LOCUS       V015700                 8240 bp    DNA     circular SYN 21-DEC-2019
DEFINITION  Exported.
ACCESSION   V015700
VERSION     V015700
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg
            69120, Deutschland
REFERENCE   2  (bases 1 to 8240)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-NOV-2019) Molecular Virology, Department of Infectiology,
            Heidelberg University Hospital, Inf344, Heidelberg 69120,
            Deutschland"
FEATURES             Location/Qualifiers
     source          1..8240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1324..3852
                     /gene="P"
                     /label="Protein P"
                     /note="Protein P from Hepatitis B virus genotype B/C
                     subtype adw (isolate Okinawa/pODW282/1998). Accession#:
                     P17395"
     polyA_signal    4253..4477
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4523..4951
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4965..5295
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        5343..5390
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5409..5780
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5913..6046
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6099)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6107..6123)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6131..6161)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6176..6197)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6485..7073)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7247..8104)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8105..8209)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcatgttcat gtcctactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacattgacc cgtataaaga atttggagct tctgtggagt
      961 tactctcttt tttgccttcc gacttctttc cttctattcg agatcttctc gacaccgcct
     1021 ctgctctgta tcgggaggcc ttagagtctc cggaacattg ttcacctcac catacggcac
     1081 tcaggcaagc tattctgtgt tggggtgagt tgatgaatct agccacctgg gtgggaagta
     1141 atttggaaga tccagcatcc agggaattag tagtcagcta tgtcaatgtt aatatgggcc
     1201 taaaaatcag acaactattg tggtttcaca tttcctgtct tacttttgga agagaaactg
     1261 ttctcgaata tttggtgtct tttggagtgt ggattcgcac tcctcctgca tatagaccac
     1321 caaatgcccc tatcttatca acacttccgg aaactactgt tgttagacga cgaggcaggt
     1381 cccctagaag aagaactccc tcgcctcgca gacgaaggtc tcaatcaccg cgtcgcagaa
     1441 gatctcaatc tcgggaatct caatgttagt attccttgga ctcataaggt gggaaacttt
     1501 acggggcttt attcttctac tgtacctaac tttaatcctg atcggcaaac tccttctttt
     1561 cctgacattc atttgcagga ggacattgct gataggtgta agcaatttgt gggaccccta
     1621 acagtaaatg aaaacaggag attaaaatta attatgcctg ctagatttta tcccaatgtt
     1681 accaaatact tgcccttaga taaaggaatt aaaccctatt atccagagca tgtagttaat
     1741 cattacttcc agacgagaca ttatttacat actctttgga aggcgggtat cttatataaa
     1801 agagagacga cacgtagcgc ctcattttgc gggtcaccat attcttggga acaagagcta
     1861 cagcatggga ggttggtctt ccaaacctcg aaaaggcatg gggacaaatc tttctgtccc
     1921 caatccactg ggattcttcc ccgatcatca gttggaccct gcattcaaag ccaactcaga
     1981 aaatccagat tgggacctca atccatacaa ggacaactgg ccggatgcca acaaggtggg
     2041 agtgggagca ttcgggccag ggttcacccc tccccatggg ggcctgttgg ggtggagccc
     2101 tcaggctcag ggcatactca caactgtgcc agcagcacct cctcctgcct ccaccaatcg
     2161 gcagtcagga aggcagccta ctcccttatc tccacctcta agggacactc atcctcaggc
     2221 catgcagtgg aactccacca ctttccatca aactcttcaa gatcccagag tcagggctct
     2281 gtactttcct gctggtggct ccagttcagg aacagtaagc cctgctcaga atactgcctc
     2341 tgccatatcg tcaaccttct cgaagactgg ggaccctgta ccgaacatgg agaacatcgc
     2401 atcaggactc ctaggacccc tgctcgtgtt acaggcgggg tttttcttgt tgacaaaaat
     2461 cctcacaata ccacagagtc tagactcgtg gtggacttct ctcaattttc tagggggaac
     2521 acccgtgtgt cttggccaaa attcgcagtc ccaaatctcc agtcactcac caacttgttg
