LOCUS       62056_6110        8226 bp DNA     circular SYN 21-DEC-2019
DEFINITION  Cloning vector pcDNA3.1Zeo(-)-HBV1.1- D, complete sequence.
ACCESSION   MN645906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of 
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg 
            69120, Deutschland
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2019) Molecular Virology, Department of Infectiology, 
            Heidelberg University Hospital, Inf344, Heidelberg 69120, 
            Deutschland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             918..1469
                     /codon_start=1
                     /transl_table=11
                     /product="core protein"
                     /label=core protein
                     /protein_id="QGX02070.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGVNLEDPASRDLVVSYVNTNMGLKFRQLLWFH
                     ISCLTFGRETVIEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRTPSP
                     RRRRSQSPRRRRSQSRESQC"
     CDS             2189..3031
                     /label=HBsAg (pre-S2/S)
                     /note="middle (pre-S2/S) envelope protein of hepatitis B
                     virus"
     CDS             3573..4037
                     /codon_start=1
                     /transl_table=11
                     /product="X protein"
                     /label=X protein
                     /protein_id="QGX02072.1"
                     /translation="MAARLCCQLDPARDVLCLRPVGAESCGRPFSGSLGTLSSPSPSAV
                     PTDHGAHLSLRGLPVCAFSSAGPCALRFTSARRMETTVNAHQILPKVLHKRTLGLSAMS
                     TTDLEAYFKDCLFKDWEELGEEIRLKVFVLGGCRHKLVCAPAPCNFFTSA"
     polyA_signal    4239..4463
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4509..4937
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4951..5281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        5329..5376
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             5395..5766
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    5899..6032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6069..6085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6093..6109)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6117..6147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6162..6183)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6471..7059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7233..8090)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8091..8195)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcttgttcat gtcctactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacatcgacc cttataaaga atttggagct actgtggagt
      961 tactctcgtt tttgccttct gacttctttc cttcagtacg agatcttcta gataccgcct
     1021 cagctctgta tcgggaagcc ttagagtctc ctgagcattg ttcacctcac catactgcac
     1081 tcaggcaagc aattctttgc tggggggaac taatgactct agctacctgg gtgggtgtta
     1141 atttggaaga tccagcgtct agagacctag tagtcagtta tgtcaacact aatatgggcc
     1201 taaagttcag gcaactcttg tggtttcaca tttcttgtct cacttttgga agagaaacag
     1261 ttatagagta tttggtgtct ttcggagtgt ggattcgcac tcctccagct tatagaccac
     1321 caaatgcccc tatcctatca acacttccgg agactactgt tgttagacga cgaggcaggt
     1381 cccctagaag aagaactccc tcgcctcgca gacgaaggtc tcaatcgccg cgtcgcagaa
     1441 gatctcaatc tcgggaatct caatgttagt attccttgga ctcataaggt ggggaacttt
     1501 actgggcttt attcttctac tgtacctgtc tttaatcctc attggaaaac accatctttt
     1561 cctaatatac atttacacca agacattatc aaaaaatgtg aacagtttgt aggcccactc
     1621 acagttaatg agaaaagaag attgcaattg attatgcctg ccaggtttta tccaaaggtt
     1681 accaaatatt taccattgga taagggtatt aaaccttatt atccagaaca tctagttaat
     1741 cattacttcc aaactagaca ctatttacac actctatgga aggcgggtat attatataag
     1801 agagaaacaa cacatagcgc ctcattttgt gggtcaccat attcttggga acaagatcta
     1861 cagcatgggg cagaatcttt ccaccagcaa tcctctggga ttctttcccg accaccagtt
     1921 ggatccagcc ttcagagcaa acaccgcaaa tccagattgg gacttcaatc ccaacaagga
     1981 cacctggcca gacgccaaca aggtaggagc tggagcattc gggctgggtt tcaccccacc
     2041 gcacggaggc cttttggggt ggagccctca ggctcagggc atactacaaa ctttgccagc
     2101 aaatccgcct cctgcctcca ccaatcgcca gtcaggaagg cagcctaccc cgctgtctcc
     2161 acctttgaga aacactcatc ctcaggccat gcagtggaat tccacaacct tccaccaaac
     2221 tctgcaagat cccagagtga gaggcctgta tttccctgct ggtggctcca gttcaggaac
     2281 agtaaaccct gttctgacta ctgcctctcc cttatcgtca atcttctcga ggattgggga
