LOCUS       Exported               12872 bp DNA     circular SYN 03-NOV-2025
DEFINITION  Exported.
ACCESSION   V000058
VERSION     .
KEYWORDS    pfNL43-dE-EGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12872)
  TITLE     NLdDIS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12872)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12872)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             69..326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    373..498
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     CDS             482..1984
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             1777..4788
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4473..4590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4733..5308
                     /gene="vif"
                     /label=Virion infectivity factor
                     /note="Virion infectivity factor from Human
                     immunodeficiency virus type 1 group M subtype B (isolate 
                     NY5). Accession#: P12504"
     CDS             5522..5695
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
     CDS             5753..5995
                     /gene="vpu"
                     /label=Protein Vpu
                     /note="Protein Vpu from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH10). Accession#: P69699"
     regulatory      6045..6054
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6051..6767
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    7289..7522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7707..7751
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             8045..8071
                     /label=NES
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             8317..8343
                     /gene="nef"
                     /label=Protein Nef
                     /note="Protein Nef from Human immunodeficiency virus type 1
                     group M subtype D (isolate Z84). Accession#: P12481"
     LTR             8606..9239
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10235..10252)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10406..10994)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11165..12025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTKPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12026..12130)
                     /label=AmpR promoter
     primer_bind     12198..12216
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     primer_bind     complement(12254..12276)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12376..12395
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
ORIGIN
        1 ccggagtact acaaagactg ctgacatcga gctttctaca agggactttc cgctggggac
       61 tttccaggga ggtgtggcct gggcgggact ggggagtggc gagccctcag atgctacata
      121 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
      181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
      241 caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
      301 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta
      361 aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
      421 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
      481 gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg aaaaaattcg
      541 gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg caagcaggga
      601 gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct gtagacaaat
      661 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
      721 tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc
      781 cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc
      841 tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga acctccaggg
      901 gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga
      961 agagaaggct ttcagcccag aagtaatacc catgttttca gcattatcag aaggagccac
     1021 cccacaagat ttaaatacca tgctaaacac agtgggggga catcaagcag ccatgcaaat
     1081 gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc cagtgcatgc
     1141 agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac
     1201 tagtaccctt caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga
     1261 aatctataaa agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac
     1321 cagcattctg gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt
     1381 ctataaaact ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac
     1441 cttgttggtc caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg
     1501 agcgacacta gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc
     1561 aagagttttg gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa
     1621 aggcaatttt aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca
     1681 catagccaaa aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg
     1741 acaccaaatg aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc
     1801 ccacaaggga aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga
     1861 agagagcttc aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga
     1921 caaggaactg tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca
     1981 ataaagatag gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta
     2041 ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt
     2101 tttatcaaag taagacagta tgatcagata ctcatagaaa tctgcggaca taaagctata
     2161 ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag
     2221 attggctgca ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag
     2281 ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca
     2341 ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa
     2401 aatccataca atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa
     2461 ttagtagatt tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga
     2521 ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat
     2581 gcatattttt cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct
     2641 agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg
     2701 aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa
     2761 caaaatccag acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta
     2821 gaaatagggc agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga
     2881 tttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa
     2941 ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact
     3001 gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg
     3061 attaaagtaa ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta
     3121 ccactaacag aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg
     3181 gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg
     3241 caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaag
     3301 tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa
     3361 aaaatagcca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata
     3421 caaaaggaaa catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag
     3481 tgggagtttg tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc
     3541 ataataggag cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga
     3601 aaagcaggat atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca
     3661 aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta
     3721 aacatagtga cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt
     3781 gaatcagagt tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg
     3841 gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagataa attggtcagt
     3901 gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag
     3961 aaatatcaca gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca
     4021 aaagaaatag tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa
     4081 gtagactgta gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc
     4141 ttggtagcag ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca
     4201 gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta
     4261 catacagaca atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg
     4321 gggatcaagc aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct
     4381 atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag
