LOCUS       V015643                 8240 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V015643
VERSION     V015643
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1324..3852
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype F2
                     (isolate Brazil/w4B). Accession#: Q05486"
     polyA_signal    4253..4477
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4523..4951
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4965..5295
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5343..5390
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5409..5780
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5913..6046
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6099)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6107..6123)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6131..6161)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6176..6197)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6485..7073)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7247..8104)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8105..8209)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc ttttgttcat gtcccactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacattgacc cttataaaga atttggagct tctgtggagt
      961 tactctcgtt tttgccttct gatttcttcc catcggttcg ggacctactc gacaccgctt
     1021 cagctcttta ccgggatgct ttagagtcac ctgaacattg cactcccaac catactgctc
     1081 tcaggcaagc tattttgtgt tggggtgagt taatgacttt ggcttcctgg gtgggcaata
     1141 atttggagga ccctgcagct agggatttag tagttaacta tgttaacact aacatgggcc
     1201 taaaaattag acaactgttg tggtttcaca tttcctgcct tacttttgga agagaaacag
     1261 ttctagagta tttggtgtcc tttggagtgt ggattcgcac tcctcctgct tacagaccac
     1321 caaatgcccc tatcctatcc acacttccgg aaactactgt tgttagacga cgaggcaggt
     1381 cccctagaag aagaactccc tcgcctcgca gacgaagatc tcaatcgccg cgtcgccgca
     1441 gatctcaatc tccagcttcc caatgttagt attccttgga ctcataaggt gggaaacttt
     1501 acggggcttt actcttctac tgtgcctgct tttaatccta actggtccac tccttctttt
     1561 cctgatattc atttgcatca agacctgatt tctaaatgtg aacaatttgt aggcccactt
     1621 actaaaaatg aattacgaag attaaaattg gttatgccag ctagatttta tcctaaggtt
     1681 accaaatatt ttcccatgga taaaggcatc aaaccctatt atcctgagca tgcagttaat
     1741 cattacttta aaaccagaca ttatttgcat actttatgga aggcgggagt tttatataag
     1801 agagaatcca cacgtagcgc ctcattttgt gggtcaccat attcctggga acaagagcta
     1861 cagcatggga gcacctctct caacgacacg aagaggcatg ggacagaatc tctctgtgcc
     1921 caatcctctg ggattctttc cagaccatca gctggatccg ctattcagag caaattccag
     1981 cagtcccgac tgggacttca acacaaacaa ggacagttgg ccaatggcaa acaaggtagg
     2041 agtgggaggc tacggtccag ggttcacacc cccacacggt ggcctgctgg ggtggagccc
     2101 tcaagcacaa ggtgtgttaa caaccttgcc agcagatccg cctcctgctt ccaccaatcg
     2161 gcggtccggg agaaagccaa ccccagtctc tccacctcta agagacacac atccacaggc
     2221 aatgcagtgg aactcaaccc agttccacca agctctgttg gatcccaggg taagggctct
     2281 gtacttccct gctggtggct ccagttcagg gacacagaac cctgctccga ctattgcctc
     2341 tctcacatca tcaatcttct cgaagactgg gggccctgct atgaacatgg acaacattac
     2401 atcaggactc ctaggacccc tgctcgtgtt acaggcggtg tgtttcttgt tgacaaaaat
     2461 cctcacaata ccacagagtc tagactcgtg gtggacttct ctcaattttc tagggggact
     2521 acccgggtgt cctggccaaa attcgcagtc cccaacctcc aatcacttac caacctcctg
     2581 tcctccaact tgtcctggct atcgatggat gtgtctgcgg cgttttatca tcttcctctt
     2641 catcctgctg ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc
     2701 cgtttgtcct ctacttccag gatccacgac caccagcacg ggaccctgca aaacctgcac
     2761 aactcttgca caaggaacct ctatgtttcc ctcctgttgc tgttccaaac cctcggacgg
     2821 aaactgcact tgtattccca tcccatcatc ctgggcttta ggaaaatacc tatgggagtg
     2881 ggcctcagcc cgtttctcct ggctcagttt actagtgcaa tttgttcagt ggtgcgtcgg
     2941 gctttccccc actgtttggc ttttagttat atggatgatc tggtattggg ggccaaatct
     3001 gtgcagcatc ttgagtccct ttataccgct gttaccaatt ttctgttatc tgtgggtatc
     3061 catttaaata cctctaaaac aaaaagatgg ggttactccc tacattttat gggttatgtc
     3121 attggtagtt ggggatcatt accccaagat cacattgtac acaaaatcaa ggaatgcttt
     3181 cgaaaactgc ctgtaaatcg tccaattgat tggaaagttt gtcaacgcat agtgggtctt
     3241 ttgggctttg ctgccccttt cacccaatgc ggttatcctg ctctcatgcc tctgtatgcc
     3301 tgtattactg ctaaacaggc ttttgtcttc tcgccaacct acaaggcctt tctgtgtaaa
     3361 caatacatga acctttaccc cgttgctcgg caacggccag gcctgtgcca agtgtttgct
     3421 gacgcaaccc ccactggttg gggcttggcc attggccatc agcgcatgcg tggaaccttt
     3481 gtggctcctc tgccgatcca tactgcggaa ctcctagcag cttgtttcgc tcgcagcagg
     3541 tctggagcga ctctcatcgg cacggacaat tctgttgtcc tctctaggaa gtacacctcc
     3601 tttccatggc tgctcggatg tgctgcaaac tggatcctgc gcgggacgtc ctttgtttac
     3661 gtcccatcgg cgctgaatcc cgcggacgac ccctcccggg gccgcttggg gctgtaccgc
     3721 cctcttctcc gtctgccgtt ccagccgacg acgggtcgca cctctcttta cgcggactcc
     3781 ccgtctgttc cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca
     3841 tggagaccac cgtgaacgcc ccctggagtt tgccaacagt cttacataag cggactcttg
     3901 gactttcagg atggtcaatg acctggatcg aagaatacat caaagactgt gtatttaagg
     3961 actgggagga gttgggggag gagattaggt taaaggtctt tgtattaggg ggctgtaggc
     4021 ataaattggt ctgttcacca gcaccatgca actttttcac ctctgcctaa tcatcttttg
     4081 ttcatgtccc actgttcaag cctccaagct gtgccttggg tggctttggg gcatggacat
     4141 tgacccttat aaagaatttg gagcttctgt ggagttactc tcgtttttgc cttctgattt
     4201 cttcccatcg gttcgggacc aagcttaagt ttaaaccgct gatcagcctc gactgtgcct
     4261 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4321 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4381 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4441 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4501 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     4681 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4861 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     4921 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4981 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     5041 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5101 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5161 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5221 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5281 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5341 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5401 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5461 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     5521 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     5581 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     5641 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     5701 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     5761 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     5821 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5881 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6061 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8221 gaaaagtgcc acctgacgtc
//
