LOCUS       V046506                11016 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046506
VERSION     V046506
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..5838
                     /label="Zfp831(NM_001099328)"
                     /note="Zfp831(NM_001099328)"
                     /gene="Zfp831"
     CDS             5875..6585
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    6611..6835
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6881..7309
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7323..7652
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7719..8513
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8689..8810
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8859..8875)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8883..8899
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8907..8937)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8952..8973
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9261..9849)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10020..10880)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10881..10985)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggag
      961 gcgtcagaac cgacccaccc taccccatcg gccacggacc agccagcctc tgtacccggc
     1021 attccagggg gcctaggggg gcaggcctca cctcgcctga ccctgggtcc tgttatcctg
     1081 ccaccagagc agggcctggc cccaactgtg ttcttgaagg cattgcctgt ccccttatac
     1141 cacacggtac ctcctggggg cctccagcca cgtgcacccc tggtgacagg cagcctggag
     1201 ggggccagcg tacctttcat tctcagcccc ttgctgcagc ctgaaggacc cagtgccacc
     1261 caggtgggca agccagccac ccccgccctg actgtgaaca ttgtaggcac attgcctgta
     1321 ctctcctcgg gtgtggggtc tgcactggtc agcacaggca gagtgaagaa tactggtaag
     1381 tacctgtgcc cccactgtgg ccgggactgc ttgaagccca gtgtgctgga gaaacacatc
     1441 cggtcccaca caggtgaaag gcccttcccg tgccctacct gtggcatcgc ctttaagacc
     1501 cagagcaacc tgtataaaca caggcggaca caaacacatc tcaacaactc ccgcttgtcc
     1561 ttggagtctg aggggagtgg gagcagcctt ctggaggagg gagacaggcc cggagagacc
     1621 gcctcagtgg atagtcgtgg agatgaaggt gctccagaga gagccctttc cccaggtgcc
     1681 ctccccgccc ttgctgctca gaacagggaa aggaaggtgg aatcggctcc ctctctcggg
     1741 gccacccttt cccacaggga ggcctctgcg gatcacactc agatggtgtc acctgagttt
     1801 tcactagcca gcccgcagcc taggcggaag ctgccagaac caaagccatg cttcctgcag
     1861 cgacagcagg agacctgttc agagaagccc tgggacccca aggccttgga gggccagctg
     1921 aggaagtgtg agagcacaga ctctggctac ctgtcgcgct cagacagcgt ggagcagccc
     1981 cctgttgcct ctagcccatt gcatagtcta tcggagcaca gtgcggagtc tgagggggaa
     2041 ggaggccctg gctgttcctc ttccggcaac agggctgaac gaggaacccc agggcccagc
     2101 ctggagttag agaagaagaa actggaggaa cgaattgccc agctcatctc ccacaaccag
     2161 gcggtggtag atgacccaca gttggaccat gtgcggcccc gcaagactgt gctgtccaag
     2221 cagggcagca tcgacctgcc catgccatat acctacaagg attccttcca ctttgacatc
     2281 cgggctctgg agcccagccg caggagggct gccctcagcc cggcccgctc caccttcaca
     2341 cccctggaaa aggccagacc tctctttttc cactcagtcc ccacgcagct ctccactaca
     2401 gtggagtgtg tccctgtcac cagaagcaac tcgctgcctt tcgtcgaggg ttctaagttg
     2461 tggccagagc aacgggaccc acaggatgct gtcccaggaa tgcagaaggc cctgagcccc
     2521 aggcccaccc cagcccggct gggatgccgc ccaggactga ccttggcaag catctccagt
     2581 ggtcacccac gggccctggt aagacaggct gctgtggagg acctgccgtg taccccgact
     2641 ggagaccctt cagccgctgc tgaggaacta gatggaaaga gagctgtggc aaaggagggt
     2701 gtggtgtaca agggcagagc cagtaaaaag tgtagccaga ggaagcaaag gatgttctcc
     2761 caggagaaat ggcaagtgta tggaaatgag actttcaaga gaatatacca gaaaatgaag
     2821 accaaccacc agggaggaca gaaacacagg caggtgaaac agggccaggg gacagaactg
     2881 gactttcctc ctcctcctcc tcctcagaaa caggggggag gaggtgagga tacaggcctg
     2941 tctcaagaca gtaggatccc tatccttgga gacactacag caggatgtaa gctagagtct
     3001 tggggaagta gaccatccct ggaagactca ctggaggctg agtctcctaa gcaactgaag
     3061 gtggtgacca gagtcaaatc atctcctacc ctcggcagca cagatagcct ctgtttgagt
     3121 ggcaagagct ctttgctctc tccaagtggg aaattagatc tgggatgcca gccgcctccg
     3181 gtgcctacgg cttgccaagc aggagattta gaagctcccg agactgcttc accagactca
     3241 agtggtgagg ctataaaaga aaccagccct ggagcccaaa ccgtcctcag acggtccagt
     3301 gatgggtctg tggctctaca gcttgcagaa gacaagttac cttcagagag gaagagactg
     3361 aaggtggaag ggctggacca tcaggagcag ccagggcctc tggaagctga tacctcagga
     3421 ggctctgtgc aggctgaccc cccaccgtcc gagagacagg acagtgactc tgaggagttg
     3481 ctgggttgct caaataagag taccctgcat gtgaccacag ccccggagcc ctcagatgtc
     3541 tcctcagccg cttctcctgt tgccctgcaa tggccaggga taggggacaa ggattcccca
     3601 ttgccttctg tacccaggac tcctgggctt cactccctct tgcctgctca gccccaggat
     3661 cctggtgtcc ctaatgctcg gtcaggtggc ttctttcctc ccaagtacct cctcaggtta
     3721 cctcagggag agaacccctc accgatagtt gctgcagggc ctgaaaaagg ctcagactct
     3781 ctgtgtacaa gtacacagcc tgtagaacag gcccttctgg ttggatcaga actcggaatg
     3841 ctccggccac ctggtgcata ctcaggccca ggtgaggcag gtggcagccc agacgaccct
     3901 agctggtcca ggttaaggga tggaaaagaa gaggcacaga caggaagaga gaaaagagat
     3961 aggaccaagg acaacatcct agtggttggg cactctcctg ttccaattac cagcaccacc
     4021 caggagatag cctccgttct gtctactcac acctgtgaca ttcgggtggc ccaggatgta
     4081 gtgagtaaga cccatgccat ccattgcctc tgcacagaca gcccaccagc aagagctagg
     4141 acatatggga atctcttaaa cccttggaca tcatcccaga agctggggat accgcccaag
     4201 agtgccctag aaggtcctcc ttcagaaacc aaggaagggc tgcctgcttt cttttcctct
     4261 caagcacact atttccttac aacttttaca aaaccccagg atatttcctt gaaccagcag
     4321 aaaccggcct tgtcacacgt cttaggaacc cccaggagct gtgaggcccc aagctccttc
     4381 ccctcactta agactgaacc ccagctcaca tggtgttgct taagtcgaag cctgcccctg
     4441 cctgcagagc agcggaggat agctcctgga cacttggctt catccttgcc taatagaaac
     4501 ctccagggcg acagtaacaa aacactaccc aagagcaatg gaggaaggac ccagacaagc
     4561 caaggaggag aagtgtctac acaggcgcct gagcttccct atccatcagt cccagagctg
     4621 agggctgagg accaagtatc caggccacgg tggaagaaag aacaactatg gcgtagaaag
     4681 gtgaagacat ctcgtgaaaa caccaaacag aagaaactga ggcttcattc taaaaggtgt
     4741 gaagggagtt tctggcagag agccaaacgc ctgtgcaagc caccctggtt gccaagaaga
     4801 agctgtctcc cccaccgact tgaggggtta gagccacggg ggacccttgg gaggagttcg
