LOCUS       V044511                12269 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044511
VERSION     V044511
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..1639
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1676..7759
                     /label="Trpm6(NM_153417)"
                     /note="Trpm6(NM_153417)"
                     /gene="Trpm6"
     CDS             7766..7831
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    7869..8093
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      8139..8567
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8581..8910
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8977..9771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9945..10066
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(10115..10131)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10139..10155
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10163..10193)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10208..10229
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10517..11102)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11273..12133)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12134..12238)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1621 catggacgag ctgtacaagg gcggtggagg cagtggtgga ggcggtagcg aattcatgca
     1681 ggtcaagaaa tcctggatcg aaggggtatt ctacaagaga gagtgtaaca aattcatacc
     1741 cagctcaaaa gaccctcaca gatgcactcc aggctgccaa atctgccaca atttagtcag
     1801 gtgttactgt ggccggctga ttgaagaaca ccatgggctg gatcgtgcct ggaacctctc
     1861 agtcactgag ggccatgggg atgagcagtg gtctgtggag aagcacacag tgaaaagccc
     1921 tacagatacc ttcggcacca ttaatttcca agatggagaa catatccacc actccaagta
     1981 cattagaact tcttgggata caaaatcgga tcatctgttg cacttaatgc tgaaagagtg
     2041 gaatatggaa ctgccaaagc ttgtgatctc cgtccatggg ggtcttcaga acttcaagat
     2101 atcctccaag ctcaaagaga ccttcagcca gggtctggtc aaagctgcag agaccacagg
     2161 agcatggata attacagaag gcataaactc aggagtgtcc aagcacgttg gggatgccct
     2221 aaaagcccat tcctctaagt ctttgaggaa aatctggaca gttggaatcc ctccttgggg
     2281 agtcattgag aaccagagag agctggtcgg aaaagatgtg gtgtgtatgt accagacact
     2341 gggtaaccca ctcagcaagc tcaccacgct aaactgtatg cactctcact tcatcctgtg
     2401 tgatgatggg actgtgggca tgtatggaaa tgaagagaag ctcaggcgga acctggagaa
     2461 gcatctctca atgcagaaga tacacacttg ctcaagacaa ggtgtgcctg tggtggggct
     2521 ggtgatggaa ggaggtccca atgtcatcct ctgggtatgg gagaccgtca agaacaagga
     2581 gcccgtggtg gtgtgcgagg ggacaggcag agctgctgac ctcttggcct tcacttacaa
     2641 acatttggag gatggaggga ttctacgccc tcaggtgaaa gaagagctct tctgtttgat
     2701 tcagaacatg ttcaacttta gccttagaca gtctaagcac cttttccaaa ttctaatgga
     2761 gtgcatggta cacaaggatt ctatcaccat atttgatgct gattctgaag agcaccaaga
     2821 tcttgatttg gccatcctca cggctctact gaagggtaca agcttatcaa tatcagaaca
     2881 attaaatctg gcaatggctt gggatagaat ggacattgcc aagaaacata tcctaactta
     2941 tggacaacat tggaagcctg gtgctctaga acaagcaatg ctagatgctt tagtgatgga
     3001 tcgagtggac tttgtgaaac ttttgataga gaatggggtg aacctccacc gcttcctcac
     3061 catccctcgg ctggaagagc tctataacac aaaacaagga ccaactaata aatttttgcg
     3121 tcatcttgtc caagacgtaa agcagcacac ccttctctcg agctatagaa taacactcat
     3181 tgacattggg ctggtaatag agtacctcat tggtggagca tatcggagca gctacactag
     3241 gaaaagtttc agaattctct acaacaacct ctacagaaaa cacaagagtg tgagcagctt
     3301 tgctcagggc ctctcccagc actctcttca tcagagacat tcactgagaa atagaaagga
     3361 gtcttctgaa agcacactgc actcccagtt cttcaggacg gcccagccct acaaatccaa
     3421 ggagaagcct gaggactctc aaaaatcaaa gaagaagtcc aaggaaagac aaagcctgtc
     3481 agaggaaccc gaggctgctg gctttatcta cccatacaat gaccttctgg tgtgggcggt
     3541 gctcatgaag aggcagaata tggccatgtt cttctggcag catggagagg aggccacagt
     3601 caaggcggtc attgctagta tcctctacag agccatggcc cgggaagcca aggagagcaa