     4441 acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4501 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4561 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4621 gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat
     4681 aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa
     4741 cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattaaca catggaaaag
     4801 attagtaaaa caccatatgt atatttcaag gaaagctaag gactggtttt atagacatca
     4861 ctatgaaagt actaatccaa aaataagttc agaagtacac atcccactag gggatgctaa
     4921 attagtaata acaacatatt ggggtctgca tacaggagaa agagactggc atttgggtca
     4981 gggagtctcc atagaatgga ggaaaaagag atatagcaca caagtagacc ctgacctagc
     5041 agaccaacta attcatctgc actattttga ttgtttttca gaatctgcta taagaaatac
     5101 catattagga cgtatagtta gtcctaggtg tgaatatcaa gcaggacata acaaggtagg
     5161 atctctacag tacttggcac tagcagcatt aataaaacca aaacagataa agccaccttt
     5221 gcctagtgtt aggaaactga cagaggacag atggaacaag ccccagaaga ccaagggcca
     5281 cagagggagc catacaatga atggacacta gagcttttag aggaacttaa gagtgaagct
     5341 gttagacatt ttcctaggat atggctccat aacttaggac aacatatcta tgaaacttac
     5401 ggggatactt gggcaggagt ggaagccata ataagaattc tgcaacaact gctgtttatc
     5461 catttcagaa ttgggtgtcg acatagcaga ataggcgtta ctcgacagag gagagcaaga
     5521 aatggagcca gtagatccta gactagagcc ctggaagcat ccaggaagtc agcctaaaac
     5581 tgcttgtacc aattgctatt gtaaaaagtg ttgctttcat tgccaagttt gtttcatgac
     5641 aaaagcctta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa gagctcatca
     5701 gaacagtcag actcatcaag cttctctatc aaagcagtaa gtagtacatg taatgcaacc
     5761 tataatagta gcaatagtag cattagtagt agcaataata atagcaatag ttgtgtggtc
     5821 catagtaatc atagaatata ggaaaatatt aagacaaaga aaaatagaca ggttaattga
     5881 tagactaata gaaagagcag aagacagtgg caatgagagt gaaggagaag tatcagcact
     5941 tgtggagatg ggggtggaaa tggggcacca tgctccttgg gatattgatg atctgtagtg
     6001 ctacagaaaa attgtgggtc acagtctatt atggggtacc tgtcgccacc atggtgagca
     6061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     6301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     6361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     6421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     6481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggac gagctgtaag
     6781 ctagcaaatt aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag
     6841 gaggggaccc agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta
     6901 attcaacaca actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa
     6961 ataacactga aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca
     7021 tgtggcagga agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt
     7081 catcaaatat tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg
     7141 agatcttcag acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat
     7201 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7261 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7321 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7381 ctgatatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7441 tgcaactcac agtctggggc atcaaacagc tccaggcaag aatcctggct gtggaaagat
     7501 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7561 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca
     7621 tgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7681 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7741 gggcaagttt gaggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7801 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7861 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg
     7921 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     7981 ttcgattagt gaacggatcc ttagcactta tctgggacga tctgcggagc ctgtgcctct
     8041 tcagctacca ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg
     8101 gacgcagggg gtgggaagcc ctcaaatatt ggtggaatct cctacagtat tggagtcagg
     8161 aactaaagaa tagtgctgtt aacttgctca atgccacagc catagcagta gctgagggga
     8221 cagatagggt tatagaagta ttacaagcag cttatagagc tattcgccac atacctagaa
     8281 gaataagaca gggcttggaa aggattttgc tataagatgg gtggcaagtg gtcaaaaagt
     8341 agtgtgattg gatggcctgc tgtaagggaa agaatgagac gagctgagcc agcagcagat
     8401 ggggtgggag cagtatctcg agacctagaa aaacatggag caatcacaag tagcaataca
     8461 gcagctaaca atgctgcttg tgcctggcta gaagcacaag aggaggaaga ggtgggtttt
     8521 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8581 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat
     8641 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     8701 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8761 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8821 agcctgcatg gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc
     8881 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat
     8941 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     9001 actggggagt ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg
     9061 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9121 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9181 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac
     9241 ccaggaggta gaggttgcag tgagccaaga tcgcgccact gcattcgggg atcagagttt
     9301 ttaaggataa cttagtgtgt agggggccag tgagttggag atgaaagcgt agggagtcga
     9361 aggtgtcctt ttgcgccgag tcagttcctg ggtgggggcc acaagatcgg atgagccagt
     9421 ttatcaatcc gggggtgcca gctgatccat gcatggagtg cagggtctgc aaaatatctc
     9481 aagcactgat tgatcttagg ttttacaata gtgatgttac cccaggaaca atttggggaa
     9541 ggtcagaatc ttgtagcctg tagctgcatg actcctaaac cataatttct tttttgtttt
     9601 ttttttttta tttttgagac agggtctcac tctgtcacct aggctggagt gcagtggtgc
     9661 aatcacagct cactgcagcc tcaacgtcgt aagctcaagc gatcctccca cctcagcctg
     9721 cctggtagct gagactacaa gcgacgcccc agttaatttt tgtatttttg gtagaggcag
     9781 cgttttgccg tgtggccctg gctggtctcg aactcctggg ctcaagtgat ccagcctcag
     9841 cctcccaaag tgctgggaca accggggcca gtcactgcac ctggccctaa accataattt
     9901 ctaatctttt ggctaatttg ttagtcctac aaaggcagtc tagtccccag gcaaaaaggg
     9961 ggtttgtttc gggaaagggc tgttactgtc tttgtttcaa actataaact aagttcctcc
    10021 taaacttagt tcggcctaca cccaggaatg aacaaggaga gcttggaggt tagaagcacg
    10081 atggaattgg ttaggtcaga tctctttcac tgtctgagtt ataattttgc aatggtggtt
    10141 caaagactgc ccgcttctga caccagtcgc tgcattaatg aatcggccaa cgcgcgggga
    10201 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10261 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10321 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10441 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    10861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    10921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    10981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    11161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11461 gcaacgttgt tgccattgct acaggcttcg tggtgtcacg ctcgtcgttt ggtatggctt
    11521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    11821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    11881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    11941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    12001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    12061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    12181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    12241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    12301 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    12361 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    12421 aaataccgca cagatgcgta aggagaaaat accgcatcag gccagagtcc ttggtgtgga
    12481 gggagggacc agcgcagctt ccagccatcc acctgatgaa cagaacctag ggaaagcccc
    12541 agttctactt acaccaggaa aggctggaag ggctaatttg gtcccaaaaa agacaagaga
    12601 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
    12661 cagggccagg gatcagatat ccactgacct ttggatggtg cttcaagtta gtaccagttg
    12721 aaccagagca agtagaagag gccaatgaag gagagaacaa cagcttgtta caccctatga
    12781 gccagcatgg gatggaggac ccggagggag aagtattagt gtggaagttt gacagcctcc
    12841 tagcatttcg tcacatggcc cgagagctgc at
//
