LOCUS       62056_6095        8246 bp DNA     circular SYN 21-DEC-2019
DEFINITION  Cloning vector pcDNA3.1Zeo(-)-HBV1.1- A2, complete sequence.
ACCESSION   MN645903
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Wang W, Ni Y, Urban S.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2019) Molecular Virology, Department of 
            Infectiology, Heidelberg University Hospital, Inf344, Heidelberg 
            69120, Deutschland
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2019) Molecular Virology, Department of Infectiology, 
            Heidelberg University Hospital, Inf344, Heidelberg 69120, 
            Deutschland"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             918..1475
                     /codon_start=1
                     /transl_table=11
                     /product="core protein"
                     /label=core protein
                     /protein_id="QGX02055.1"
                     /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP
                     EHCSPHHTALRQAILCWGELMTLATWVGNNLEDPASRDLVVNYVNTNMGLKISQLLWFH
                     ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRDRGRSPRRRTP
                     SPRRRRSQSPRRRRSQSRESQC"
     CDS             2393..3070
                     /label=HBsAg (S)
                     /note="major/small (S) envelope protein of hepatitis B
                     virus"
     CDS             3612..4076
                     /codon_start=1
                     /transl_table=11
                     /product="X protein"
                     /label=X protein
                     /protein_id="QGX02057.1"
                     /translation="MAARLCCQLDPSRDVLCLRPVGAESRGRPLSGPLGTLSSPSPSAV
                     PADHGAHLSLRGLPVCAFSSAGPCALRFTSARCMETTVNAHQILPKVLHKRTLGLPAMS
                     TTDLEAYFKDCVFKDWEELGEEIRLKVFVLGGCRHKLVCAPAPCNFFTSA"
     polyA_signal    4259..4483
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4529..4957
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4971..5301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        5349..5396
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             5415..5786
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    5919..6052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6089..6105)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6113..6129)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6137..6167)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6182..6203)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6491..7079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7253..8110)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8111..8215)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tcgacgcacc atgcaacttt
      841 ttcacctctg cctaatcatc tcttgtacat gtcccactgt tcaagcctcc aagctgtgcc
      901 ttgggtggct ttggggcatg gacattgacc cttataaaga atttggagct actgtggagt
      961 tactctcgtt tttgccttct gacttctttc cttccgtcag agatctccta gacaccgcct
     1021 cggctctgta tcgggaagcc ttagagtctc ctgagcattg ctcacctcac cataccgcac
     1081 tcaggcaagc cattctctgc tggggggaat tgatgactct agctacctgg gtgggtaata
     1141 atttggaaga tccagcatcc agggatctag tagtcaatta tgttaatact aacatgggat
     1201 taaagatcag ccaactcttg tggtttcata tctcttgcct tacttttgga agagaaactg
     1261 tacttgaata tttggtctct ttcggagtgt ggattcgcac tcctccagcc tatagaccac
     1321 caaatgcccc tatcttatca acacttccgg aaactactgt tgttagacga cgggaccgag
     1381 gcaggtcccc tagaagaaga actccctcgc ctcgcagacg cagatctcaa tcgccgcgtc
     1441 gcagaagatc tcaatctcgg gaatctcaat gttagtattc cttggactca taaggtggga
     1501 aacttcactg ggctttattc ctctacagca cctatcttta atcctgaatg gcaaactcct
     1561 tcctttccta aaattcattt acaagaggac attattaata ggtgtcaaca atttgtgggc
     1621 cctctcactg taaatgaaaa gagaagattg aaattaatta tgcctgctag attctatcct
     1681 acccacacta aatatttgcc cttagacaaa ggaattaaac cttattatcc agatcaggta
     1741 gttaatcatt acttccaaac cagacattat ttacatactc tttggaaggc gggtattcta
     1801 tataagagag aaaccacacg tagcgcatca ttttgcgggt caccatattc ttgggaacaa
     1861 gagctacagc atgggaggtt ggtcatcaaa acctcgcaaa ggcatgggga cgaatctttc
     1921 tgttcccaac cctctgggat tctttcccga tcatcagttg gaccctgtat tcggagccaa
     1981 ctcaaacaat ccagattggg acttcaaccc catcaaggac cactggccag cagccaacca
     2041 ggtaggagtg ggagcattcg ggccagggtt cacccctcca cacggcggtg ttttggggtg
     2101 gagccctcag gctcagggca tgttgacccc agtgtcaaca attcctcctc ctgcctccgc
     2161 caatcggcag tcaggaaggc agcctactcc catctctcca cctctaagag acagtcatcc
     2221 tcaggccatg cagtggaatt ccactgcctt ccaccaagct ctgcaagacc ccagagtcag
     2281 gggtctgtat tttcctgctg gtggctccag ttcaggaaca gtaaaccctg ctccgaatat
     2341 tgcctctcac atctcgtcaa tctccgcgag gactggggac cctgtgacga acatggagaa
     2401 catcacatca ggattcctag gacccctgct cgtgttacag gcggggtttt tcttgttgac
     2461 aagaatcctc acaataccgc agagtctaga ctcgtggtgg acttctctca attttctagg
     2521 gggatcaccc gtgtgtcttg gccaaaattc gcagtcccca acctccaatc actcaccaac
     2581 ctcatgtcct ccaatttgtc ctggttatcg ctggatgtgt ctgcggcgtt ttatcatatt
