LOCUS       V016097                 6904 bp    DNA     circular SYN 26-MAY-2020
DEFINITION  Exported.
ACCESSION   V016097
VERSION     V016097
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Han X, Yang J, Zeng F, Weng J, Zhang Y, Peng Q, Shen L, Ding S, Liu
            K, Gao Y.
  TITLE     Programmable Synthetic Protein Circuits for the Identification and
            Suppression of Hepatocellular Carcinoma
  JOURNAL   Mol Ther Oncolytics 17, 70-82 (2020)
   PUBMED   32322664
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Liu K, Yang J, Ding S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2020) Second Department of Hepatobiliary Surgery,
            Zhujiang Hospital, No. 253, Industrial Avenue, Haizhu District,
            Guangzhou, Guangdong 510245, China
REFERENCE   3  (bases 1 to 6904)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther
            Oncolytics 17, 70-82 (2020)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2020) Second Department of Hepatobiliary Surgery, Zhujiang
            Hospital, No. 253, Industrial Avenue, Haizhu District, Guangzhou,
            Guangdong 510245, China"
FEATURES             Location/Qualifiers
     source          1..6904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             965..2437
                     /gene="STK3"
                     /label="Serine/threonine-protein kinase 3"
                     /note="Serine/threonine-protein kinase 3 from Homo sapiens.
                     Accession#: Q13188"
     polyA_signal    2504..2728
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2774..3202
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3216..3545
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3612..4403
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4580..4713
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4750..4766)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4774..4790)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4798..4828)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4843..4864)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5152..5737)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5911..6768)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6769..6873)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatggag cagccgccgg cgcctaagag taaactaaaa aagctgagtg aagacagttt
     1021 gactaagcag cctgaagaag tttttgatgt attagagaag cttggagaag ggtcttatgg
     1081 aagtgtattt aaagcaatac acaaggaatc cggtcaagtt gtcgcaatta aacaagtacc
     1141 tgttgaatca gatcttcagg aaataatcaa agaaatttcc ataatgcagc aatgtgacag
     1201 cccatatgtt gtaaagtact atggcagtta ttttaagaat acagacctct ggattgttat
     1261 ggagtactgt ggcgctggct ctgtctcaga cataattaga ttacgaaaca agacattaat
     1321 agaagatgaa attgcaacca ttcttaaatc tacattgaaa ggactagaat atttgcactt
     1381 tatgagaaaa atacacagag atataaaagc tggaaatatt ctcctcaata cagaaggaca
     1441 tgcaaaattg gcagattttg gagtggctgg tcagttaaca gatacaatgg caaaacgcaa
     1501 tactgtaata ggaactccat tttggatggc tcctgaggtg attcaagaaa taggctataa
     1561 ctgtgtggcc gacatctggt cccttggcat tacttctata gaaatggctg aaggaaaacc
     1621 tccttatgct gatatacatc caatgagggc tatttttatg attcccacaa atccaccacc
     1681 aacattcaga aagccagaac tttggtccga tgatttcacc gattttgtta aaaagtgttt
     1741 ggtgaagaat cctgagcaga gagctactgc aacacaactt ttacagcatc cttttatcaa
     1801 gaatgccaaa cctgtatcaa tattaagaga cctgatcaca gaagctatgg agatcaaagc
     1861 taaaagacat gaggaacagc aacgagaatt ggaagaggaa gaagaaaatt cggatgaaga
     1921 tgagctggat tcccacacca tggtgaagac tagtgtggag agtgtgggca ccatgcgggc
     1981 cacaagcacg atgagtgaag gggcccagac catgattgaa cataatagca cgatgttgga
     2041 atccgacttg gggaccatgg tgataaacag tgaggatgag gaagaagaag atggaactat
     2101 gaaaagaaat gcaacctcac cacaagtaca aagaccatct ttcatggact actttgataa
     2161 gcaagacttc aagaataaga gtcacgaaaa ctgtaatcag aacatgcatg aacccttccc
     2221 tatgtccaaa aacgtttttc ctgataactg gaaagttcct caagatggag actttgactt
     2281 tttgaaaaat ctaagtttag aagaactaca gatgcggtta aaagcactgg accccatgat
     2341 ggaacgggag atagaagaac ttcgtcagag atacactgcg aaaagacagc ccattctgga
     2401 tgcgatggat gcaaagaaaa gaaggcagca aaacttttga atccagcaca gtggcggccg
     2461 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     2761 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     2881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3121 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3181 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3241 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3301 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3361 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3421 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3481 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3541 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     3601 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3661 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3721 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3781 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3841 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3901 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3961 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4021 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4081 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4141 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4201 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4261 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4321 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4381 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4441 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4501 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4561 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4621 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4681 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     4741 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4801 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4861 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4921 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4981 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5041 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5101 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5161 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5221 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5281 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5341 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5401 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5461 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5521 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5581 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5641 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     5701 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5761 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5821 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5881 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5941 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6001 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6061 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6121 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6181 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6241 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6301 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6361 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6421 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6481 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6541 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6601 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6661 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6721 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6781 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6841 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6901 cgtc
//
