LOCUS       40924_10186        7613 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pcDNA3flag-Tax1BP1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             922..945
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             967..3210
                     /codon_start=1
                     /note="unnamed protein product; hTAX1BP1"
                     /protein_id="SJL87427.1"
                     /translation="MTSFQEVPLQTSNFAHVIFQNVAKSYLPNAHLECHYTLTPYIHPH
                     PKDWVGIFKVGWSTARDYYTFLWSPMPEHYVEGSTVNCVLAFQGYYLPNDDGEFYQFCY
                     VTHKGEIRGASTPFQFRASSPVEELLTMEDEGNSDMLVVTTKAGLLELKIEKTMKEKEE
                     LLKLIAVLEKETAQLREQVGRMERELNHEKERCDQLQAEQKGLTEVTQSLKMENEEFKK
                     RFSDATSKAHQLEEDIVSVTHKAIEKETELDSLKDKLKKAQHEREQLECQLKTEKDEKE
                     LYKVHLKNTEIENTKLMSEVQTLKNLDGNKESVITHFKEEIGRLQLCLAEKENLQRTFL
                     LTTSSKEDTCFLKEQLRKAEEQVQATRQEVVFLAKELSDAVNVRDRTMADLHTARLENE
                     KVKKQLADAVAELKLNAMKKDQDKTDTLEHELRREVEDLKLRLQMAADHYKEKFKECQR
                     LQKQINKLSDQSANNNNVFTKKTGNQQKVNDASVNTDPATSASTVDVKPSPSAAEADFD
                     IVTKGQVCEMTKEIADKTEKYNKCKQLLQDEKAKCNKYADELAKMELKWKEQVKIAENV
                     KLELAEVQDNYKELKRSLENPAERKMEDGADGAFYPDEIQRPPVRVPSWGLEDNVVCSQ
                     PARNFSRPDGLEDSEDSKEDENVPTAPDPPSQHLRGHGTGFCFDSSFDVHKKCPLCELM
                     FPPNYDQSKFEEHVESHWKVCPMCSEQFPPDYDQQVFERHVQTHFDQNVLNFD"
     promoter        complement(3238..3256)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3282..3506
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3552..3980
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4033..4243
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             4318..5109
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5286..5419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5456..5472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5480..5496)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5504..5534)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5549..5570)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5858..6446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6620..7477)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7478..7582)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccaccgccat ggactacaag gacgacgacg ataagggcga attctgcaga
      961 tatcatatga catcctttca agaagtccca ttgcagactt ccaactttgc ccatgtcatc
     1021 tttcaaaatg tggccaagag ttaccttcct aatgcacacc tggaatgtca ttacacctta
     1081 actccatata ttcatccaca tccaaaagat tgggttggta tattcaaggt tggatggagt
     1141 actgctcgtg attattacac gtttttatgg tcccctatgc ctgaacatta tgtggaagga
     1201 tcaacagtca attgtgtact agcattccaa ggatattacc ttccaaatga tgatggagaa
     1261 ttttatcagt tctgttacgt tacccataag ggtgaaattc gtggagcaag tacacctttc
     1321 cagtttcgag cttcttctcc agttgaagag ctgcttacta tggaagatga aggaaattct
     1381 gacatgttag tggtgaccac aaaagcaggc cttcttgagt tgaaaattga gaaaaccatg
     1441 aaagaaaaag aagaactgtt aaagttaatt gccgttctgg aaaaagaaac agcacaactt
     1501 cgagaacaag ttgggagaat ggaaagagaa cttaaccatg agaaagaaag atgtgaccaa
     1561 ctgcaagcag aacaaaaggg tcttactgaa gtaacacaaa gcttaaaaat ggaaaatgaa
     1621 gagtttaaga agaggttcag tgatgctaca tccaaagccc atcagcttga ggaagatatt
     1681 gtgtcagtaa cacataaagc aattgaaaaa gaaaccgaat tagacagttt aaaggacaaa
     1741 ctcaagaagg cacaacatga aagagaacaa cttgaatgtc agttgaagac agagaaggat
     1801 gaaaaggaac tttataaggt acatttgaag aatacagaaa tagaaaatac caagcttatg
     1861 tcagaggtcc agactttaaa aaatttagat gggaacaaag aaagcgtgat tactcatttc
     1921 aaagaagaga ttggcaggct gcagttatgt ttggctgaaa aggaaaatct gcaaagaact
     1981 ttcctgctta caacctcaag taaagaagat acttgttttt taaaggagca acttcgtaaa
     2041 gcagaggaac aggttcaggc aactcggcaa gaagttgtct ttctggctaa agaactcagt
     2101 gatgctgtca acgtacgaga cagaacgatg gcagacctgc atactgcacg cttggaaaac
     2161 gagaaagtga aaaagcagtt agctgatgca gtggcagaac ttaaactaaa tgctatgaaa
     2221 aaagatcagg acaagactga tacactggaa cacgaactaa gaagagaagt tgaagatctg
     2281 aaactccgtc ttcagatggc tgcagaccat tataaagaaa aatttaagga atgccaaagg
     2341 ctccaaaaac aaataaacaa actttcagat caatcagcta ataataataa tgtcttcaca
     2401 aagaaaacgg ggaatcagca gaaagtgaat gatgcttcag taaacacaga cccagccact
     2461 tctgcctcta ctgtagatgt aaagccatca ccttctgcag cagaggcaga ttttgacata
     2521 gtaacaaagg ggcaagtctg tgaaatgacc aaagaaattg ctgacaaaac agaaaagtat
     2581 aataaatgta aacaactctt gcaggatgag aaagcaaaat gcaataaata tgctgatgaa
     2641 cttgcaaaaa tggagctgaa atggaaagaa caagtgaaaa ttgctgaaaa tgtaaaactt
     2701 gaactagctg aagtacagga caattataaa gaacttaaaa ggagtctaga aaatccagca
     2761 gaaaggaaaa tggaagatgg agcagatggt gctttttacc cagatgaaat acaaaggcca
     2821 cctgtcagag tcccctcttg gggactggaa gacaatgttg tctgcagcca gcctgctcga
     2881 aactttagtc ggcctgatgg cttagaggac tctgaggata gcaaagaaga tgagaatgtg
     2941 cctactgctc ctgatcctcc aagtcaacat ttacgtgggc atgggacagg cttttgcttt
     3001 gattccagct ttgatgttca caagaagtgt cccctctgtg agttaatgtt tcctcctaac
     3061 tatgatcaga gcaaatttga agaacatgtt gaaagtcact ggaaggtgtg cccgatgtgc
     3121 agcgagcagt tccctcctga ctatgaccag caggtgtttg aaaggcatgt gcagacccat
     3181 tttgatcaga atgttctaaa ttttgactga ctcgagcatg catctagagg gccctattct
     3241 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     3301 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3361 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3421 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3481 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3541 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3601 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3661 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     3721 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3781 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3841 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     3901 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     3961 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4021 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4081 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4141 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4201 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4261 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4321 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     4381 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     4441 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     4501 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     4561 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     4621 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     4681 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     4741 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     4801 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     4861 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     4921 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     4981 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5041 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5101 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5161 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5221 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5281 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5401 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     5461 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5521 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5581 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5641 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5701 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5761 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5821 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5881 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5941 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6001 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     6061 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6121 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6181 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6241 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6301 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6361 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6481 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6541 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6601 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6661 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6721 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6781 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6841 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6901 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6961 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7021 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7081 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7141 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7201 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7261 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7321 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7381 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7441 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7501 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7561 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
