LOCUS       V035838                 7833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V035838
VERSION     V035838
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..3348
                     /label="USP10(NM_005153)"
                     /note="USP10(NM_005153)"
                     /gene="USP10"
     CDS             3370..3399
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3428..3652
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3698..4126
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4140..4469
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4536..5330
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5506..5627
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5676..5692)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5700..5716
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5724..5754)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5769..5790
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6078..6666)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6837..7697)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7698..7802)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggcc
      961 ctccacagcc cgcagtatat ttttggagat tttagccctg atgaattcaa tcaattcttt
     1021 gtgactcctc gatcttcagt tgagcttcct ccatacagtg gaacagttct gtgtggcaca
     1081 caggctgtgg ataaactacc tgatggacaa gaatatcaga gaattgagtt tggtgtcgat
     1141 gaagtcattg aacccagtga cactttgccg agaaccccca gctacagtat ttcaagcaca
     1201 ctgaaccctc aggcccctga atttattctc ggttgtacag cttccaaaat aacccctgat
     1261 ggtatcacta aagaagcaag ctatggctcc atcgactgcc agtacccagg ctctgccctc
     1321 gctttggatg gaagttctaa tgtggaggcg gaagttttgg aaaatgatgg tgtctcaggt
     1381 ggtcttggac aaagggagcg taaaaagaag aaaaagcggc cacctggata ttacagctat
     1441 ttgaaagatg gtggcgatga tagtatctcc acagaagccc tggtcaatgg ccatgccaat
     1501 tcagcagtcc cgaacagtgt cagtgcagag gatgcagaat ttatgggtga catgcccccg
     1561 tcagttacgc ccaggacttg taacagcccc cagaactcca cagactctgt cagtgacatt
     1621 gtgcctgaca gtcctttccc cggagcactc ggcagtgaca ccaggactgc agggcagcca
     1681 gaggggggcc ccggggctga ttttggtcag tcctgcttcc ctgcagaggc tggcagagac
     1741 accctgtcaa ggacagctgg ggctcagccc tgcgttggta ccgatactac tgaaaacctt
     1801 ggagttgcta atggacaaat acttgaatcc tcgggtgagg gcacagctac caacggggtg
     1861 gagttgcaca ccacggaaag catagacttg gacccaacca aacccgagag tgcatcacct
     1921 cctgctgacg gcacgggctc tgcatcaggc acccttcctg tcagccagcc caagtcctgg
     1981 gccagcctct ttcatgattc taagccctct tcctcctcgc cggtggccta tgtggaaact
     2041 aagtattccc ctcccgccat atctcccctg gtttctgaaa agcaggttga agtcaaagaa
     2101 gggcttgttc cggtttcaga ggatcctgta gccataaaga ttgcagagtt gctggagaat
     2161 gtaaccctaa tccataaacc agtgtcgttg caaccccgtg ggctgatcaa taaagggaac
     2221 tggtgctaca ttaatgctac actgcaggca ttggttgctt gcccgccgat gtaccacctg
     2281 atgaagttca ttcctctgta ttccaaagtg caaaggcctt gtacgtcaac acccatgata
     2341 gacagctttg ttcggctaat gaatgagttc actaatatgc cagtacctcc aaaaccccga
     2401 caagctcttg gagataaaat cgtgagggat attcgccctg gagctgcctt tgagcccaca
     2461 tatatttaca gactcctgac agttaacaag tcaagcctgt ctgaaaaggg tcgacaagaa
     2521 gatgctgagg aatacttagg cttcattcta aatggacttc atgaggaaat gttgaaccta
     2581 aagaagcttc tctcaccaag taatgaaaaa cttacgattt ccaacggccc caaaaaccac
     2641 tcggtcaatg aagaagagca ggaagaacaa ggtgaaggaa gcgaggatga atgggaacaa
     2701 gtgggccccc ggaacaagac ttccgtcacc cgccaggcgg attttgttca gactccaatc
     2761 accggcattt ttggtggaca catcaggtct gtggtttacc agcagagttc aaaagaatct
     2821 gccactttgc agccattttt cacgttgcag ttggatatcc agtcagacaa gatacgcaca
     2881 gtccaggatg cactggagag cttggtggca agagaatctg tccaaggtta taccacaaaa
     2941 accaaacaag aggttgagat aagtcgaaga gtgactctgg aaaaactccc tcctgtcctc
     3001 gtgctgcacc tgaaacgatt cgtttatgag aagactggtg ggtgccagaa gcttatcaaa
     3061 aatattgaat atcctgtgga cttggaaatt agtaaagaac tgctttctcc aggggttaaa
     3121 aataagaatt ttaaatgcca ccgaacctat cggctctttg cagtggtcta ccatcacggc
     3181 aacagtgcga cgggcggcca ttacactaca gacgtcttcc agatcggtct gaatggctgg
     3241 ctgcgcatcg atgaccagac agtcaaggtg atcaaccagt accaggtggt gaaaccaact
     3301 gctgaacgca cagcctacct cctgtattac cgccgagtgg acctgctgct cgagtctaga
     3361 gggcccttcg aacaaaaact catctcagaa gaggatctgt gagtttaaac ccgctgatca
     3421 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3481 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3541 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3601 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3661 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3721 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3781 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3841 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3901 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3961 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4021 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4081 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4381 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4441 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4501 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4561 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4621 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4681 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     4741 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4801 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4861 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4921 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4981 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5041 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5101 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5161 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5221 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5281 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5341 ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5401 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5461 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     5521 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5581 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     5641 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5701 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5761 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5821 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5881 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5941 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6001 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6061 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6121 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6181 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6241 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6301 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     6361 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6421 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6481 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6541 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6601 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6661 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6721 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6781 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6841 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6901 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6961 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7021 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7081 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7141 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7201 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7261 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7321 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     7381 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7441 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7501 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7561 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7621 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7681 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7741 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7801 cgcacatttc cccgaaaagt gccacctgac gtc
//
