LOCUS       V015547                 8821 bp    DNA     circular SYN 26-MAY-2020
DEFINITION  Exported.
ACCESSION   V015547
VERSION     V015547
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Han X, Yang J, Zeng F, Weng J, Zhang Y, Peng Q, Shen L, Ding S, Liu
            K, Gao Y.
  TITLE     Programmable Synthetic Protein Circuits for the Identification and
            Suppression of Hepatocellular Carcinoma
  JOURNAL   Mol Ther Oncolytics 17, 70-82 (2020)
   PUBMED   32322664
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Liu K, Yang J, Ding S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2020) Second Department of Hepatobiliary Surgery,
            Zhujiang Hospital, No. 253, Industrial Avenue, Haizhu District,
            Guangzhou, Guangdong 510245, China
REFERENCE   3  (bases 1 to 8821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther
            Oncolytics 17, 70-82 (2020)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2020) Second Department of Hepatobiliary Surgery, Zhujiang
            Hospital, No. 253, Industrial Avenue, Haizhu District, Guangzhou,
            Guangdong 510245, China"
FEATURES             Location/Qualifiers
     source          1..8821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             965..4354
                     /gene="LATS1"
                     /label="Serine/threonine-protein kinase LATS1"
                     /note="Serine/threonine-protein kinase LATS1 from Homo
                     sapiens. Accession#: O95835"
     polyA_signal    4421..4645
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4691..5119
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5133..5462
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5529..6320
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6497..6630
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6667..6683)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6691..6707)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6715..6745)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6760..6781)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7069..7654)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7828..8685)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8686..8790)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatgaag aggagtgaaa agccagaagg atatagacaa atgaggccta agacctttcc
     1021 tgccagtaac tatactgtca gtagccggca aatgttacaa gaaattcggg aatcccttag
     1081 gaatttatct aaaccatctg atgctgctaa ggctgagcat aacatgagta aaatgtcaac
     1141 cgaagatcct cgacaagtca gaaatccacc caaatttggg acgcatcata aagccttgca
     1201 ggaaattcga aactctctgc ttccatttgc aaatgaaaca aattcttctc ggagtacttc
     1261 agaagttaat ccacaaatgc ttcaagactt gcaagctgct ggatttgatg aggatatggt
     1321 tatacaagct cttcagaaaa ctaacaacag aagtatagaa gcagcaattg aattcattag
     1381 taaaatgagt taccaagatc ctcgacgaga gcagatggct gcagcagctg ccagacctat
     1441 taatgccagc atgaaaccag ggaatgtgca gcaatcagtt aaccgcaaac agagctggaa
     1501 aggttctaaa gaatccttag ttcctcagag gcatggcccg ccactaggag aaagtgtggc
     1561 ctatcattct gagagtccca actcacagac agatgtagga agacctttgt ctggatctgg
     1621 tatatcagca tttgttcaag ctcaccctag caacggacag agagtgaacc ccccaccacc
     1681 acctcaagta aggagtgtta ctcctccacc acctccaaga ggccagactc cccctccaag
     1741 aggtacaact ccacctcccc cttcatggga accaaactct caaacaaagc gctattctgg
     1801 aaacatggaa tacgtaatct cccgaatctc tcctgtccca cctggggcat ggcaagaggg
     1861 ctatcctcca ccacctctca acacttcccc catgaatcct cctaatcaag gacagagagg
     1921 cattagttct gttcctgttg gcagacaacc aatcatcatg cagagttcta gcaaatttaa
     1981 ctttccatca gggagacctg gaatgcagaa tggtactgga caaactgatt tcatgataca
     2041 ccaaaatgtt gtccctgctg gcactgtgaa tcggcagcca ccacctccat atcctctgac
     2101 agcagctaat ggacaaagcc cttctgcttt acaaacaggg ggatctgctg ctccttcgtc
     2161 atatacaaat ggaagtattc ctcagtctat gatggtgcca aacagaaata gtcataacat
     2221 ggaactatat aacattagtg tacctggact gcaaacaaat tggcctcagt catcttctgc
     2281 tccagcccag tcatccccga gcagtgggca tgaaatccct acatggcaac ctaacatacc
     2341 agtgaggtca aattctttta ataacccatt aggaaataga gcaagtcact ctgctaattc
     2401 tcagccttct gctacaacag tcactgcaat tacaccagct cctattcaac agcctgtgaa
     2461 aagtatgcgt gtattaaaac cagagctaca gactgcttta gcacctacac acccttcttg
     2521 gataccacag ccaattcaaa ctgttcaacc cagtcctttt cctgagggaa ccgcttcaaa
     2581 tgtgactgtg atgccacctg ttgctgaagc tccaaactat caaggaccac caccacccta
     2641 cccaaaacat ctgctgcacc aaaacccatc tgttcctcca tacgagtcaa tcagtaagcc
     2701 tagcaaagag gatcagccaa gcttgcccaa ggaagatgag agtgaaaaga gttatgaaaa