     2341 ccctgcgctg aacatggaga acatcacatc aggattccta ggaccccttc tcgtgttaca
     2401 ggcggggttt ttcttgttga caagaatcct cacaataccg cagagtctag actcgtggtg
     2461 gacttctctc aattttctag ggggaactac cgtgtgtctt ggccaaaatt cgcagtcccc
     2521 aacctccaat cactcaccaa cctcttgtcc tccaacttgt cctggttatc gctggatgtg
     2581 tctgcggcgt tttatcatct tcctcttcat cctgctgcta tgcctcatct tcttgttggt
     2641 tcttctggac tatcaaggta tgttgcccgt ttgtcctcta attccaggat cctcaacaac
     2701 cagcacggga ccatgccgga cctgcatgac tactgctcaa ggaacctcta tgtatccctc
     2761 ctgttgctgt accaaacctt cggacggaaa ttgcacctgt attcccatcc catcatcctg
     2821 ggctttcgga aaattcctat gggagtgggc ctcagcccgt ttctcctggc tcagtttact
     2881 agtgccattt gttcagtggt tcgtagggct ttcccccact gtttggcttt cagttatatg
     2941 gatgatgtgg tattgggggc caagtctgta cagcatcttg agtccctttt taccgctgtt
     3001 accaattttc ttttgtcttt gggtatacat ttaaacccta acaaaacaaa gagatggggt
     3061 tactctctaa attttatggg ttatgtcatt ggatgttatg ggtccttgcc acaagaacac
     3121 atcatacaaa aaatcaaaga atgttttaga aaacttccta ttaacaggcc tattgattgg
     3181 aaagtatgtc aacgaattgt gggtcttttg ggttttgctg ccccttttac acaatgtggt
     3241 tatcctgcgt tgatgccttt gtatgcatgt attcaatcta agcaggcttt cactttctcg
     3301 ccaacttaca aggcctttct gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa
     3361 cggccaggtc tgtgccaagt gtttgctgac gcaaccccca ctggctgggg cttggtcatg
     3421 ggccatcagc gcatgcgtgg aaccttttcg gctcctctgc cgatccatac tgcggaactc
     3481 ctagccgctt gttttgctcg cagcaggtct ggagcaaaca ttatcgggac tgataactct
     3541 gttgtcctat cccgcaaata tacatcgttt ccatggctgc taggctgtgc tgccaactgg
     3601 atcctgcgcg ggacgtcctt tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct
     3661 tctcggggtc gcttgggact ctctcgtccc cttctccgtc tgccgttccg accgaccacg
     3721 gggcgcacct ctctttacgc ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg
     3781 cacttcgctt cacctctgca cgtcgcatgg agaccaccgt gaacgcccac caaatattgc
     3841 ccaaggtctt acataagagg actcttggac tctcagcaat gtcaacgacc gaccttgagg
     3901 catacttcaa agactgtttg tttaaagact gggaggagtt gggggaggag attaggttaa
     3961 aggtctttgt actaggaggc tgtaggcata aattggtctg cgcaccagca ccatgcaact
     4021 ttttcacctc tgcctaatca tctcttgttc atgtcctact gttcaagcct ccaagctgtg
     4081 ccttgggtgg ctttggggca tggacatcga cccttataaa gaatttggag ctactgtgga
     4141 gttactctcg tttttgcctt ctgacttctt tccttcagta cgagatcccc gggcgagctc
     4201 ggtaccaagc ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc
     4261 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4321 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4381 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4441 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4501 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4561 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4621 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
     4681 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4741 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4801 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4861 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
     4921 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4981 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5041 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5101 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5161 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5221 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5281 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     5341 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     5401 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     5461 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     5521 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     5581 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     5641 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     5701 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     5761 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5821 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5881 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5941 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6001 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6061 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6661 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6901 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8221 gacgtc
//
