LOCUS       40924_14765       10972 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pdLucLRH-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10972)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     In vivo imaging of nuclear receptor signaling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10972)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2004) Institut de Genetique et de Biologie 
            Moleculaire et Cellulaire, 1 rue Laurent Fries, Illkirch 67404, 
            France
REFERENCE   3  (bases 1 to 10972)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10972)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2004) Institut de Genetique et de Biologie Moleculaire et 
            Cellulaire, 1 rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10972
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      181..636
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /label=AmpR promoter
     CDS             819..1676
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1850..2438
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2902..4124
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     misc_feature    4125..5334
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     protein_bind    5385..5479
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5510..5606
                     /label=LRH-1 binding site
                     /bound_moiety="LRH-1"
     regulatory      5625..6734
                     /label=Wnt-1 promoter
                     /note="Wnt-1 promoter"
                     /regulatory_class="promoter"
     CDS             6776..8425
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(8622..8743)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8855..10945
                     /label=3' flanking sequence of Wnt-1
                     /note="3' flanking sequence of Wnt-1"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gcgacgcgtc gactctagag ccccatcctc actgactccg
     2941 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     3001 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     3061 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     3121 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     3181 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     3241 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     3301 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     3361 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     3421 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     3481 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     3541 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     3601 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     3661 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     3721 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     3781 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     3841 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     3901 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3961 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4021 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4081 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc
     4141 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     4201 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     4261 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     4321 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     4381 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     4441 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     4501 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     4561 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     4621 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     4681 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     4741 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     4801 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     4861 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     4921 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     4981 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     5041 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     5101 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5161 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     5221 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     5281 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     5341 ttaactacgt acggccgcgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc
     5401 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     5461 cggagtactg tcctccgagc ggagactcta gcgagcgccg gaggcggcca agcaaccttg
     5521 atggcatggg cccagagcaa ccttgactgc cccagcggcc agagcaacct tgatggcatg
     5581 ggcccagagc aaccttgact gccccagcgg ccgctcgact catcacgtct cctgtcacag
     5641 ccccacctcc ttcctgatac actgcgttaa gtggtgactg taacaaaatg acttcatgct
     5701 ctccctgtcc tgagccaaat tacacaatta tttggaaagg gctcaaaatg ttcttcgtta
     5761 gaagtttctg gatacaccaa tacacaggag cgtgcaccct cagaacacat gtacactttg
     5821 acttaatctc acgggtgaca caccgacgct tacactcccc ctagcccaca gaggcaaact
     5881 gctgggcgct tctgagtttc tcactgccac cagctcggtt tgctcagcct acccccgcac
     5941 cccgcgcccg ggaatccctg accacagctc cacccatgct ctgtctcctt cttttccttc
     6001 tctgtccagc cgtcggggtt cctgggtgag gaagtgtctc cacggagtcg ctggctagaa
     6061 ccacaacttt catcctgcca ttcagaatag ggaagagaag agaccacagc gtagggggga
     6121 cagaggagac ggacttcgag aggacagccc caccggcgcg tgtgggggag gcaatccagg
     6181 ctgcaaacag gttgtcccca gcgcattgtc cccgcgcccc ctggcggatg ctggtccccg