     2761 tgttgatagt ggggataaag aaaagaaaca gattacaact tcacctatta ctgttaggaa
     2821 aaacaagaaa gatgaagagc gaagggaatc tcgtattcaa agttattctc ctcaagcatt
     2881 taaattcttt atggagcaac atgtagaaaa tgtactcaaa tctcatcagc agcgtctaca
     2941 tcgtaaaaaa caattagaga atgaaatgat gcgggttgga ttatctcaag atgcccagga
     3001 tcaaatgaga aagatgcttt gccaaaaaga atctaattac atccgtctta aaagggctaa
     3061 aatggacaag tctatgtttg tgaagataaa gacactagga ataggagcat ttggtgaagt
     3121 ctgtctagca agaaaagtag atactaaggc tttgtatgca acaaaaactc ttcgaaagaa
     3181 agatgttctt cttcgaaatc aagtcgctca tgttaaggct gagagagata tcctggctga
     3241 agctgacaat gaatgggtag ttcgtctata ttattcattc caagataagg acaatttata
     3301 ctttgtaatg gactacattc ctgggggtga tatgatgagc ctattaatta gaatgggcat
     3361 ctttccagaa agtctggcac gattctacat agcagaactt acctgtgcag ttgaaagtgt
     3421 tcataaaatg ggttttattc atagagatat taaacctgat aatattttga ttgatcgtga
     3481 tggtcatatt aaattgactg actttggcct ctgcactggc ttcagatgga cacacgattc
     3541 taagtactat cagagtggtg accatccacg gcaagatagc atggatttca gtaatgaatg
     3601 gggggatccc tcaagctgtc gatgtggaga cagactgaag ccattagagc ggagagctgc
     3661 acgccagcac cagcgatgtc tagcacattc tttggttggg actcccaatt atattgcacc
     3721 tgaagtgttg ctacgaacag gatacacaca gttgtgtgat tggtggagtg ttggtgttat
     3781 tctttttgaa atgttggtgg gacaacctcc tttcttggca caaacaccat tagaaacaca
     3841 aatgaaggtt atcaactggc aaacatctct tcacattcca ccacaagcta aactcagtcc
     3901 tgaagcttct gatcttatta ttaaactttg ccgaggaccc gaagatcgct taggcaagaa
     3961 tggtgctgat gaaataaaag ctcatccatt ttttaaaaca attgacttct ccagtgacct
     4021 gagacagcag tctgcttcat acattcctaa aatcacacac ccaacagata catcaaattt
     4081 tgatcctgtt gatcctgata aattatggag tgatgataac gaggaagaaa atgtaaatga
     4141 cactctcaat ggatggtata aaaatggaaa gcatcctgaa catgcattct atgaatttac
     4201 cttccgaagg ttttttgatg acaatggcta cccatataat tatccgaagc ctattgaata
     4261 tgaatacatt aattcacaag gctcagagca gcagtcggat gaagatgatc aaaacacagg
     4321 ctcagagatt aaaaatcgcg atctagtata tgtttaaatc cagcacagtg gcggccgctc
     4381 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     4441 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4501 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4561 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4621 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     4681 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4741 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4801 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4861 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4921 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4981 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5041 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5101 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     5161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     5221 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5281 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     5341 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     5401 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     5461 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     5521 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5581 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5641 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5701 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5761 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5821 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5881 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5941 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6001 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     6061 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6121 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6181 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6241 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6301 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6361 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6421 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6481 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6601 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     6661 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6721 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6781 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa
     7621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8821 c
//
