LOCUS       pcDNA3.1-WWP1(human)    8209 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023576
VERSION     V023576
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3673
                     /label="WWP1(NM_007013)"
                     /note="WWP1(NM_007013)"
                     /gene="WWP1"
     polyA_signal    3776..4000
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4046..4474
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4488..4817
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4884..5678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5852..5973
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6022..6038)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6046..6062
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6070..6100)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6115..6136
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6424..7009)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7180..8040)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8041..8145)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gccactgctt caccaaggtc tgatactagt aataaccaca gtggaaggtt
      961 gcagttacag gtaactgttt ctagtgccaa acttaaaaga aaaaagaact ggttcggaac
     1021 agcaatatat acagaagtag ttgtagatgg agaaattacg aaaacagcaa aatccagtag
     1081 ttcttctaat ccaaaatggg atgaacagct aactgtaaat gttacgccac agactacatt
     1141 ggaatttcaa gtttggagcc atcgcacttt aaaagcagat gctttattag gaaaagcaac
     1201 gatagatttg aaacaagctc tgttgataca caatagaaaa ttggaaagag tgaaagaaca
     1261 attaaaactt tccttggaaa acaagaatgg catagcacaa actggtgaat tgacagttgt
     1321 gcttgatgga ttggtgattg agcaagaaaa tataacaaac tgcagctcat ctccaaccat
     1381 agaaatacag gaaaatggtg atgccttaca tgaaaatgga gagccttcag caaggacaac
     1441 tgccaggttg gctgttgaag gcacgaatgg aatagataat catgtaccta caagcactct
     1501 agtccaaaac tcatgctgct cgtatgtagt taatggagac aacacacctt catctccgtc
     1561 tcaggttgct gccagaccca aaaatacacc agctccaaaa ccactcgcat ctgagcctgc
     1621 cgatgacact gttaatggag aatcatcctc atttgcacca actgataatg cgtctgtcac
     1681 gggtactcca gtagtgtctg aagaaaatgc cttgtctcca aattgcacta gtactactgt
     1741 tgaagatcct ccagttcaag aaatactgac ttcctcagaa aacaatgaat gtattccttc
     1801 taccagtgca gaattggaat ctgaagctag aagtatatta gagcctgaca cctctaattc
     1861 tagaagtagt tctgcttttg aagcagccaa atcaagacag ccagatgggt gtatggatcc
     1921 tgtacggcag cagtctggga atgccaacac agaaaccttg ccatcagggt gggaacaaag
     1981 aaaagatcct catggtagaa cctattatgt ggatcataat actcgaacta ccacatggga
     2041 gagaccacaa cctttacctc caggttggga aagaagagtt gatgatcgta gaagagttta
     2101 ttatgtggat cataacacca gaacaacaac gtggcagcgg cctaccatgg aatctgtccg
     2161 aaattttgaa cagtggcaat ctcagcggaa ccaattgcag ggagctatgc aacagtttaa
     2221 ccaacgatac ctctattcgg cttcaatgtt agctgcagaa aatgaccctt atggaccttt
     2281 gccaccaggc tgggaaaaaa gagtggattc aacagacagg gtttactttg tgaatcataa
     2341 cacaaaaaca acccagtggg aagatccaag aactcaaggc ttacagaatg aagaacccct
     2401 gccagaaggc tgggaaatta gatatactcg tgaaggtgta aggtactttg ttgatcataa
     2461 cacaagaaca acaacattca aagatcctcg caatgggaag tcatctgtaa ctaaaggtgg
     2521 tccacaaatt gcttatgaac gcggctttag gtggaagctt gctcacttcc gttatttgtg
     2581 ccagtctaat gcactaccta gtcatgtaaa gatcaatgtg tcccggcaga cattgtttga
     2641 agattccttc caacagatta tggcattaaa accctatgac ttgaggaggc gcttatatgt
     2701 aatatttaga ggagaagaag gacttgatta tggtggccta gcgagagaat ggtttttctt
     2761 gctttcacat gaagttttga acccaatgta ttgcttattt gagtatgcgg gcaagaacaa
     2821 ctattgtctg cagataaatc cagcatcaac cattaatcca gaccatcttt catacttctg
     2881 tttcattggt cgttttattg ccatggcact atttcatgga aagtttatcg atactggttt
     2941 ctctttacca ttctacaagc gtatgttaag taaaaaactt actattaagg atttggaatc
     3001 tattgatact gaattttata actcccttat ctggataaga gataacaaca ttgaagaatg
     3061 tggcttagaa atgtactttt ctgttgacat ggagattttg ggaaaagtta cttcacatga
     3121 cctgaagttg ggaggttcca atattctggt gactgaggag aacaaagatg aatatattgg
     3181 tttaatgaca gaatggcgtt tttctcgagg agtacaagaa cagaccaaag ctttccttga
     3241 tggttttaat gaagttgttc ctcttcagtg gctacagtac ttcgatgaaa aagaattaga
     3301 ggttatgttg tgtggcatgc aggaggttga cttggcagat tggcagagaa atactgttta
     3361 tcgacattat acaagaaaca gcaagcaaat catttggttt tggcagtttg tgaaagagac
     3421 agacaatgaa gtaagaatgc gactattgca gttcgtcact ggaacctgcc gtttacctct
     3481 aggaggattt gctgagctca tgggaagtaa tgggcctcaa aagttttgca ttgaaaaagt
     3541 tggcaaagac acttggttac caagaagcca tacatgtttt aatcgcttgg atctaccacc
     3601 atataagagt tatgaacaac taaaggaaaa acttcttttt gcaatagaag agacagaggg
     3661 atttggacaa gaatgatgat tcaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3721 aaaaaaaaaa aactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3781 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3841 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3901 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3961 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4021 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4081 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4141 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4201 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4261 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4321 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4381 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4441 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4561 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4621 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4741 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4801 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4861 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4921 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4981 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5041 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5101 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5161 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5221 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5281 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5341 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5401 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5461 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5521 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5581 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5641 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5701 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5761 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5821 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5881 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5941 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6001 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6061 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6121 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6181 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6841 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8161 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
