LOCUS       pcDNA3.1-USP7(human)    8693 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018603
VERSION     V018603
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4213
                     /label="USP7(NM_003470)"
                     /note="USP7(NM_003470)"
                     /gene="USP7"
     polyA_signal    4260..4484
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4530..4958
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4972..5301
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5368..6162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6336..6457
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6506..6522)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6530..6546
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6554..6584)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6599..6620
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6908..7493)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7664..8524)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8525..8629)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aaccaccagc agcagcagca gcagcagaaa gcgggcgagc agcagttgag
      961 cgagcccgag gacatggaga tggaagcggg agatacagat gacccaccaa gaattactca
     1021 gaaccctgtg atcaatggga atgtggccct gagtgatgga cacaacaccg cggaggagga
     1081 catggaggat gacaccagtt ggcgctccga ggcaaccttt cagttcactg tggagcgctt
     1141 cagcagactg agtgagtcgg tccttagccc tccgtgtttt gtgcgaaatc tgccatggaa
     1201 gattatggtg atgccacgct tttatccaga cagaccacac caaaaaagcg taggattctt
     1261 tctccagtgc aatgctgaat ctgattccac gtcatggtct tgccatgcac aagcagtgct
     1321 gaagataata aattacagag atgatgaaaa gtcgttcagt cgtcgtatta gtcatttgtt
     1381 cttccataaa gaaaatgatt ggggattttc caattttatg gcctggagtg aagtgaccga
     1441 tcctgagaaa ggatttatag atgatgacaa agttaccttt gaagtctttg tacaggcgga
     1501 tgctccccat ggagttgcgt gggattcaaa gaagcacaca ggctacgtcg gcttaaagaa
     1561 tcagggagcg acttgttaca tgaacagcct gctacagacg ttatttttca cgaatcagct
     1621 acgaaaggct gtgtacatga tgccaaccga gggggatgat tcgtctaaaa gcgtcccttt
     1681 agcattacaa agagtgttct atgaattaca gcatagtgat aaacctgtag gaacaaaaaa
     1741 gttaacaaag tcatttgggt gggaaacttt agatagcttc atgcaacatg atgttcagga
     1801 gctttgtcga gtgttgctcg ataatgtgga aaataagatg aaaggcacct gtgtagaggg
     1861 caccataccc aaattattcc gcggcaaaat ggtgtcctat atccagtgta aagaagtaga
     1921 ctatcggtct gatagaagag aagattatta tgatatccag ctaagtatca aaggaaagaa
     1981 aaatatattt gaatcatttg tggattatgt ggcagtagaa cagctcgatg gggacaataa
     2041 atacgacgct ggggaacatg gcttacagga agcagagaaa ggtgtgaaat tcctaacatt
     2101 gccaccagtg ttacatctac aactgatgag atttatgtat gaccctcaga cggaccaaaa
     2161 tatcaagatc aatgataggt ttgaattccc agagcagtta ccacttgatg aatttttgca
     2221 aaaaacagat cctaaggacc ctgcaaatta tattcttcat gcagtcctgg ttcatagtgg
     2281 agataatcat ggtggacatt atgtggttta tctaaacccc aaaggggatg gcaaatggtg
     2341 taaatttgat gacgacgtgg tgtcaaggtg tactaaagag gaagcaattg agcacaatta
     2401 tgggggtcac gatgacgacc tgtctgttcg acactgcact aatgcttaca tgttagtcta
     2461 catcagggaa tcaaaactga gtgaagtttt acaggcggtc accgaccatg atattcctca
     2521 gcagttggtg gagcgattac aagaagagaa aaggatcgag gctcagaagc ggaaggagcg
     2581 gcaggaagcc catctctata tgcaagtgca gatagtcgca gaggaccagt tttgtggcca
     2641 ccaagggaat gacatgtacg atgaagaaaa agtgaaatac actgtgttca aagtattgaa
     2701 gaactcctcg cttgctgagt ttgttcagag cctctctcag accatgggat ttccacaaga
     2761 tcaaattcga ttgtggccca tgcaagcaag gagtaatgga acaaaacgac cagcaatgtt
     2821 agataatgaa gccgacggca ataaaacaat gattgagctc agtgataatg aaaacccttg
     2881 gacaatattc ctggaaacag ttgatcccga gctggctgct agtggagcga ccttacccaa
     2941 gtttgataaa gatcatgatg taatgttatt tttgaagatg tatgatccca aaacgcggag
     3001 cttgaattac tgtgggcata tctacacacc aatatcctgt aaaatacgtg acttgctccc
     3061 agttatgtgt gacagagcag gatttattca agatactagc cttatcctct atgaggaagt
     3121 taaaccgaat ttaacagaga gaattcagga ctatgacgtg tctcttgata aagcccttga
     3181 tgaactaatg gatggtgaca tcatagtatt tcagaaggat gaccctgaaa atgataacag
     3241 tgaattaccc accgcaaagg agtatttccg agatctctac caccgcgttg atgtcatttt
     3301 ctgtgataaa acaatcccta atgatcctgg atttgtggtt acgttatcaa atagaatgaa
     3361 ttattttcag gttgcaaaga cagttgcaca gaggctcaac acagatccaa tgttgctgca
     3421 gtttttcaag tctcaaggtt atagggatgg cccaggtaat cctcttagac ataattatga
     3481 aggtacttta agagatcttc tacagttctt caagcctaga caacctaaga aactttacta
     3541 tcagcagctt aagatgaaaa tcacagactt tgagaacagg cgaagtttta aatgtatatg
     3601 gttaaacagc caatttaggg aagaggaaat aacactatat ccagacaagc atgggtgtgt
     3661 ccgggacctg ttagaagaat gtaaaaaggc cgtggagctt ggggagaaag catcagggaa
     3721 acttaggctg ctagaaattg taagctacaa aatcattggt gttcatcaag aagatgaact
     3781 attagaatgt ttatctcctg caacgagccg gacgtttcga atagaggaaa tccctttgga
     3841 ccaggtggac atagacaaag agaatgagat gcttgtcaca gtggcgcatt tccacaaaga
     3901 ggtcttcgga acgttcggaa tcccgttttt gctgaggata caccagggcg agcattttcg
     3961 agaagtgatg aagcgaatcc agagcctgct ggacatccag gagaaggagt ttgagaagtt
     4021 taaatttgca attgtaatga tgggccgaca ccagtacata aatgaagacg agtatgaagt
     4081 aaatttgaaa gactttgagc cacagcccgg taatatgtct catcctcggc cttggctagg
     4141 gctcgaccac ttcaacaaag ccccaaagag gagtcgctac acttaccttg aaaaggccat
     4201 taaaatccat aactgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     4261 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4321 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4381 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4441 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4501 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4981 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5341 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5401 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5461 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5521 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     5581 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5641 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5701 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5761 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5821 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5881 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5941 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6001 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6061 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6121 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     6181 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     6241 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     6301 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     6361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     6481 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7441 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8641 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
