LOCUS       V000591                 9333 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000591
VERSION     V000591
KEYWORDS    pcDNA5-Flag-BRD4-WT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Shu S, Lin CY, He HH, Witwicki RM, Tabassum DP, Roberts JM,
            Janiszewska M, Huh SJ, Liang Y, Ryan J, Doherty E, Mohammed H, Guo
            H, Stover DG, Ekram MB, Peluffo G, Brown J, D'Santos C, Krop IE,
            Dillon D, McKeown M, Ott C, Qi J, Ni M, Rao PK, Duarte M, Wu SY,
            Chiang CM, Anders L, Young RA, Winer EP, Letai A, Barry WT, Carroll
            JS, Long HW, Brown M, Liu XS, Meyer CA, Bradner JE, Polyak K
  TITLE     Response and resistance to BET bromodomain inhibitors in
            triple-negative breast cancer.
  JOURNAL   Nature. 2016 Jan 21;529(7586):413-7. doi: 10.1038/nature16508. Epub
            2016 Jan 6.
   PUBMED   26735014
REFERENCE   2  (bases 1 to 9333)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1038/nature16508"; journalName: "Nature"; date: "2016-01-21-
            21"; volume: "529"; issue: "7586"; pages: "413-7"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..42)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        214..593
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        594..797
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    799..817
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    820..838
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     848..872
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             971..994
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1001..5086
                     /gene="BRD4"
                     /label="Bromodomain-containing protein 4"
                     /note="Bromodomain-containing protein 4 from Homo sapiens.
                     Accession#: O60885"
     polyA_signal    5270..5494
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5627..5646)
                     /label="F1ori-R"
                     /note="F1 origin, reverse primer"
     protein_bind    5778..5825
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5833..6852
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    6985..7118
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7155..7171)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7179..7195)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7203..7233)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7248..7269)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7386..7403)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7557..8145)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8319..9176)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9177..9281)
                     /label="AmpR promoter"
ORIGIN
        1 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
       61 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
      121 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      181 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      241 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      301 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      361 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      421 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      481 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      541 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      601 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      661 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      721 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      781 tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc
      841 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      901 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      961 ttccaccatg gattacaagg atgacgacga taagggatct atgtctgcgg agagcggccc
     1021 tgggacgaga ttgagaaatc tgccagtaat gggggatgga ctagaaactt cccaaatgtc
     1081 tacaacacag gcccaggccc aaccccagcc agccaacgca gccagcacca accccccgcc
     1141 cccagagacc tccaacccta acaagcccaa gaggcagacc aaccaactgc aatacctgct
     1201 cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc agcagcctgt
     1261 ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc ctatggatat
     1321 gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg aatgtatcca
     1381 ggacttcaac actatgttta caaattgtta catctacaac aagcctggag atgacatagt
     1441 cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc tacccacaga
     1501 agaaaccgag atcatgatag tccaggcaaa aggaagagga cgtgggagga aagaaacagg
     1561 gacagcaaaa cctggcgttt ccacggtacc aaacacaact caagcatcga ctcctccgca
     1621 gacccagacc cctcagccga atcctcctcc tgtgcaggcc acgcctcacc ccttccctgc
     1681 cgtcaccccg gacctcatcg tccagacccc tgtcatgaca gtggtgcctc cccagccact
     1741 gcagacgccc ccgccagtgc ccccccagcc acaaccccca cccgctccag ctccccagcc
     1801 cgtacagagc cacccaccca tcatcgcggc caccccacag cctgtgaaga caaagaaggg
     1861 agtgaagagg aaagcagaca ccaccacccc caccaccatt gaccccattc acgagccacc
     1921 ctcgctgccc ccggagccca agaccaccaa gctgggccag cggcgggaga gcagccggcc
     1981 tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag cacccagcac cagagaagag
     2041 cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc ctcaaggaga tgtttgccaa
     2101 gaagcacgcc gcctacgcct ggcccttcta caagcctgtg gacgtggagg cactgggcct
     2161 acacgactac tgtgacatca tcaagcaccc catggacatg agcacaatca agtctaaact
     2221 ggaggcccgt gagtaccgtg atgctcagga gtttggtgct gacgtccgat tgatgttctc
     2281 caactgctat aagtacaacc ctcctgacca tgaggtggtg gccatggccc gcaagctcca
     2341 ggatgtgttc gaaatgcgct ttgccaagat gccggacgag cctgaggagc cagtggtggc
     2401 cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct catccagcga
     2461 cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg aggaggagcg
     2521 agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc agcttgcagc
     2581 cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga aggaaaagaa
     2641 aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca aagccaagga