     2581 tcctccaatt tgtcctggtt atcgctggat gtgtctgcgg cgttttatca tcttcctctg
     2641 catcctgctg ctatgcctca tcttcttgtt ggttcttctg gactatcaag gtatgttgcc
     2701 cgtttgtcct ctaattccag gatcatcaac caccagcacc ggaccatgca gaacctgcac
     2761 gactcctgct caaggaacct ctatgtttcc ctcatgttgc tgtacaaaac ctatggacgg
     2821 aaactgcaca tgtattccca tcccatcatc ttgggctttc gcaaaatacc tatgggagtg
     2881 ggcctcagtc cgtttctctt ggctcagttt actagtgcca tttgttcagt ggttcgtagg
     2941 gctttccccc actgtctggc tttcagttat atggatgata tggtattggg ggccaagtct
     3001 gtacaacatc ttgagtccct ttatgccgct gttaccaatt ttcttttgtc tttgggtata
     3061 catttaaacc ctcataaaac aaaaagatgg ggatattccc ttaatttcat gggatatgta
     3121 attgggagtt ggggctcctt gccacaggaa catattgtac aaaaaatcaa attatgtttt
     3181 agaaaacttc ctgtcaacag gcctattgat tggaaagtgt gtcaacgaat tgtaggtctt
     3241 ttggggttcg ctgccccttt tacacaatgt ggatttcctg ctttaatgcc tttatatgca
     3301 tgtattcaag ctaaacaggc ttttactttc tcgccaactt acaaggcctt tctaagcaaa
     3361 cagtatctga acctttaccc cgttgctcgg caacggcctg gtctgtgcca agtgtttgct
     3421 gacgcaaccc ccactggttg gggcttggcc ataggccatc agcgcatgcg cggaaccttt
     3481 gtgtctccgc tgccgatcca tactgcggaa ctcctagcag cttgttttgc tcgcagccgg
     3541 tctggagcga aacttatcgg gactgataat tctgtcgtgc tctcccgcaa gtatacgtcg
     3601 tttccatggc tgctaggctg tgctgccaac tggatcctgc gcgggacgtc ctttgtttac
     3661 gtcccgtcgg cgctgaatcc cgcggacgac ccctcccggg gccgcttggg gctctaccgg
     3721 ccgcttctcc gtctcccgta ccgaccgacc acggggcgca cctctcttta cgcggactcc
     3781 ccgtctgtgc cttctcatct gccggaccgt gtgcacttcg cttcacctct gcacgtcgca
     3841 tggagaccac cgtgaacgcc cactggaacc tgcccaaggt cttacaggag aggactcttg
     3901 gactttcagc aatgtcaacg accgaccttg aggcatactt caaagactgt gtgtttgctg
     3961 agtgggagga gttgggggag gagattaggt taaaggtctt tgtattagga ggctgtaggc
     4021 ataaattggt ctgttcacca gcaccatgca actttttcac ctctgcctaa tcatctcatg
     4081 ttcatgtcct actgttcaag cctccaagct gtgccttggg tggctttggg gcatggacat
     4141 tgacccgtat aaagaatttg gagcttctgt ggagttactc tcttttttgc cttccgactt
     4201 ctttccttct attcgagatc aagcttaagt ttaaaccgct gatcagcctc gactgtgcct
     4261 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4321 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4381 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4441 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     4501 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc
     4681 atccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4861 tcggtctatt cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat
     4921 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     4981 gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     5041 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5101 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5161 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5221 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5281 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     5341 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     5401 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     5461 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     5521 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     5581 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     5641 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     5701 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     5761 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     5821 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5881 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5941 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     6001 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     6061 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8221 gaaaagtgcc acctgacgtc
//