     4861 gcagaagtgg caggtcgaaa tgtacaaggg gagccatctt gtgccagcaa agacttgtct
     4921 gtgtctcatg ggaacaaaga aaaggaaggt gactgtagtc agacttcgag acccatgtct
     4981 ccgggcatga gctcaaaaac catgttggaa atggacaaat cagctctgaa ggatatttct
     5041 ccaaccactg gtgagcataa tgactgttct caggagaagg agcatgctgg tgggtccgga
     5101 ctatctccac aatccaacac gttcttggca gtgtttcagg aagatgtgcc tcagggcaaa
     5161 ggtcttgatg ttggacctcc agaaactcag ctgccgcctt cccaggatca agtctctatc
     5221 actcccaact tgtgcttctc cccagatgtc ccagagctgc cttcttcctt taaacccaaa
     5281 ggaacatttc cccaccatga cgttgctacc tctgtggctg ccatctgtgt ttctgcgggg
     5341 ataagaacag gacgcaaaac cctgcgggct cacagtgcag aatcctgtgc agaggagact
     5401 ctggcacgaa actccccaga tagaagaggc acaccggaca gcattccacg ggtgctcctg
     5461 ccagggaggc cttcacctgg taaacaatca ccaggacaaa ctctagaaat accaagttca
     5521 ggaccaagtt cagtcagttc actccaggag gaagcaagag cccaggcaga ttttccttgg
     5581 tggggccagt acggatgtgg ggacctgacc gtccagggtg ctgcttcagg aagtgagagt
     5641 aggacttgtc aagctgaagg attaattacc tccaagactg tcacagctcc ttctgaccca
     5701 ggacaaccct cacaagtccc cgaggaggcc cctttgagat ccatccgaaa gaggagtctg
     5761 gaaggaatga ggaagcagac acgggtggag ctcagcgata ccagcagtga cgatgaagac
     5821 agactggtca ttgagatcct cgagtctaga gggcccttcg ggggtggagg ctctatggtg
     5881 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     5941 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     6001 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     6061 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     6121 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     6181 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     6241 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     6301 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     6361 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     6421 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     6481 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     6541 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagttta aacccgctga
     6601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6781 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6841 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7081 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     7201 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     7261 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     7321 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7381 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7441 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7501 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7561 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7621 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7681 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     7741 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7801 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7861 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     7921 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7981 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     8041 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     8101 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     8161 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     8221 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     8281 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     8341 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     8401 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     8461 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     8521 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     8581 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8641 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8701 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8761 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8821 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8881 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8941 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9001 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9061 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9121 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9181 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9241 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9301 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9361 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9421 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9481 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9541 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9601 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9661 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9721 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9781 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9841 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9901 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9961 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10021 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10081 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10141 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10201 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10261 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10321 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10381 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10441 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10501 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10561 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10621 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10681 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10741 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10801 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10861 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10921 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10981 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
