LOCUS       V015612                 8309 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V015612
VERSION     V015612
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1360..3885
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype G (isolate
                     United States/USG17/2002). Accession#: Q8QZQ2"
     polyA_signal    4322..4546
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4592..5020
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5034..5364
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5412..5459
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5478..5849
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5982..6115
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6152..6168)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6176..6192)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6200..6230)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6245..6266)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6554..7142)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7316..8173)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8174..8278)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgtaacttt
      841 ttcacctctg cctaatcatc tcttgttcat gtcctactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttagggcatg gatagaacaa ctttgccata tggccttttt ggcttagaca
      961 ttgaccctta taaagaattt ggagctactg tggagttgct ctcgtttttg ccttctgact
     1021 ttttcccgtc tgttcgtgat cttctcgaca ccgcttcagc tttgtaccgg gaatccttag
     1081 agtcctctga tcattgttcg cctcaccata cagcactcag gcaagcaatc ctgtgctggg
     1141 gtgagttgat gactctagct acctgggtgg gtaataattt ggaagatcca gcatccagag
     1201 atttggtggt caattatgtt aatactaata tgggtttaaa aatcaggcaa ctattgtggt
     1261 ttcacatttc ctgtcttact tttgggagag aaaccgttct tgagtatttg gtgtcttttg
     1321 gagtgtggat tcgcactcct cctgcttata gaccaccaaa tgcccctatc ctatcaacac
     1381 ttccggagac tactgttgct agacgaagag gcaggtcccc tcgaagaaga actccctcgc
     1441 ctcgcagacg aagatctcaa tcgccgcgtc gcagaagatc tgcatctcca gcttcccaat
     1501 gttagtattc cttggactca caaggtggga aactttacgg ggctgtattc ttctactata
     1561 cctgtcttta atcctgattg gcaaactcct tcttttccaa atatccattt gcatcaaggc
     1621 attataacta aatgtgaaca atttgtgggc cctctcacag taaatgagaa acgaagatta
     1681 aaactagtta tgcctgccag atttttccca aactctacta aatatttacc attagacaaa
     1741 ggtatcaaac cgtattatcc agaaaatgta gttaatcatt acttccagac cagacattat
     1801 ttacataccc tttggaaggc gggtattcta tataagagag aaacatcccg tagcgcttca
     1861 ttttgtgggt caccatatac ttgggaacaa gatctacagc atggggcttt cttggacggt
     1921 ccctctcgag tggggaaaga acctttccac cagcaatcct ctaggattcc ttcccgatca
     1981 ccagttggac ccagcattca gagcaaatac caacaatcca gattgggact tcaatcccaa
     2041 aaaggaccct tggccagagg ccaacaaggt aggagttgga gcctatggac ccgggttcac
     2101 ccctccacac ggaggccttt tggggtggag ccctcagtct cagggcacac taacaacttt
     2161 gccagcagat ccgcctcctg cctccaccaa tcgtcagtca gggaggcagc ctactcccat
     2221 ctctccacca ctaagagaca gtcatcctca ggccatgcag tggaactcta cagcattcca
     2281 ccaagctcta caaaatccca aagtcagggg cctgtatttt cctgctggtg gctccagttc
     2341 agggatagtg aaccctgttc cgactattgc ctctcacatc tcgtcaatct tctccaggat
     2401 tggggaccct gcaccgaaca tggagaacat cacatcagga ttcctaggac ccctgctcgt
     2461 gttacaggcg gggtttttct tgttgacaag aatcctcaca ataccacaga gtctagactc
     2521 gtggtggact tctctcaatt ttctaggggg agtgcccgtg tgtcctggcc taaattcgca
     2581 gtccccaacc tccaatcact caccaatctc ctgtcctcca acttgtcctg gctatcgctg
     2641 gatgtgtctg cggcgtttta tcatattcct cttcatcctg ctgctatgcc tcatcttctt
     2701 gttggttctt ctggactatc aaggtatgtt gcccgtttgt cctctgattc caggatcctc
     2761 gaccaccagt acgggaccct gcaaaacctg cacgactcct gctcaaggca actctatgta
     2821 tccctcatgt tgctgtacaa aaccttcgga cggaaattgc acctgtattc ccatcccatc
     2881 atcttgggct ttcgcaaaat acctatggga gtgggcctca gtccgtttct cttggctcag
     2941 tttactagtg ccatttgttc agtggttcgt agggctttcc cccactgtct ggctttcagc
     3001 tatatggatg atgtggtatt gggggccaaa tctgtacaac atcttgagtc cctttatacc
     3061 gctgttacca attttctttt gtctttgggt atacatctaa accctaccaa aacaaaaaga
     3121 tggggttatt ccttaaattt tatgggatat gtaattggaa gttggggtac tttgccacaa
     3181 gaacacatca cacagaaaat taagcaatgt tttcggaaac tccctgttaa caggccaatt
     3241 gattggaaag tctgtcaacg aataactggt ctgttgggtt tcgctgctcc ttttacccaa
     3301 tgtggttacc ctgccttaat gcctttatat gcatgtatac aagctaagca ggcttttact
     3361 ttctcgccaa cttataaggc ctttctctgt aaacaataca tgaaccttta ccccgttgct
     3421 aggcaacggc ccggtctgtg ccaagtgttt gctgacgcaa cccccactgg ttggggcttg
     3481 gccatcggcc atcagcgcat gcgtggaacc tttgtggctc ctctgccgat ccatactgcg
     3541 gaactcctag ctgcttgttt tgctcgcagc cggtctggag caaaactcat tgggactgac
     3601 aattctgtcg tcctttctcg gaaatataca tcctttccat ggctgctagg ctgtgctgcc
     3661 aactggatcc ttcgcgggac gtcctttgtt tacgtcccgt cagcgctgaa tccagcggac
     3721 gacccctccc ggggccgttt ggggctctgt cgcccccttc tccgtctgcc gttcctgccg
     3781 accacggggc gcacctctct ttacgcggtc tccccgtctg tgccttctca tctgccggac
     3841 cgtgtgcact tcgcttcacc tctgcacgtt acatggaaac cgccatgaac acctctcatc
     3901 atctgccaag gcagttatat aagaggactc ttggactgtt tgttatgtca acaaccgggg
     3961 tggagaaata cttcaaggac tgtgtttttg ctgagtggga agaattaggc aatgagtcca
     4021 ggttaatgac ctttgtatta ggaggctgta ggcataaatt ggtctgcgca ccagcaccat
     4081 gtaacttttt cacctctgcc taatcatctc ttgttcatgt cctactgttc aagcctccaa
     4141 gctgtgcctt gggtggcttt agggcatgga tagaacaact ttgccatatg gcctttttgg
     4201 cttagacatt gacccttata aagaatttgg agctactgtg gagttgctct cgtttttgcc
     4261 ttctgacttt ttcccgtctg ttcgtgatca agcttaagtt taaaccgctg atcagcctcg
     4321 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4381 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4441 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4501 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4561 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4621 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4681 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4741 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4801 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4861 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4921 aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg
     4981 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5041 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
     5101 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5161 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5281 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5341 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5401 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     5461 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     5521 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     5581 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     5641 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     5701 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     5761 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     5821 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     5881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6121 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6721 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6961 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8281 catttccccg aaaagtgcca cctgacgtc
//
