LOCUS V024877 7925 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024877 VERSION V024877 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 905..2731 /label="XRCC6(NM_001288976)" /note="XRCC6(NM_001288976)" /gene="XRCC6" CDS 2768..3487 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3525..3749 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3795..4223 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4237..4566 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4633..5427 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5601..5722 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5771..5787) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5795..5811 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5819..5849) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5864..5885 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6173..6758) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6929..7789) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7790..7894) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 caccatgtca gggtgggagt catattacaa aaccgagggc gatgaagaag cagaggaaga 961 acaagaagag aaccttgaag caagtggaga ctataaatat tcaggaagag atagtttgat 1021 ttttttggtt gatgcctcca aggctatgtt tgaatctcag agtgaagatg agttgacacc 1081 ttttgacatg agcatccagt gtatccaaag tgtgtacatc agtaagatca taagcagtga 1141 tcgagatctc ttggctgtgg tgttctatgg taccgagaaa gacaaaaatt cagtgaattt 1201 taaaaatatt tacgtcttac aggagctgga taatccaggt gcaaaacgaa ttctagagct 1261 tgaccagttt aaggggcagc agggacaaaa acgtttccaa gacatgatgg gccacggatc 1321 tgactactca ctcagtgaag tgctgtgggt ctgtgccaac ctctttagtg atgtccaatt 1381 caagatgagt cataagagga tcatgctgtt caccaatgaa gacaaccccc atggcaatga 1441 cagtgccaaa gccagccggg ccaggaccaa agccggtgat ctccgagata caggcatctt 1501 ccttgacttg atgcacctga agaaacctgg gggctttgac atatccttgt tctacagaga 1561 tatcatcagc atagcagagg atgaggacct cagggttcac tttgaggaat ccagcaagct 1621 agaagacctg ttgcggaagg ttcgcgccaa ggagaccagg aagcgagcac tcagcaggtt 1681 aaagctgaag ctcaacaaag atatagtgat ctctgtgggc atttataatc tggtccagaa 1741 ggctctcaag cctcctccaa taaagctcta tcgggaaaca aatgaaccag tgaaaaccaa 1801 gacccggacc tttaatacaa gtacaggcgg tttgcttctg cctagcgata ccaagaggtc 1861 tcagatctat gggagtcgtc agattatact ggagaaagag gaaacagaag agctaaaacg 1921 gtttgatgat ccaggtttga tgctcatggg tttcaagccg ttggtactgc tgaagaaaca 1981 ccattacctg aggccctccc tgttcgtgta cccagaggag tcgctggtga ttgggagctc 2041 aaccctgttc agtgctctgc tcatcaagtg tctggagaag gaggttgcag cattgtgcag 2101 atacacaccc cgcaggaaca tccctcctta ttttgtggct ttggtgccac aggaagaaga 2161 gttggatgac cagaaaattc aggtgactcc tccaggcttc cagctggtct ttttaccctt 2221 tgctgatgat aaaaggaaga tgccctttac tgaaaaaatc atggcaactc cagagcaggt 2281 gggcaagatg aaggctatcg ttgagaagct tcgcttcaca tacagaagtg acagctttga 2341 gaaccccgtg ctgcagcagc acttcaggaa cctggaggcc ttggccttgg atttgatgga 2401 gccggaacaa gcagtggacc tgacattgcc caaggttgaa gcaatgaata aaagactggg 2461 ctccttggtg gatgagttta aggagcttgt ttacccacca gattacaatc ctgaagggaa 2521 agttaccaag agaaaacacg ataatgaagg ttctggaagc aaaaggccca aggtggagta 2581 ttcagaagag gagctgaaga cccacatcag caagggtacg ctgggcaagt tcactgtgcc 2641 catgctgaaa gaggcctgcc gggcttacgg gctgaagagt gggctgaaga agcaggagct 2701 gctggaagcc ctcaccaagc acttccagga cggatccggc ggtggaggaa gcggcggagg 2761 tggatctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 2821 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 2881 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 2941 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3001 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3061 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3121 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3181 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3241 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3301 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3361 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3421 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3481 caagtagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg 3541 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 3601 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 3661 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 3721 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 3781 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 3841 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 3901 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 3961 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 4021 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 4081 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 4141 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 4201 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 4261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 4321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 4441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 4501 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 4561 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 4621 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 4681 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 4741 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 4801 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 4861 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 4921 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 4981 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 5041 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 5101 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 5161 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 5221 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 5281 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 5341 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 5401 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 5461 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 5521 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 5581 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 5641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 5701 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 5761 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 5821 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 5881 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 5941 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6001 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 6061 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 6121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 6181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 6241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 6301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 6361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 6421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 6481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 6541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 6601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 6661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 6721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 6781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 6841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 6901 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 6961 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 7021 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 7081 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 7141 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 7201 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 7261 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 7321 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 7381 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 7441 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 7501 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 7561 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 7621 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 7681 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 7741 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 7801 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 7861 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 7921 acgtc //