     3661 catggtggat gacacttcag aagagttgaa aaattactca gagcagtttg gccagctcgc
     3721 cctggatgta ttagagaaag ccttcaagca gaacgagccg atggccatga agctgctgac
     3781 ctatgagctc aagaattgga gtaattccac ctgcttgaaa ctggcggtct ctggaggtct
     3841 gcgccctttt gtgtcgcatt cttgtaccca aatgctgcta acagacatgt ggatggggcg
     3901 tctgaaaatg agaaaaaatt cctggctgaa gataatcatc agtatcctct taccacccat
     3961 gattttgaca ttggaattta aaagcaaagc agagatgtca catgtcccac agtcccagga
     4021 tttccagttt acatggaatt acagtgacca gggcctgagt aacaccaaag aaagtgcttg
     4081 tgtgaaagac tatgatttgg aaagaggccc tgatgagaaa ccggatgaac ctctgcattt
     4141 agacttaaga aatgtgcccc agagcctccc ctggacccgg agagtctacg agttctacag
     4201 cgctccgttt gtcaagtttt ggttctacac gatggcttat ttggcattcc tcatgctgtt
     4261 cacttacaca gtgttggtgg agatgcagcc ccaacccagc gtgcacgaat ggcttgtcat
     4321 catctacatc ttcaccaatg ctattgagaa ggtcagggag atctgcattt cagaaccaag
     4381 caagtttaag cagaaggtga agatgtggct cagtgagtac tggaacctca tggaaactgt
     4441 ggctattggc ctgtttgctg ttggctttgg cctacggtgg gggcatccac ctttgcaaac
     4501 tgcagggaga ctcatctact gcatagacat catattttgg ttttctcggc tcatggactt
     4561 ctttgctgtg aatcaacatg caggtccata tgtgaccatg attgccaaaa tggcagcaaa
     4621 catgttctac attgtgatca tcatggccat tgtcctgctg agctttggcg tggcgcgcaa
     4681 agctatcctc tcaccgaagg agcctccttc atggcgcctg gctcgtgaca tcgtctttga
     4741 gccgtattgg atgatgtatg gagaagtcta tgcttcagat atagatgttt gttcaaatga
     4801 gacttcttgc cctccgggtt ctttcctgac tccattcctg caagctgtct acctctttgt
     4861 gcaatacatc atcatggtga accttctgat tgcctgtttc aacaacattt acttagatat
     4921 aaaatccatc tcgaacaagc tctggaaata caaccgctat cgctacatca tgacctacca
     4981 ccagaagccc tggctgcctc ctccctttat cctgctgaat cacctgtgtc tcctcctccg
     5041 aggtctgtgc tgtcgcccag ccccacagga ccaggaagag ggggacggag ggctaaagct
     5101 ctatctcact aaggatgact tgaaaaagct tcatgatttt gaagagcagt gtgtggaaaa
     5161 atatttccac gagaagacgg aaggtctgaa ttgtagtttt gaggaacaaa ttcgaatgac
     5221 atcagaaagg gtatcagaga tgtttttcca actgaaagaa atgaatgaaa aagtatcttt
     5281 tataaaggac tccctactat ccctggacag ccaggtggga caccttcagg acctctcggc
     5341 cataactgtt gacaccttaa aggtcctttc tgctgttgac accttgcaag aagatgagat
     5401 tcttttggcc aacagaaagc attctacttg cagaaaacgt ccccacagct ggaccaatgt
     5461 catctgtgca aaggttctaa gtgacatgga aagctgtggg aagaagaaac ttcagtatta
     5521 tagcatgccc ccttctttgc tgaggagcct cgctagaagt cagcttcccc caagtgtgca
     5581 gaggggtgca cttgtagagg ttacgcacag taagagagag gcctcacatg taagagaaga
     5641 gcaggaggaa cgagaaatgg aacagaggac aacggcttct gggatttccc atgtcagaca
     5701 agcacactct aagtatggcc agtttctcct ggttccatct tctggaaagc aagttccttt
     5761 gtctttggaa accccgccac atcttttcag atcatctgag gaggcaggta tagatggtct
     5821 ggtattggag catatacacc aaagtgatct caccacccac ttgcctcagc agacccctgc
     5881 tgcctcccac caggcattgg tagcggaaca caaagaccaa catgaggcag ttacacagat
     5941 gtcagacaaa cctgccaagg cagaacaaga cttgctcgca ttcagtggca cacctgctcc
     6001 catgacagtg acttctcttc cttcgagagc catcagcatg caagatgaag gtggatatgt
     6061 aaactgggca ttttcagaaa atgatgaaac aggtgtgttt agctttaaaa aaaagtggaa
     6121 aacttgcttg gcctccactt gcaatagtga ctccaatcct ggtggagact acttcctgca
     6181 cactggaggt agatccggac tggataactc aagaagactg gcccagagtt gtgagtgccc
     6241 agcaggaccg tggacccagg caagaagatc cttttggatc aatcctctct gcagagataa
     6301 agctctcatt aagagtcata gttttagatt ccacaaggag gagaaactga ggaagacctg
     6361 gaagaacaac agtcattcaa aatccttaga gaccaggtcc acatggctca aagcaaaact
     6421 gctaaccaag acccggagcc tgtcaaagaa gaagaggaag acccaagggc tccaggtgcc
     6481 ggtaataaca gtcaatgcct gctatcaaag tgaccagttg aatgcagagc caggagaaac
     6541 taacaccact gaagagttca gcaagaaatg gctctctgta tccaacttca gccagatagg
     6601 tttagagcct tacatatacc agaaaatgaa aatgaaggaa atcaagcgac atactacaca
