LOCUS       V039204                 7972 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039204
VERSION     V039204
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             964..3462
                     /label="ZNF780B(NM_001005851)"
                     /note="ZNF780B(NM_001005851)"
                     /gene="ZNF780B"
     CDS             3469..3534
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3572..3796
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3842..4270
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4284..4613
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4680..5474
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5648..5769
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5818..5834)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5842..5858
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5866..5896)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5911..5932
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6220..6805)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6976..7836)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7837..7941)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc
      961 accatggtcc atggatcagt gacattcagg gatgtggcca ttgacttctc tcaggaggag
     1021 tgggagtgcc tgcagcctga tcagaggacc ttgtacaggg atgtgatgtt ggagaactac
     1081 agccacctga tatcactggg aagttccatt tctaagccag atgtgattac attactagag
     1141 caagagaaag agccctggat tgttgtaagt aaagaaacaa gcagatggta tccagatttg
     1201 gagtcaaaat atggacctga gaaaatatct ccagaaaatg atatttttga aataaattta
     1261 cccaaacatg ttataaagca aataagtaaa acacttggcc tcgaggcctt ttattttaga
     1321 aatgactcag aatatagaag tagatttgag ggacgacagg gacatcaaga aggatatatc
     1381 aaccagaaga tcatcagcta tgaagaaatg cctgcttata ctcatgcttc tcctattcat
     1441 aatacacata aaccatatga atgtaaggaa tgtgggaaat actttagttg tggttcaaat
     1501 cttattcagc atcagagtat tcatactgga gagaaaccct ataaatgcaa agaatgtggg
     1561 aaagcctttc aacttcacat acaacttact cgacatcaga aatttcatac tggtgagaaa
     1621 acttttgaat gtaaggaatg tggaaaagcc tttaatcttc ccacccagct taatcgccat
     1681 aagaacattc acacagttaa gaaactgttt gaatgtaagg aatgtgggaa gtcttttaat
     1741 cgtagctcaa accttactca gcatcaaagt attcatgctg gtgtaaaacc atatcaatgt
     1801 aaggagtgtg ggaaagcctt taatcgtggt tcaaatctta ttcagcatca aaaaattcat
     1861 tccaatgaga aaccctttgt atgtagggaa tgtgagatgg cctttcgata tcattaccaa
     1921 ctcattgaac attgccgaat tcatactggc gagaaaccct ttgaatgtaa agaatgcaga
     1981 aaggccttta ctcttctgac aaagcttgtt cgacatcaga agattcatat gggtgagaag
     2041 ccctttgaat gcagggaatg cgggaaggcc tttagtctcc tcaatcagct taatcgccat
     2101 aagaatattc acacaggtga aaaaccattt gaatgtaaag aatgtgggaa gtcctttaat
     2161 cgtagttcaa accttattca acaccagagt attcatgctg atgtaaaacc atatgaatgt
     2221 aaggagtgtg ggaaaggctt taatcgtggt gcaaatctta ttcagcatca aaaaattcat
     2281 tccaatgaga aaccctttgt atgtagggaa tgtgagatgg cctttcgata tcattaccaa
     2341 cttattcaac attgccaaat tcatactggt gggaaaccct ttgaatgtaa agaatgtgga
     2401 aaggccttta gtcttctgac acagcttgct cgacataaga acattcatac tggtgagaaa
     2461 ccatttgaat gtaaagactg tgggaaggcc ttcaatcgtg gctcgaacct tgttcaacat
     2521 cagagtattc acactggtga gaagccctat gaatgtaagg agtgtgggaa ggcttttaga
     2581 cttcacctac aactttctca acatgagaaa actcatacag gtgagaaacc ctttgaatgt
     2641 aaggaatgtg ggaaattctt tcgtcgtggt tcaaatctta atcaacatcg aagtattcat
     2701 accggaaaga aaccctttga atgtaaggaa tgtgggaaag cctttcgact tcatatgcac
     2761 cttattcgac atcagaaatt tcatactggt gagaagccct ttgaatgtaa ggaatgtggc
     2821 aaggccttca gtcttcacac ccagcttaat caccataaga acattcacac aggtgagaag
     2881 ccatttaaat gtaaagaatg tgggaagtcc tttaatcgtg tctcaaacct tgttcaacat
     2941 cagagtattc atgctggtgt aaaaccatat gaatgtaagg agtgtgggaa aggctttagt
     3001 cgtgtttcaa accttattca gcatcagaaa actcattcca gtgcgaaacc ctttgtatgt
     3061 aaggagtgta ggaagacctt tagatatcat taccagctta ctgaacatta ccgaattcat
     3121 actggtgaga aaccctttga atgtaaagaa tgcggaaagg cctttggtct tctgacacag
     3181 cttgctcaac atcagatcat tcatactggt gagaagccat ttaaatgtaa ggagtgtggg
     3241 aaggccttta atcgtggctc aaaccttgtt caacctcaga gtattcatac tggtgagaaa
     3301 ccctatgaat gtaaggagtg tgggaaggct tttagacttc acctacaact ttctctgcat
     3361 caaaaacttg tacaggtgag aaaccctttg aatgtaagaa atgtgggaca gccttcagac
     3421 atcagtagca acttactgaa catcagaaaa ttcatacttg ggctcgagga ctacaaagac
     3481 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagtga
     3541 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3601 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3661 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3721 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3781 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3841 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3901 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3961 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4021 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4081 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4141 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4201 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4261 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4321 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4381 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4441 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4501 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4561 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4621 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4681 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4741 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4801 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4861 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4921 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4981 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5041 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5101 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5161 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5221 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5281 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5341 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5401 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5461 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5521 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5581 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5641 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5701 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5761 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5821 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5881 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5941 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6001 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6061 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6121 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6181 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6241 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6301 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6361 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6421 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6481 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6541 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6601 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6661 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6721 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
     6781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6841 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6901 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7261 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7561 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
