LOCUS       V015486                 8234 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V015486
VERSION     V015486
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1318..3846
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype H (isolate
                     United States/LAS2523/2002). Accession#: Q8JMY7"
     polyA_signal    4247..4471
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4517..4945
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4959..5289
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5337..5384
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5403..5774
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5907..6040
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6077..6093)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6101..6117)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6125..6155)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6170..6191)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6479..7067)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7241..8098)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8099..8203)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg caccatgcaa cttttccacc
      841 tctgcctaat catcttttgt tcatgtccca ctgttcaagc ctccaagctg tgccttgggt
      901 ggctttgggg catggacatt gacccttata aagaatttgg agcttctgtg gagttactct
      961 catttttgcc ttctgacttc ttcccgtctg tccgggacct actcaacacc gcttcagccc
     1021 tctaccgaga tgccttagaa tcaccagaac attgcacccc cagccacact gctctcaggc
     1081 aagctatttt gtgctggggt gagttgatga ccttggcttc ctgggtgggc aataatttag
     1141 aggatcctgg agcaagagat ctagtagtta attatgtcaa tactaacatg ggcctaaaaa
     1201 ttagacaatt attatggttt cacatttctt gccttacatt tggaagagaa actgtgcttg
     1261 agtatttggt gtcttttgga gtgtggattc gcactccacc tgcttataga ccaccaaatg
     1321 cccctatcct gtcaacactt ccggagactt gtgttgttag acaacgaggc agggccccta
     1381 gaagaagaac tccctcgcct cgcagacgaa gatctcaatc tccgcgtcgc agaagatctc
     1441 aatctccagc ttcccaatgt tagtattcct tggactcata aggtgggaaa ctttaccggt
     1501 ctttactcct ctactatacc tgttttcaat cctgactggt taactccttc ttttcctgac
     1561 attcacttgc atcaagattt gatacaaaaa tgtgaacaat ttgtaggccc actcactact
     1621 aatgaaagga gacgattgaa actaattatg ccagctaggt tttatcccaa agttactaaa
     1681 tacttccctt tggataaagg tattaagcct tactatccag agaatgtggt taatcattac
     1741 tttaaaacta gacattattt acatactttg tggaaggcag gaattctata taagagagaa
     1801 tccacacata gcgcctcatt ttgtgggtca ccatattcct gggaacaaga gctacagcat
     1861 gggagcacct ctctcaacgg cgagaagggg catgggacag aatctttctg tgcccaatcc
     1921 tctgggattc tttccagacc accagttgga tccactattc agagcaaatt ccagcagtcc
     1981 cgattgggac ttcaacacaa acaaggacaa ttggccaatg gcaaacaagg taggagtggg
     2041 aggcttcggt ccagggttca cacccccaca cggtggcctt ctggggtgga gccctcaggc
     2101 acagggcatt ctaacaacct cgccaccaga tccacctcct gcttccacca atcggaggtc
     2161 aggaagaaag ccaaccccag tctctccacc tctaagggac acacatccac aggccatgca
     2221 gtggaactca acacagttcc accaagcact gttggatccg agagtaaggg ggctgtatct
     2281 tcctgctggt ggctccagtt cagaaacaca gaaccctgct ccgactattg cctctctcac
     2341 atcatcaatc ttctcgaaga ctggggaccc tgctatgaac atggagaaca tcacatcagg
     2401 actcctagga ccccttctcg tgttacaggc ggtgtgtttc ttgttgacaa aaatcctcac
     2461 aataccaaag agtctagact cgtggtggac ttctctcaat tttctagggg taccacccgg
     2521 gtgtcctggc caaaattcgc agtccccaac ctccaatcac ttaccaacct cctgtcctcc
     2581 aacttgtcct ggctatcgtt ggatgtgtct gcggcgtttt atcatcttcc tcttcatcct
     2641 gctgctatgc ctcatcttct tgttggttct tctggactat caaggtatgt tgcccgtgtg
     2701 tcctctactt ccaggatcta caaccaccag cacgggaccc tgcaaaacct gcaccactct
     2761 tgctcaagga acctctatgt ttccctcctg ctgctgtacc aaaccttcgg acggaaattg
     2821 cacctgtatt cccatcccat catcttgggc tttcggaaaa tacctatggg agtgggcctc
     2881 agcccgtttc tcttggctca gtttactagt gcaatttgtt cagtggtgcg tagggctttc
     2941 ccccactgtc tggcttttag ttatatggat gatctggtat tgggggccaa atctgtgcag
     3001 catcttgagt ccctttatac cgctgttacc aattttttgt tatctgtggg catccatttg
     3061 aacacagcta aaacaaaatg gtggggttat acccttcact ttatgggtta tagaattggg
     3121 agttggggga ccttgcctca ggaacatatt gtgcataaaa tcaaagattg ctttcgcaaa
     3181 cttcccgtga atagacccat tgattggaag gtttgtcaac gcattgtagg tcttttgggc
     3241 tttgcagccc cttttactca atgtggttat cctgctctca tgcccttgta tgcctgtatt
     3301 accgctaagc aggcttttgt tttctcgcct acttacaagg cctttctctg tcaacaatac
     3361 atgaaccttt accccgttgc tcggcaacgg ccaggccttt gccaagtgtt tgctgacgca
     3421 acccccactg gctggggctt ggcgattggc catcagcgca tgcgcggaac ctttgtggct
     3481 cctctgccga tccatactgc ggaactccta gccgcttgtt tcgctcgcag caggtctgga
     3541 gcggacatta tcggcactga caactccgtt gtcctttctc ggaagtacac ctccttccca
     3601 tggccgctag gatgtgctgc caactggatc ctgcgcggga cgtcctttgt ctacgtcccg
     3661 tcggcgctga atcctgcgga cgacccctct cgtggtcgct tggggctctg ccgccctctt
     3721 ctccgcctgc cgttccggcc gacgacgggt cgcacctctc tttacgcgga ctccccgcct
     3781 gtgccttctc atctgccggc ccgtgtgcac ttcgcttcac ctctgcacgt cgcatggaga
     3841 ccaccgtgaa cgccccttgg aacttgccaa caaccttaca taagaggact cttggacttt
     3901 cgccccggtc aacgacctgg attgaggaat acatcaaaga ctgtgtattt aaggactggg
     3961 aggagtcggg ggaggagttg aggttaatga tctttgtatt aggaggctgt aggcataaat
     4021 tggtctgttc accagcacca tgcaactttt tcacctctgc ctaatcatct tttgttcatg
     4081 tcccactgtt caagcctcca agctgtgcct tgggtggctt tggggcatgg acattgaccc
     4141 ttataaagaa tttggagctt ctgtggagtt actctcattt ttgccttctg acttcttccc
     4201 gtctgtccgg gaccaagctt aagtttaaac cgctgatcag cctcgactgt gccttctagt
     4261 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4321 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4381 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4441 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4501 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4561 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4621 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct
     4681 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4741 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4801 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4861 tattcttttg atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg
     4921 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4981 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5041 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5101 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5161 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5221 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5281 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     5341 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     5401 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     5461 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     5521 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     5581 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     5641 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     5701 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     5761 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     5821 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5881 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     5941 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6001 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     6061 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6121 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     6181 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6241 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6301 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6361 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6661 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6901 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7141 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7201 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7261 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7321 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7381 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7441 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7501 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7561 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7621 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7681 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7741 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7801 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7861 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     7921 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     7981 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8041 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8101 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8161 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8221 tgccacctga cgtc
//
