gccagtccac agctttctac
     6541 aacgctatca ccaggtcaaa gggattgaca tatgtccgcg cagggccata ggctgaccgc
     6601 tccggtcaat tctgaaaaag tgtacatagt attaggtcta tcatttgctt tagtttcaat
     6661 tacctttctg ctttctagaa atagcttacc ccacgtcggt gacaacattc acagcttgcc
     6721 acacggagga gcttacagag acggcaccaa agcaatcttg tacaactccc caaatctagg
     6781 gtcacgagtg agtctacaca acggaaagaa cgcagcattt gctgccgttt tgctactgac
     6841 tttgctgatc tatggaagta aatacatatc tcaacgcaat catacttgtg cttgtggtaa
     6901 caatcatagc agtcattagc acttccttag tgaggactga accttgtgtc atcaagatta
     6961 ctggggaatc aatcacagtg ttggcttgca aactagatgc agaaaccata agggccattg
     7021 ccgatctcaa gccactctcc gttgaacggt taagtttcca ttgatactcg aaagaggtca
     7081 gcaccagcta gcatcgattg gcgcgcctgg agcggccgcg tcgaccgccg ataagcttga
     7141 tagggccatt gccgatctca agccactctc cgttgaacgg ttaagtttcc attgatactc
     7201 gaaagatgtc agcaccagct agcacaacac agcccatagg gtcaactacc tcaactacca
     7261 caaaaactgc aggcgcaact cctgccacag cttcaggcct gttcaccatc ccggatgggg
     7321 atttctttag tacagcccgt gccatagtag ccagcaatgc tgtcgcaaca aatgaggacc
     7381 tcagcaagat tgaggctatt tggaaggaca tgaaggtgcc cacagacact atggcacagg
     7441 ctgcttggga cttagtcaga cactgtgctg atgtaggatc atccgctcaa acagaaatga
     7501 tagatacagg tccctattcc aacggcatca gcagagctag actggcagca gcaattaaag
     7561 aggtgtgcac acttaggcaa ttttgcatga agtatgctcc agtggtatgg aactggatgt
     7621 taactaacaa cagtccacct gctaactggc aagcacaagg tttcaagcct gagcacaaat
     7681 tcgctgcatt cgacttcttc aatggagtca ccaacccagc tgccatcatg cccaaagagg
     7741 ggctcatccg gccaccgtct gaagctgaaa tgaatgctgc ccaaactgct gcctttgtga
     7801 agattacaaa ggccagggca caatccaacg actttgccag cctagatgca gctgtcactc
     7861 gaggtcgtat cactggaaca acaaccgctg aggctgttgt cactctacca ccaccataac
     7921 tacgtctaca taaccgacgc ctaccccagt ttcatagtat tttctggttt gattgtatga
     7981 ataatataaa taaaaaaaaa aactagtggg taccgcgaat ttccccgatc gttcaaacat
     8041 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
     8101 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
     8161 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
     8221 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
     8281 ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8341 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8401 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgagaacgt
     8461 tacaccacaa tatatcctgc caagatctca tgtgagcaaa aggccagcaa aaggccagga
     8521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     9001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     9061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     9121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     9181 acgaaaactc acgttaaggg attttggtca tggttacaac caattaacca attctgatta
     9241 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     9301 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     9361 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     9421 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     9481 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     9541 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     9601 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     9661 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     9721 ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc
     9781 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     9841 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     9901 ctctggcgca tcgggcttcc catacaatcg gtagattgtc gcacctgatt gcccgacatt
     9961 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
    10021 tgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
    10081 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
    10141 attttgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
    10201 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
    10261 cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca
    10321 actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc
    10381 catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat
    10441 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt
    10501 gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc
    10561 cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc
    10621 ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg
    10681 gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac
    10741 gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac
    10801 ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa
    10861 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccagattt
    10921 tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc
    10981 acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac
    11041 tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat
    11101 ggggcgattc aggcgatccc catccaacag cccgccgtcg agcgggct
//
