LOCUS       V015381                 8240 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V015381
VERSION     V015381
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1324..3852
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype C subtype
                     ad (isolate Japan/S-179/1988). Accession#: P03157"
     polyA_signal    4253..4477
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4523..4951
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4965..5295
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5343..5390
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5409..5780
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5913..6046
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6099)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6107..6123)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6131..6161)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6176..6197)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6485..7073)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7247..8104)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8105..8209)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcatgttcat gtcctactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttagggcatg gacattgacc cgtataaaga atttggagca tctacggagt
      961 tactctcttt tttgccttct gacttttttc cttctattcg agatctcctc gacaccgcct
     1021 cagctctgta tcgggaggcc ttagagtctc ctgaacattg ttcacctcac catacggcac
     1081 tcaggcaagc cattctgtgt tggggtgagt tgatgaatct ggccacctgg gtgggaggta
     1141 atttggaaga cccagcatcc agggaattag tagtcggcta tgtcaatgtt aatatgggcc
     1201 taaaactcag acaactactt tggtttcaca tctcctgtct tacttttgga agagaaactg
     1261 ttcttgaata tttggtgtct tttggagtgt ggattcgcac tcctcctgct tacagaccac
     1321 caaatgcccc tatcttatca acacttccgg aaactactgt tgttagacga cgaggcaggt
     1381 cccctagaag aagaactccc tcgcctcgca gacgaaggtc tcaatcgccg cgtcgcagaa
     1441 gatctcaatc tcgggaatct caatgttagt atcccttgga ctcataaggt gggaaacttt
     1501 actgggcttt attcttctac ggtacctgtc tttaatcctg agtggcaaac tccctccttt
     1561 cctcacattc atttgcagga ggacattatt gatagatgtc aacaatttgt gggccctctt
     1621 acagttaatg aaaaaaggag attaaaatta attatgcctg ctaggttcta tcctaacctt
     1681 accaaatatt tgcccttgga caaaggcatt aaaccatatt atcctgaaca tgcagttaat
     1741 cattacttca aaactaggca ttatttacat actctgtgga aggcgggaat tctatataag
     1801 agagaaacta cacgcagcgc ctcattttgt gggtcaccat attcttggga acaagagcta
     1861 cagcatggga ggttggtctt ccaaacctcg acaaggcatg gggacgaatc tttcggttcc
     1921 caatccgctg ggattctttc ccgatcacca gttggaccct gcgttcggag ccaactcaaa
     1981 caatccagat tgggacttca accccaacaa ggatcactgg ccagaggcaa atcaggtagg
     2041 agcgggagca ttcgggccag ggttcacccc accacacggc ggtcttgtgg ggtggagccc
     2101 tcaggctcag ggcatattga caacagtgcc agtagcacct cctcctgcct ccaccaatcg
     2161 gcagtcagga agacagccta ctcccatctc tccacctcta agagacagtc atcctcaggc
     2221 catgcagtgg aactccacca cattccacca agctctgcta gaccccagag tgaggggcct
     2281 ataccttcct gctggtggct ccagttccgg aacagtaaac cctgttccga ctactgcctc
     2341 acccatatcg tcaatcttct cgaggactgg ggaccctgca ccgagcatgg agaacacaac
     2401 atcaggattc ctaggacccc tgctcgtgtt acaggcgggg tttttcttgt tgacaagaat
     2461 cctcacaata ccacagagtc tagactcgtg gtggacttct ctcaattttc tagggggagc
     2521 acccacgtgt cctggccaaa attcgcagtc cccaacctcc aatcactcac caacctcttg
     2581 tcctccaatt tgtcctggct atcgctggat gtgtctgcgg cgttttatca tattcctctt
     2641 catcctgctg ctatgcctca tcttcttgtt ggttcttctg gactaccaag gtatgttgcc
     2701 cgtttgtcct ctacttccag gaacatcaac taccagcacg ggaccatgca agacctgcac
     2761 gattcctgct caaggaacct ctatgtttcc ctcttgttgc tgtacaaaac cttcggacgg
     2821 aaactgcact tgtattccca tcccatcatc ctgggctttc gcaagagtcc tatgggagtg
     2881 ggcctcagtc cgtttctcct ggctcagttt actagtgcca tttgttcagt ggttcgtagg
     2941 gctttccccc actgtttggc tttcagttat atggatgatg tggtattggg ggccaagtct
     3001 gtacaacatc ttgagtccct ttatacctct attaccaatt ttcttttgtc tttgggtata
     3061 catttaaacc ctaataaaac caaacgttgg ggctactccc ttaatttcat gggatatgta
     3121 attggatgtt ggggtacttt acctcaagaa catattgtac aaaaaatcaa gcactgtttt
     3181 cggaaactgc ctgtcaatag acctattgat tggaaagtat gtcagagaat tgtgggtctt
     3241 ttgggctttg ctgccccttt tacacaatgt ggctatcctg ccttaatgcc tttatatgca
     3301 tgtatacaat ctaagcaggc tttcactttc tcgccaactt acaaggcctt tctgtgtaaa
     3361 caatatctga acctttaccc cgttgcccgg caacggtcag gtctttgcca agtgtttgct
     3421 gacgcaaccc ccactggatg gggcttggct attggccatc gccgcatgag tggaaccttt
     3481 gtggctcctc tgccgatcca tactgcggaa ctcctagcag cttgttttgc tcgcagccgg
     3541 tctggagcga aacttatcgg aaccgacaac tctgttgtcc tctctcggaa atacacctcc
     3601 tttccatggc tgctagggtg tgctgccaac tggatcctgc gcgggacgtc ctttgtctac
     3661 gtcccgtcgg cgctgaatcc cgcggacgac ccgtctcggg gccgtttggg actttaccgt
     3721 ccccttcttc gtctgccgtt ccggccgacc acggggcgca cctctcttta cgcggtctcc
     3781 ccgtctgtgc cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca
     3841 tggagaccac cgtgaacgcc caccaggtct tgcccaaggt cttatataag aggactcttg
     3901 gactcgcagc aatgtcaacg accgaccttg aggcatactt caaagactgt ttgtttaagg
     3961 actgggagga gttgggggag gagattaggt taatgatctt tgtactagga ggctgtaggc
     4021 ataaattggt ctgttcacca gcaccatgca actttttcac ctctgcctaa tcatctcatg
     4081 ttcatgtcct actgttcaag cctccaagct gtgccttggg tggctttagg gcatggacat
     4141 tgacccgtat aaagaatttg gagcatctac ggagttactc tcttttttgc cttctgactt
     4201 ttttccttct attcgagatc aagcttaagt ttaaaccgct gatcagcctc gactgtgcct
     4261 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4321 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4381 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4441 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4501 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     4681 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4861 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     4921 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4981 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     5041 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5101 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5161 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5221 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5281 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5341 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5401 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5461 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     5521 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     5581 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     5641 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     5701 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     5761 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     5821 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5881 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6061 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8221 gaaaagtgcc acctgacgtc
//