     2701 acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga gcaagaagga
     2761 gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag aggacaagtg
     2821 caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca agctccccgg
     2881 cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc tgaagaattc
     2941 caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac tgcgtgagct
     3001 ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg agaaagttga
     3061 tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg agagctccag
     3121 tgagtccagc tcctctgaca gcgaagactc cgaaacagag atggctccga agtcaaaaaa
     3181 gaaggggcac cccgggaggg agcagaagaa gcaccatcat caccaccatc agcagatgca
     3241 gcaggccccg gctcctgtgc cccagcagcc gcccccgcct ccccagcagc ccccaccgcc
     3301 tccacctccg cagcagcaac agcagccgcc acccccgcct cccccaccct ccatgccgca
     3361 gcaggcagcc ccggcgatga agtcctcgcc cccacccttc attgccaccc aggtgcccgt
     3421 cctggagccc cagctcccag gcagcgtctt tgaccccatc ggccacttca cccagcccat
     3481 cctgcacctg ccgcagcctg agctgccccc tcacctgccc cagccgcctg agcacagcac
     3541 tccaccccat ctcaaccagc acgcagtggt ctctcctcca gctttgcaca acgcactacc
     3601 ccagcagcca tcacggccca gcaaccgagc cgctgccctg cctcccaagc ccgcccggcc
     3661 cccagccgtg tcaccagcct tgacccaaac acccctgctc ccacagcccc ccatggccca
     3721 acccccccaa gtgctgctgg aggatgaaga gccacctgcc ccacccctca cctccatgca
     3781 gatgcagctg tacctgcagc agctgcagaa ggtgcagccc cctacgccgc tactcccttc
     3841 cgtgaaggtg cagtcccagc ccccaccccc cctgccgccc ccaccccacc cctctgtgca
     3901 gcagcagctg cagcagcagc cgccaccacc cccaccaccc cagccccagc ctccacccca
     3961 gcagcagcat cagccccctc cacggcccgt gcacttgcag cccatgcagt tttccaccca
     4021 catccaacag cccccgccac cccagggcca gcagcccccc catccgcccc caggccagca
     4081 gccacccccg ccgcagcctg ccaagcctca gcaagtcatc cagcaccacc attcaccccg
     4141 gcaccacaag tcggacccct actcaaccgg tcacctccgc gaagccccct ccccgcttat
     4201 gatacattcc ccccagatgt cacagttcca gagcctgacc caccagtctc caccccagca
     4261 aaacgtccag cctaagaaac aggagctgcg tgctgcctcc gtggtccagc cccagcccct
     4321 cgtggtggtg aaggaggaga agatccactc acccatcatc cgcagcgagc ccttcagccc
     4381 ctcgctgcgg ccggagcccc ccaagcaccc ggagagcatc aaggcccccg tccacctgcc
     4441 ccagcggccg gaaatgaagc ctgtggatgt cgggaggcct gtgatccggc ccccagagca
     4501 gaacgcaccg ccaccagggg cccctgacaa ggacaaacag aaacaggagc cgaagactcc
     4561 agttgcgccc aaaaaggacc tgaaaatcaa gaacatgggc tcctgggcca gcctagtgca
     4621 gaagcatccg accaccccct cctccacagc caagtcatcc agcgacagct tcgagcagtt
     4681 ccgccgcgcc gctcgggaga aagaggagcg tgagaaggcc ctgaaggctc aggccgagca
     4741 cgctgagaag gagaaggagc ggctgcggca ggagcgcatg aggagccgag aggacgagga
     4801 tgcgctggag caggcccggc gggcccatga ggaggcacgt cggcgccagg agcagcagca
     4861 gcagcagcgc caggagcaac agcagcagca gcaacagcaa gcagctgcgg tggctgccgc
     4921 cgccacccca caggcccaga gctcccagcc ccagtccatg ctggaccagc agagggagtt
     4981 ggcccggaag cgggagcagg agcgaagacg ccgggaagcc atggcagcta ccattgacat
     5041 gaatttccag agtgatctat tgtcaatatt tgaagaaaat cttttctgag cgcacctagg
     5101 tggcttctga ctttgatttt ctggcaaaac attgactttc catagtgtta ggggcggtgg
     5161 tggaggtggg atcagcggcc aggggatgcc tcagggcctg gccctcctgc atgcaagctt
     5221 cagctgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5281 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5341 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5401 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5461 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5521 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5581 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5641 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5701 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5761 gatggttcac gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga
     5821 acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     5881 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     5941 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     6001 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     6061 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     6121 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     6181 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     6241 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     6301 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     6361 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     6421 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6481 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6541 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6601 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6661 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     6721 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6781 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6841 agggcaaagg aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6961 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7081 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8281 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8341 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8401 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8461 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8521 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8581 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8641 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8701 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8761 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8821 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8881 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8941 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9001 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9061 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9121 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9181 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9241 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9301 ccacctgacg tcgacggatc gggagatctc ccg
//