     6241 acgggctccg gacgcgcaga agagtgaggc cggcgcgcgt gggaggccat cccaagggga
     6301 ggggtcggcg gccagtgcag acctggaggc ggggccacca ggcagggggc gggggtgagc
     6361 cccgacggtt agcctgtcag ctctttgctc agaccggcaa gagccacagc ttcgctcgcc
     6421 actcattgtc tgtggccctg accagtgcgc cctggtgctt ttagtgccgc ccgggcccgg
     6481 aggggcagcc tcttctcact gcagtcagcg ccgcaactat aagaggccta taagaggcgg
     6541 tgcctcccgc agtggctgct tcagcccagc agccaggaca gcgaaccatg ctgcctgcgg
     6601 cccgcctcca gacttattag agccagcctg ggaactcgca tcactgccct caccgctgtg
     6661 tccagtccca ccgtcgcgga cagcaaccac agtcgtcaga accgcagcac agaaccagca
     6721 aggccaggca ggcctcgcga tagcttggca ttccggtact gttggtaaag ccaccatgga
     6781 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
     6841 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     6901 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
     6961 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     7021 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     7081 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     7141 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     7201 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     7261 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     7321 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     7381 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     7441 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     7501 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     7561 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     7621 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     7681 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     7741 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     7801 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     7861 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     7921 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     7981 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     8041 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     8101 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     8161 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     8221 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     8281 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     8341 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     8401 caagaagggc ggaaagatcg ccgtgtcaag ccatggcttc ccgccggcgg tggcggcgca
     8461 ggatgatggc acgctgccca tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc
     8521 agcctgtgct tctgctagga tcaatgtgta ggcggccatc ggatcgcatt gttggccgcg
     8581 actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     8641 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     8701 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     8761 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     8821 ctacaaatgt ggtaaaatcg ataaggatcc gtcgattttg gtcgcctctt tggccgagag
     8881 ttcgtggact ccggggagaa ggggcgggac ctacgcttcc tcatgaacct tcacaacaac
     8941 gaggcagggc gaacggtacg tcggtgtgtc cggaaccaat ggcaggggag atgtaagaca
     9001 ggtgcacggg gacagaggca cagggagggg cttcccgaga gagtgggact ctaggaggga
     9061 agacagagaa gaggtggtgg ttgagggcaa agaggttcct gagctgatga cagaacagaa
     9121 gagattagca ggctatcaac acgtgggatg tattgagatg gctccatggc acacttttga
     9181 aagataaaag tgacttgctg gcgtggagca gagtctggcc gaatgtccct atctcagcgg
     9241 gccattttgc acttcctctc tcccgagctt agtcacacct ggaccttggc tgaagtttcc
     9301 acagcatcga cgtgacccgg gtggggtggg ggtggggaag tatgggtggt ggttcgtggg
     9361 atgttggctt tgaccttttc ttccctcctc ccctcgtccc ctcctccccc agaccgtgtt
     9421 ctctgagatg cgccaagagt gcaaatgcca cgggatgtcc ggctcctgca cggtgcgcac
     9481 gtgttggatg cggctgccca cgctgcgcgc tgtgggcgac gtgctgcgcg accgcttcga
     9541 cggcgcctcc cgcgtccttt acggcaaccg aggcagcaac cgcgcctcgc gggcggagct
     9601 gctgcgcctg gagcccgaag accccgcgca caagcctccc tcccctcacg acctcgtcta
     9661 cttcgagaaa tcgcccaact tctgcacgta cagtggccgc ctgggcacag ctggcacagc
     9721 tggacgagct tgcaacagct cgtctcccgc gctggacggc tgtgagctgc tgtgctgtgg
     9781 ccgaggccac cgcacgcgca cgcagcgcgt cacggagcgc tgcaactgca ccttccactg
     9841 gtgctgccac gtcagctgcc gcaactgcac gcacacgcgc gttctgcacg agtgtctatg
     9901 aggtgccgcg cctccgggaa cgggaacgct ctcttccagt tctcagacac actcgctggt
     9961 cctgatgttt gcccacccta ccgcgtccag ccacagtccc agggttcata gcgatccatc
    10021 tctcccacct cctacctggg gactcctgaa accacttgcc tgagtcggct cgaacccttt
    10081 tgccatcctg agggccctga cccagcctac ctccctccct ctttgaggga gactcctttt
    10141 gcactgcccc ccaatttggc cagagggtga gagaaagatt cttcttctgg ggtgggggtg
    10201 gggaggtcaa ctcttgaagg tgttgcggtt cctgatgtat tttgcgctgt gacctctttg
    10261 ggtattatca cctttccttg tctctcgggt ccctataggt cccttgagtt ctctaaccag
    10321 cacctctggg cttcaaggcc tttcccctcc cacctgtagc tgaagagttt ccgagttgaa
    10381 agggcacgga aagctaagtg ggaaaggagg ttgctggacc cagcagcaaa accctacatt
    10441 ctccttgtct ctgcctcgga gccattgaac agctgtgaac catgcctccc tcagcctcct
    10501 cccacccctt cctgtcctgc ctcctcatca ctgtgtaaat aatttgcacc gaaatgtggc
    10561 cgcagagcca cgcgttcggt tatgtaaata aaactattta ttgtgctggg ttccagcctg
    10621 ggttgcagag accaccctca ccccacctca ctgctcctct gttctgctcg ccagtccttt
    10681 tgttatccga ccttttttct cttttaccca gcttctcata ggcgcccttg cccaccggat
    10741 cagtatttcc ttccactgta gctattagtg gctcctcgcc cccaccaatg tagtatcttc
    10801 ctctgaggaa taaaatatct atttttatca acgactctgg tccttgaatc cagaacacag
    10861 catggcttcc aacgtcctct tcccttccaa tggacttgct tctcttctca tagccaaaca
    10921 aaagagatag agttgttgaa gatctgtcga cgacgtcggg cccaattcgc cc
//
