LOCUS       V008584                 6407 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008584
VERSION     V008584
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             956..1882
                     /gene="Cd86"
                     /label="T-lymphocyte activation antigen CD86"
                     /note="T-lymphocyte activation antigen CD86 from Mus
                     musculus. Accession#: P42082"
     promoter        complement(1959..1977)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2003..2227
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2273..2701
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2715..3045
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3112..3903
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4080..4213
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4250..4266)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4274..4290)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4298..4328)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4343..4364)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4652..5240)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5414..6271)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6272..6376)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcggcttac ccacgatgga
      961 ccccagatgc accatgggct tggcaatcct tatctttgtg acagtcttgc tgatctcaga
     1021 tgctgtttcc gtggagacgc aagcttattt caatgggact gcatatctgc cgtgcccatt
     1081 tacaaaggct caaaacataa gcctgagtga gctggtagta ttttggcagg accagcaaaa
     1141 gttggttctg tacgagcact atttgggcac agagaaactt gatagtgtga atgccaagta
     1201 cctgggccgc acgagctttg acaggaacaa ctggactcta cgacttcaca atgttcagat
     1261 caaggacatg ggctcgtatg attgttttat acaaaaaaag ccacccacag gatcaattat
     1321 cctccaacag acattaacag aactgtcagt gatcgccaac ttcagtgaac ctgaaataaa
     1381 actggctcag aatgtaacag gaaattctgg cataaatttg acctgcacgt ctaagcaagg
     1441 tcacccgaaa cctaagaaga tgtattttct gataactaat tcaactaatg agtatggtga
     1501 taacatgcag atatcacaag ataatgtcac agaactgttc agtatctcca acagcctctc
     1561 tctttcattc ccggatggtg tgtggcatat gaccgttgtg tgtgttctgg aaacggagtc
     1621 aatgaagatt tcctccaaac ctctcaattt cactcaagag tttccatctc ctcaaacgta
     1681 ttggaaggag attacagctt cagttactgt ggccctcctc cttgtgatgc tgctcatcat
     1741 tgtatgtcac aagaagccga atcagcctag caggcccagc aacacagcct ctaagttaga
     1801 gcgggatagt aacgctgaca gagagactat caacctgaag gaacttgaac cccaaattgc
     1861 ttcagcaaaa ccaaatgcag agtgaaggca gtgaagccga attctgcaga tatccatcac
     1921 actggcggcc gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct
     1981 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2041 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2101 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2161 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2221 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2401 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     2461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2641 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2701 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
     2761 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2821 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2881 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2941 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     3001 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     3061 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     4081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4201 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4381 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4441 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     4861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6361 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