     6661 agccagtgac cacctaaggc agccacaaga gaaccgagat aaaaccccca catggaattc
     6721 tgggagcacc agcctcagca ggagttttct aacaagaagt ccaaatgaag ttcacaagat
     6781 ctcaacctcc ttaaaaagcc ctcaagagcc tcaccaccat tattcagcca ttgaaaggaa
     6841 taatttaatg agactgtctc agaccatacc gtttacacca atccagctgt tcacaggaga
     6901 ggaagtgacc atctacaagc ttgaagaaag ttctcctctg accctggata agagcatgtc
     6961 ctcttggtcg cagcatggca gagctgccat gattcaggtg ctgtcacaag aggaaatgga
     7021 tgggggtctc cgcaaagcca tgcgagttat cagcacctgg tctgaggatg atgttctcaa
     7081 gcctgggcag gttttcattg tgaagtcttt tctccccgag gttgtgcaga cgtggtataa
     7141 aatcttccag gagagcactg tgcttcatct ttgccttagg gaaattcaac agcaaagagc
     7201 tgcccaaaaa ctcatctata cctttaacca agtaaaacca caaaccattc cctatacacc
     7261 aaggtttctc gaggtttcct tggtctactg ccattcagcc aaccaatggt taaccattga
     7321 gaagtacatg acaggggagt tccggaaata caataacaac aatggtgatg aaatagctcc
     7381 caccaatacc ctggaagaac tgatgttggc tttctctcac tggacctatg aatatacccg
     7441 gggagagctg ctggttttag atttgcaagg tgttggagaa aatttgacag atccgtctgt
     7501 tataaaaccg gaagacaaac aatcaagagg gatggtgttt ggaccggcca atttagggga
     7561 agatgcaatt agaagcttca ttgcaaaaca tcgctgcaac tcctgctgtg ggaagctcag
     7621 actgccggat ttaaaaagga atgattactc cctttcaaga acacactgca acttgggatt
     7681 tgggcaaacc attgaaccaa ctgaggagct tccagaaaga gacaaaaata gaagttccct
     7741 ggaagatcac acacgccttc tcgaggacta caaagaccat gacggtgatt ataaagatca
     7801 tgacatcgac tacaaggatg acgatgacaa gtagtgaggg cccgtttaaa cccgctgatc
     7861 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8041 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     8101 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8161 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8221 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8281 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8341 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8401 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8461 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8521 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8821 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8881 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8941 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     9001 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9061 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9121 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9181 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9241 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9301 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9361 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9421 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9481 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     9541 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9601 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9661 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9721 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     9781 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     9841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     9961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    10081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11041 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    11221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    11281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    11341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    11401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    11461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    11521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    12241 catttccccg aaaagtgcca cctgacgtc
//
