LOCUS       40924_14755       10961 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pdLucFXR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Houten SM, Volle DH, Cummins CL, Mangelsdorf DJ, Auwerx J.
  TITLE     In vivo imaging of farnesoid X receptor activity reveals the ileum 
            as the primary bile acid signaling tissue
  JOURNAL   Mol. Endocrinol. 21 (6), 1312-1323 (2007)
  PUBMED    17426284
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   Houten SM, Auwerx J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2004) Institut de Genetique et de Biologie 
            Moleculaire et Cellulaire, 1 rue Laurent Fries, Illkirch 67404, 
            France
REFERENCE   3  (bases 1 to 10961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Endocrinol."; date: "2007"; volume: "21"; issue: "6"; pages: 
            "1312-1323"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2004) Institut de Genetique et de Biologie Moleculaire et 
            Cellulaire, 1 rue Laurent Fries, Illkirch 67404, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      181..636
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /label=AmpR promoter
     CDS             819..1676
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1850..2438
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    2902..4124
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     misc_feature    4125..5334
                     /label=beta-globin insulator
                     /note="beta-globin insulator"
     protein_bind    5385..5479
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5510..5595
                     /label=FXR binding site
                     /bound_moiety="FXR"
     regulatory      5614..6723
                     /label=Wnt-1 promoter
                     /note="Wnt-1 promoter"
                     /regulatory_class="promoter"
     CDS             6765..8414
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(8611..8732)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8844..10934
                     /label=3' flanking sequence of Wnt-1
                     /note="3' flanking sequence of Wnt-1"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gcgacgcgtc gactctagag ccccatcctc actgactccg
     2941 tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat
     3001 ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt
     3061 gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac tgagggacat
     3121 ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca
     3181 gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc
     3241 agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc
     3301 cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg
     3361 tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat cttcttatcc
     3421 ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg
     3481 tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc cgtgatgcac
     3541 tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata
     3601 ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat atgaggaggc
     3661 agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc
     3721 cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg
     3781 gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc tcctttgcaa
     3841 cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     3901 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     3961 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     4021 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     4081 ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag ccccatcctc
     4141 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     4201 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     4261 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     4321 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     4381 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     4441 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     4501 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     4561 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     4621 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     4681 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     4741 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     4801 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     4861 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     4921 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     4981 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     5041 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     5101 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     5161 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     5221 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     5281 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     5341 ttaactacgt acggccgcgg atccaagctt gcatgcctgc aggtcggagt actgtcctcc
     5401 gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag
     5461 cggagtactg tcctccgagc ggagactcta gcgagcgccg gaggcggcca agagaggtca
     5521 ctgaccttgg catgggccca gagaggtcac tgacctctgc cccagcggcc agagaggtca
     5581 ctgacctctg ccccagcggc cgctcgactc atcacgtctc ctgtcacagc cccacctcct
     5641 tcctgataca ctgcgttaag tggtgactgt aacaaaatga cttcatgctc tccctgtcct
     5701 gagccaaatt acacaattat ttggaaaggg ctcaaaatgt tcttcgttag aagtttctgg
     5761 atacaccaat acacaggagc gtgcaccctc agaacacatg tacactttga cttaatctca
     5821 cgggtgacac accgacgctt acactccccc tagcccacag aggcaaactg ctgggcgctt
     5881 ctgagtttct cactgccacc agctcggttt gctcagccta cccccgcacc ccgcgcccgg
     5941 gaatccctga ccacagctcc acccatgctc tgtctccttc ttttccttct ctgtccagcc
     6001 gtcggggttc ctgggtgagg aagtgtctcc acggagtcgc tggctagaac cacaactttc
