LOCUS       V016388                 8237 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V016388
VERSION     V016388
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1324..3849
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype E (isolate
                     Cote d'Ivoire/ABI-129/2003). Accession#: Q80IU7"
     polyA_signal    4250..4474
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4520..4948
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4962..5292
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5340..5387
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5406..5777
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5910..6043
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6080..6096)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6104..6120)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6128..6158)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6173..6194)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7244..8101)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8102..8206)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcttgttcat gtcctactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacattgacc cttataaaga atttggagct actgtggagt
      961 tactctcgtt tttgccttct gacttctttc cttcagtaag agatcttcta gataccgcct
     1021 cagctctgta tcgggatgcc ttagaatctc ctgagcattg ttcaccgcac cacactgcac
     1081 tcaggcaagc cattctttgc tggggggaac taatgactct agctacctgg gtgggtgtaa
     1141 atttggaaga tccagcatcc agggacctag tagtcagtta tgtcaatact aatatgggcc
     1201 taaagttcag gcaattattg tggtttcaca tttcttgtct cacttttgga agagaaaccg
     1261 tcatagagta tttggtgtct tttggagtgt ggattcgcac tcctccagct tatagaccac
     1321 caaatgcccc tatcttatca acacttccgg agaatactgt tgttagacga agaggcaggt
     1381 cccctagaag aagaactccc tcgcctcgca gacgaagatc tcaatcgccg cgtcgcagaa
     1441 gatctcaatc tccagcttcc caatgttagt attccttgga ctcacaaggt gggaaatttt
     1501 acggggcttt actcttctac tatacctgtc tttaatccta actggaaaac tccatctttt
     1561 cctgatattc atttgcacca ggacattatt aacaaatgtg aacaatttgt aggtcctcta
     1621 acagtaaatg aaaaacgaag attaaaatta gtcatgcctg ctagattttt tcccatctct
     1681 acgaaatatt tgcccctaga gaaaggtata aaaccttatt atcccgataa tgtagttaat
     1741 cattacttcc aaaccagaca ctatttacat accctatgga aggcgggcat cttatataaa
     1801 agagaaacta cacgtagcgc ctcattttgt gggtcacctt attcttggga acaagagcta
     1861 catcatgggg ctttcttgga cggtccctct cgaatggggg aagaatcatt ccaccaccaa
     1921 tcctctggga ttttttcccg accaccagtt ggatccagca ttcagagcaa acaccagaaa
     1981 tccagattgg gaccacaatc ccaacaaaga ccactggacg gaagccaaca aggtaggagt
     2041 gggagcattc gggccggggt tcactccccc acacggaggc cttttggggt ggagccctca
     2101 ggctcaaggc atgctaaaaa cattgccagc agatccgcct cctgcctcca ccaatcggca
     2161 gtcaggaagg cagcctaccc caatcactcc acctttgaga gacactcatc ctcaggccat
     2221 gcagtggaat tccacaacat tccaccaagc tctgcaggat cccagagtaa gaggcctgta
     2281 ttttcctgct ggtggctcca gttccggaac agtgaaccct gttccgacta ctgcctcact
     2341 catctcgtca atcttctcga ggattgggga ccctgcaccg aacatggaaa gcatcacatc
     2401 aggattccta ggacccctgc tcgtgttaca ggcggggttt ttcttgttga caaaaatcct
     2461 cacaataccg cagagtctag actcgtggtg gacttctctc aattttctag ggggagctcc
     2521 cgtgtgtctt ggccaaaatt cgcagtcccc aacctccaat cactcaccaa cctcttgtcc
     2581 tccaatttgt cctggctatc gctggatgtg tctgcggcgt tttatcatct tcctcttcat
     2641 cctgctacta tgcctcatct tcttgttggt tcttctggac tatcaaggta tgttgcccgt
     2701 ttgtcctcta attccaggat catcaaccac cagtacggga ccctgccgaa cctgcacgac
     2761 tcttgctcaa ggaacctcta tgtttccctc atgttgctgt tcaaaacctt cggacggaaa
     2821 ttgcacttgt attcccatcc catcatcatg ggctttcgga aaattcctat gggagtgggc
     2881 ctcagcccgt ttctcctggc tcagtttact agtgccattt gttcagtggt tcgccgggct
     2941 ttcccccact gtctggcttt cagttatatg gatgatgtgg tattgggggc caagtctgta
     3001 caacatcttg agtcccttta tacctctgtt accaattttc ttttgtcttt gggtatacat
     3061 ttaaatccca acaaaacaaa aagatgggga tattccctaa atttcatggg ttatgtaatt
     3121 ggaagttggg ggtcattacc acaggaacac atcagaatga aaatcaaaga ctgttttagg
     3181 aaactccctg ttaatcggcc tattgattgg aaagtatgtc aacgaattgt gggtcttttg
     3241 ggctttgctg ccccttttac acaatgtggg tatcctgctt taatgcctct gtatgcgtgt
     3301 attcactcta agcaggcttt cactttctcg ccaacttaca aggcctttct gtgtaaacaa
     3361 tacctgaccc tttaccccgt tgcccggcaa cggccaggtc tgtgccaagt gtttgctgat
     3421 gcaaccccca ctggctgggg cttggccata ggccatcagc gcatgcgcgg aacctttgtg
     3481 gctcctctgc cgatccatac tgcggaactc ctagccgctt gttttgctcg cagcaggtct
     3541 ggagcgaaac ttatcgggac agataattct gtcgttctct cccggaaata tacatccttt
     3601 ccatggctgc taggctgtgc tgccaactgg atcctgcgag ggacgtcctt tgtctacgtc
     3661 ccgtcagcgc tgaatcctgc ggacgaccca tctcggggtc gcttggggat ctatcgtccc
     3721 cttctccgtc tgccgttccg gccgaccacg gggcgcacct ctctttacgc ggtctccccg
     3781 tctgtgcctt ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca cgtcgcatgg
     3841 agaccaccgt gaacgcccac caaatcttgc ccaaggtctt acataagagg actcttggac
     3901 tctctgcaat gtcaacgacc gaccttgagg catacttcaa agactgtgtg tttaaagact
     3961 gggaggagtt gggggaggag attagattaa aggtctttgt actaggaggc tgtaggcata
     4021 aattggtctg cgcaccagca ccatgcaact ttttcacctc tgcctaatca tctcttgttc
     4081 atgtcctact gttcaagcct ccaagctgtg ccttgggtgg ctttggggca tggacattga
     4141 cccttataaa gaatttggag ctactgtgga gttactctcg tttttgcctt ctgacttctt
     4201 tccttcagta agagatcaag cttaagttta aaccgctgat cagcctcgac tgtgccttct
     4261 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4321 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4381 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4441 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     4501 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     4681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4861 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     4921 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     4981 gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca
     5041 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5101 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     5161 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     5221 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     5281 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     5341 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     5401 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     5461 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     5521 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     5581 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     5641 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     5701 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     5761 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     5821 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5881 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     5941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6061 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8221 aagtgccacc tgacgtc
//