     2641 cctcttcatc ctgctgctat gcctcatctt cttattggtt cttctggatt atcaaggtat
     2701 gttgcccgtt tgtcctctaa ttccaggatc aacaacaacc agtacgggac catgcaaaac
     2761 ctgcacgact cctgctcaag gcaactctat gtttccctca tgttgctgta caaaacctac
     2821 ggatggaaat tgcacctgta ttcccatccc atcgtcttgg gctttcgcaa aatacctatg
     2881 ggagtgggcc tcagtccgtt tctcttggct cagtttacta gtgccatttg ttcagtggtt
     2941 cgtagggctt tcccccactg tttggctttc agctatatgg atgatgtggt attgggggcc
     3001 aagtctgtac agcatcgtga gtccctttat accgctgtta ccaattttct tttgtctctg
     3061 ggtatacatt taaaccctaa caaaacaaaa agatggggtt attccctaaa cttcatgggt
     3121 tatgtaattg gaagttgggg gacattgcca caggatcata ttgtacaaaa aatcaaacac
     3181 tgttttagaa aacttcctgt taacaggcct attgattgga aagtatgtca aagaattgtg
     3241 ggtcttttgg gctttgctgc tccttttaca caatgtggat atcctgcctt aatgcccttg
     3301 tatgcatgta tacaagctaa acaggctttc actttctcgc caacttacaa ggcctttcta
     3361 agtaaacagt acatgaacct ttaccccgtt gctcggcaac ggcctggtct gtgccaagta
     3421 tttgctgatg caacccccac tggctggggc ttggccatag gccatcagcg catgcgcgga
     3481 acctttgtgg ctcctctgcc gatccatact gcggaactcc tagccgcttg ttttgctcgc
     3541 agccggtctg gagcgaaact catcggaact gacaattctg tcgtcctctc gcggaaatat
     3601 acctcgtttc catggctgct aggctgtgct gccaactgga tccttcgcgg gacgtccttt
     3661 gtttacgtcc cgtcggcgct gaatcccgcg gacgacccct ctcggggccg cttgggactc
     3721 tctcgtcccc ttctccgtct gccgttccag ccgaccacgg ggcgcacctc tctttacgcg
     3781 gtctccccgt ctgtgccttc tcatctgccg gtccgtgtgc acttcgcttc acctctgcac
     3841 gttgcatgga gaccaccgtg aacgcccatc agatcctgcc caaggtctta cataagagga
     3901 ctcttggact cccagcaatg tcaacgaccg accttgaggc ctacttcaaa gactgtgtgt
     3961 ttaaggactg ggaggagctg ggggaggaga ttaggttaaa ggtctttgta ttaggaggct
     4021 gtaggcataa attggtctgc gcaccagcac catgcaactt tttcacctct gcctaatcat
     4081 ctcttgtaca tgtcccactg ttcaagcctc caagctgtgc cttgggcggc tttggggcat
     4141 ggacattgac ccttataaag aatttggagc tactgtggag ttactctcgt ttttgccttc
     4201 tgacttcttt ccttccgtca gagatcaagc ttaagtttaa accgctgatc agcctcgact
     4261 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4321 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4381 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4441 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4501 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4561 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4621 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4681 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4741 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4801 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4861 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
     4921 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     4981 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5341 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     5401 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     5461 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     5521 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     5581 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     5641 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     5701 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     5761 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     5821 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5881 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6061 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6121 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6181 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6241 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6301 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6661 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     6721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6901 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     6961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7141 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7201 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7261 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7321 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7381 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7441 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7501 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7561 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7621 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7681 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7741 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7801 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7861 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7921 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7981 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8041 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8101 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8161 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8221 ttccccgaaa agtgccacct gacgtc
//
