LOCUS       V033857                 7997 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033857
VERSION     V033857
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             905..2803
                     /label="XRCC1(NM_006297)"
                     /note="XRCC1(NM_006297)"
                     /gene="XRCC1"
     CDS             2840..3559
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3597..3821
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3867..4295
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4309..4638
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4705..5499
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5673..5794
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5843..5859)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5867..5883
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5891..5921)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5936..5957
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6245..6830)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7001..7861)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7862..7966)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccg gagatccgcc tccgccatgt cgtgtcctgc agcagccagg actcgactca
      961 ctgtgcagaa aatcttctca aggcagacac ttaccgaaaa tggcgggcag ccaaggcagg
     1021 cgagaagacc atctctgtgg tcctacagtt ggagaaggag gagcagatac acagtgtgga
     1081 cattgggaat gatggctcag ctttcgtgga ggtgctggtg ggcagttcag ctggaggcgc
     1141 tggggagcaa gactatgagg tccttctggt cacctcatct ttcatgtccc cttccgagag
     1201 ccgcagtggc tcaaacccca accgcgttcg catgtttggg cctgacaagc tggtccgggc
     1261 agccgccgag aagcgctggg accgggtcaa aattgtttgc agccagccct acagcaagga
     1321 ctcccccttt ggcttgagtt ttgtacggtt tcatagcccc ccagacaaag atgaggcaga
     1381 ggccccgtcc cagaaggtga cagtgaccaa gcttggccag ttccgtgtga aggaggagga
     1441 tgagagcgcc aactctctga ggccgggggc tctcttcttc agccggatca acaagacatc
     1501 cccagtcaca gccagcgacc cagcaggacc tagctatgca gctgctaccc tccaggcttc
     1561 tagtgctgcc tcctcagcct ctccagtctc cagggccata ggcagcacct ccaagcccca
     1621 ggagtctccc aaagggaaga ggaagttgga tttgaaccaa gaagaaaaga agacccccag
     1681 caaaccacca gcccagctgt cgccatctgt tcccaagaga cctaaattgc cagctccaac
     1741 tcgtacccca gccacagccc cagtccctgc ccgagcacag ggggcagtga caggcaaacc
     1801 ccgaggagaa ggcaccgagc ccagacgacc ccgagctggc ccagaggagc tggggaagat
     1861 ccttcagggt gtggtagtgg tgctgagtgg cttccagaac cccttccgct ccgagctgcg
     1921 agataaggcc ctagagcttg gggccaagta tcggccagac tggacccggg acagcacgca
     1981 cctcatctgt gcctttgcca acacccccaa gtacagccag gtcctaggcc tgggaggccg
     2041 catcgtgcgt aaggagtggg tgctggactg tcaccgcatg cgtcggcggc tgccctccca
     2101 gaggtacctc atggcagggc caggttccag cagtgaggag gatgaggcct ctcacagcgg
     2161 tggcagcgga gatgaagccc ccaagcttcc tcagaagcaa ccccagacca aaaccaagcc
     2221 cactcaggca gctggaccca gctcacccca gaagccccca acccctgaag agaccaaagc
     2281 agcctcacca gtgctccagg aagatataga cattgagggg gtacagtcag aaggacagga
     2341 caatggggcg gaagattctg gggacacaga ggatgagctg aggagggtgg cagagcagaa
     2401 ggaacacaga ctgccccctg gccaggagga gaatggggaa gacccgtatg caggctccac
     2461 ggatgagaac acggacagtg aggaacacca ggagcctcct gatctgccag tccctgagct
     2521 cccagatttc ttccagggca agcacttctt tctttacggg gagttccctg gggacgagcg
     2581 gcggaaactc atccgatacg tcacagcctt caatggggag ctcgaggact atatgagtga
     2641 ccgggttcag tttgtgatca cagcacagga atgggatccc agctttgagg aggccctgat
     2701 ggacaacccc tccctggcat tcgttcgtcc ccgatggatc tacagttgca atgagaagca
     2761 gaagttactt cctcaccagc tctatggggt ggtgccgcaa gccggatccg gcggtggagg
     2821 aagcggcgga ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2881 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2941 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3001 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3061 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     3121 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3181 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3241 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3301 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3361 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3421 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3481 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3541 ggacgagctg tacaagtagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     3601 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3661 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3721 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3781 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3841 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4021 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4081 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4201 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4261 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4561 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4621 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     4681 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4741 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4801 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4861 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4921 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4981 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     5041 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5101 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5161 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5221 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5281 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5341 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5401 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5461 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5521 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5581 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5641 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5701 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5761 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5821 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     5881 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5941 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6001 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6061 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6661 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7021 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7141 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7321 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7381 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7441 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7861 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7921 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7981 aagtgccacc tgacgtc
//