     6061 atcctgccat tcagaatagg gaagagaaga gaccacagcg taggggggac agaggagacg
     6121 gacttcgaga ggacagcccc accggcgcgt gtgggggagg caatccaggc tgcaaacagg
     6181 ttgtccccag cgcattgtcc ccgcgccccc tggcggatgc tggtccccga cgggctccgg
     6241 acgcgcagaa gagtgaggcc ggcgcgcgtg ggaggccatc ccaaggggag gggtcggcgg
     6301 ccagtgcaga cctggaggcg gggccaccag gcagggggcg ggggtgagcc ccgacggtta
     6361 gcctgtcagc tctttgctca gaccggcaag agccacagct tcgctcgcca ctcattgtct
     6421 gtggccctga ccagtgcgcc ctggtgcttt tagtgccgcc cgggcccgga ggggcagcct
     6481 cttctcactg cagtcagcgc cgcaactata agaggcctat aagaggcggt gcctcccgca
     6541 gtggctgctt cagcccagca gccaggacag cgaaccatgc tgcctgcggc ccgcctccag
     6601 acttattaga gccagcctgg gaactcgcat cactgccctc accgctgtgt ccagtcccac
     6661 cgtcgcggac agcaaccaca gtcgtcagaa ccgcagcaca gaaccagcaa ggccaggcag
     6721 gcctcgcgat agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
     6781 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
     6841 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     6901 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
     6961 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     7021 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     7081 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
     7141 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
     7201 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     7261 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
     7321 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
     7381 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
     7441 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     7501 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     7561 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
     7621 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
     7681 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
     7741 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
     7801 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     7861 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     7921 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
     7981 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     8041 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
     8101 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
     8161 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     8221 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     8281 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     8341 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     8401 gaaagatcgc cgtgtcaagc catggcttcc cgccggcggt ggcggcgcag gatgatggca
     8461 cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca gcctgtgctt
     8521 ctgctaggat caatgtgtag gcggccatcg gatcgcattg ttggccgcga ctctagagtc
     8581 ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     8641 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     8701 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8761 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     8821 gtaaaatcga taaggatccg tcgattttgg tcgcctcttt ggccgagagt tcgtggactc
     8881 cggggagaag gggcgggacc tacgcttcct catgaacctt cacaacaacg aggcagggcg
     8941 aacggtacgt cggtgtgtcc ggaaccaatg gcaggggaga tgtaagacag gtgcacgggg
     9001 acagaggcac agggaggggc ttcccgagag agtgggactc taggagggaa gacagagaag
     9061 aggtggtggt tgagggcaaa gaggttcctg agctgatgac agaacagaag agattagcag
     9121 gctatcaaca cgtgggatgt attgagatgg ctccatggca cacttttgaa agataaaagt
     9181 gacttgctgg cgtggagcag agtctggccg aatgtcccta tctcagcggg ccattttgca
     9241 cttcctctct cccgagctta gtcacacctg gaccttggct gaagtttcca cagcatcgac
     9301 gtgacccggg tggggtgggg gtggggaagt atgggtggtg gttcgtggga tgttggcttt
     9361 gaccttttct tccctcctcc cctcgtcccc tcctccccca gaccgtgttc tctgagatgc
     9421 gccaagagtg caaatgccac gggatgtccg gctcctgcac ggtgcgcacg tgttggatgc
     9481 ggctgcccac gctgcgcgct gtgggcgacg tgctgcgcga ccgcttcgac ggcgcctccc
     9541 gcgtccttta cggcaaccga ggcagcaacc gcgcctcgcg ggcggagctg ctgcgcctgg
     9601 agcccgaaga ccccgcgcac aagcctccct cccctcacga cctcgtctac ttcgagaaat
     9661 cgcccaactt ctgcacgtac agtggccgcc tgggcacagc tggcacagct ggacgagctt
     9721 gcaacagctc gtctcccgcg ctggacggct gtgagctgct gtgctgtggc cgaggccacc
     9781 gcacgcgcac gcagcgcgtc acggagcgct gcaactgcac cttccactgg tgctgccacg
     9841 tcagctgccg caactgcacg cacacgcgcg ttctgcacga gtgtctatga ggtgccgcgc
     9901 ctccgggaac gggaacgctc tcttccagtt ctcagacaca ctcgctggtc ctgatgtttg
     9961 cccaccctac cgcgtccagc cacagtccca gggttcatag cgatccatct ctcccacctc
    10021 ctacctgggg actcctgaaa ccacttgcct gagtcggctc gaaccctttt gccatcctga
    10081 gggccctgac ccagcctacc tccctccctc tttgagggag actccttttg cactgccccc
    10141 caatttggcc agagggtgag agaaagattc ttcttctggg gtgggggtgg ggaggtcaac
    10201 tcttgaaggt gttgcggttc ctgatgtatt ttgcgctgtg acctctttgg gtattatcac
    10261 ctttccttgt ctctcgggtc cctataggtc ccttgagttc tctaaccagc acctctgggc
    10321 ttcaaggcct ttcccctccc acctgtagct gaagagtttc cgagttgaaa gggcacggaa
    10381 agctaagtgg gaaaggaggt tgctggaccc agcagcaaaa ccctacattc tccttgtctc
    10441 tgcctcggag ccattgaaca gctgtgaacc atgcctccct cagcctcctc ccaccccttc
    10501 ctgtcctgcc tcctcatcac tgtgtaaata atttgcaccg aaatgtggcc gcagagccac
    10561 gcgttcggtt atgtaaataa aactatttat tgtgctgggt tccagcctgg gttgcagaga
    10621 ccaccctcac cccacctcac tgctcctctg ttctgctcgc cagtcctttt gttatccgac
    10681 cttttttctc ttttacccag cttctcatag gcgcccttgc ccaccggatc agtatttcct
    10741 tccactgtag ctattagtgg ctcctcgccc ccaccaatgt agtatcttcc tctgaggaat
    10801 aaaatatcta tttttatcaa cgactctggt ccttgaatcc agaacacagc atggcttcca
    10861 acgtcctctt cccttccaat ggacttgctt ctcttctcat agccaaacaa aagagataga
    10921 gttgttgaag atctgtcgac gacgtcgggc ccaattcgcc c
//
