LOCUS       V028882                 8170 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028882
VERSION     V028882
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3690
                     /label="USP11(NM_001371072)"
                     /note="USP11(NM_001371072)"
                     /gene="USP11"
     polyA_signal    3737..3961
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4007..4435
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4449..4778
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4845..5639
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5813..5934
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5983..5999)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6007..6023
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6031..6061)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6076..6097
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6385..6970)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7141..8001)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8002..8106)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcgacgg tcgcagcaaa tccagctgct
      961 gctgcggcgg ctgtggcggc ggcagcggcg gtgactgagg atagagagcc acagcacgag
     1021 gagctgccag gcctggacag ccagtggcgc cagatagaaa acggcgagag tgggcgagaa
     1081 cgtccactgc gggccggcga aagctggttc cttgtggaga agcactggta taagcagtgg
     1141 gaggcatacg tgcagggagg ggaccaggac tccagcacct tccctggctg catcaacaat
     1201 gccacactct ttcaagatga gataaactgg cgcctcaagg agggactggt ggaaggcgag
     1261 gattatgtgc tgctcccagc agctgcttgg cattacctgg tcagctggta tggtctagag
     1321 catggccagc cacccattga acgcaaggtc atagagctgc ccaacatcca gaaggtcgaa
     1381 gtgtacccag tagaactgct gcttgtccgg cacaatgatt tgggcaaatc tcacactgtt
     1441 cagttcagcc ataccgattc tattggccta gtattgcgca cagctcggga gcggtttctg
     1501 gtggagcccc aggaagacac tcggctttgg gccaagaact cagaaggctc tttggatagg
     1561 ttgtatgaca cacacatcac ggttctcgat gcggcccttg agactgggca gttgatcatc
     1621 atggagaccc gcaagaaaga tggcacttgg cccagcgcac agctgcatgt catgaacaac
     1681 aacatgtcgg aagaggatga ggacttcaag ggtcagccag gcatctgtgg cctcaccaat
     1741 ctgggcaaca cgtgcttcat gaactcggcc ctgcagtgcc tcagcaatgt gccacagctc
     1801 accgagtact tcctcaacaa ctgctacctg gaggagctca acttccgcaa cccactgggc
     1861 atgaagggtg agatcgcaga ggcctatgca gacctggtga agcaggcgtg gtctggccac
     1921 caccgctcca ttgtgccaca tgtgttcaag aacaaggttg gccattttgc atcccaattt
     1981 ctgggctacc agcagcatga ctctcaggag ctgctgtcat tcctcctgga cgggctgcat
     2041 gaggacctta atcgggtgaa gaagaaggag tatgtggagc tgtgcgatgc tgctgggcga
     2101 ccggatcagg aggtggcaca ggaggcatgg caaaaccaca aacggcggaa cgattctgtg
     2161 atcgtggaca ctttccacgg cctcttcaag tccacgctgg tgtgccccga ttgtggcaat
     2221 gtatctgtga ccttcgaccc cttctgctac ctcagtgttc cactgcctat cagccacaag
     2281 agggtcttgg aggtcttctt tatccccatg gatccgcgcc gcaagccaga gcagcaccgg
     2341 ctcgtggtcc ccaagaaagg caagatctcg gatctatgtg tggctctgtc caaacacacg
     2401 ggcatctcgc cagagaggat gatggtggct gatgtcttca gtcaccgctt ctataagctc
     2461 tatcagctag aggagcctct gagcagcatc ttggaccgtg atgatatctt cgtctatgag
     2521 gtgtcaggtc gcattgaggc cattgagggc tcaagagagg acatcgtggt tcctgtctac
     2581 ctgcgggagc gcacccctgc ccgtgactac aacaactcct actacggcct gatgcttttt
     2641 ggacaccccc tcctggtatc agtgccccgg gaccgcttca cctgggaggg cctgtataac
     2701 gtcctgatgt accggctctc acgctacgtg accaaaccca actcagatga tgaggacgat
     2761 ggggatgaga aagaagatga cgaggaggat aaagatgacg tccctgggcc ctcaactggg
     2821 ggcagcctcc gagaccctga gccagagcag gctgggccca gctctggagt cacgaacagg
     2881 tgcccgttcc tcctggacaa ttgccttggc acatctcagt ggcccccaag gcgacgacgc
     2941 aagcagctgt tcaccctgca gacggtgaac tccaatggga ccagcgaccg cacaacctcc
     3001 cctgaagaag tccatgccca gccgtacatt gctatcgact gggagccaga gatgaagaag
     3061 cgttactatg acgaggtaga ggctgagggc tacgtgaagc atgactgcgt cgggtacgtg
     3121 atgaagaagg ctcccgtgcg gctgcaggag tgcattgagc tcttcaccac tgtggagacc
     3181 ctggagaagg aaaacccctg gtactgccct tcctgcaagc agcaccagct ggcaaccaag
     3241 aagctggacc tgtggatgct gccggagatt ctcatcatcc acctgaaacg cttttcctac
     3301 accaagttct cccgagagaa gctggacacc ctcgtggagt ttcctatccg ggacctggac
     3361 ttctctgagt ttgtcatcca gccacagaat gagtcgaatc cggagctgta caaatatgac
     3421 ctcatcgcgg tttccaacca ttatgggggc atgcgtgatg gacactacac aacatttgcc
     3481 tgcaacaagg acagcggcca gtggcactac tttgatgaca acagcgtctc ccctgtcaat
     3541 gagaatcaga tcgagtccaa ggcagcctat gtcctcttct accaacgcca ggacgtggcg
     3601 cgacgcctgc tgtccccggc cggctcatct ggcgccccag cctcccctgc ctgcagctcc
     3661 ccacccagct ctgagttcat ggatgttaat tgactcgagt ctagagggcc cgtttaaacc
     3721 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3781 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3841 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3901 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3961 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4081 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4201 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4321 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4381 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     4441 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4501 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4621 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4681 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     4741 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     4801 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4861 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4921 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4981 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5041 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5101 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5161 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5221 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5281 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5341 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     5401 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5461 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5521 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5581 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     5641 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     5701 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5761 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     5821 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5881 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5941 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6001 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6061 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6121 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6181 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6301 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6421 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6481 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6541 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6601 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6661 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6721 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6781 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6841 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6901 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6961 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7021 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7081 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7141 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7201 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7261 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7321 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7381 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7441 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7501 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7561 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7621 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7681 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7741 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7801 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7861 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7921 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7981 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8041 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8101 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8161 cccctatggt
//
