LOCUS       V026668                 7543 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026668
VERSION     V026668
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3036
                     /label="Tap2(NM_011530)"
                     /note="Tap2(NM_011530)"
                     /gene="Tap2"
     CDS             3043..3069
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3110..3334
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3380..3808
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3822..4151
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4218..5012
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5186..5307
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5356..5372)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5380..5396
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5404..5434)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5449..5470
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5758..6343)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6514..7374)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7375..7479)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcgctgt cctacctgag gccctgggtc
      961 tctctgctgc tggcggacat ggctttactt gggttgctac aaggatctct gggaaatctg
     1021 cttccccagg ggctgccagg actctggata gagggcaccc tgcgacttgg agtgctgtgg
     1081 ggactgctaa aagtgggaga gctgctggga cttgtgggga cccttctgcc cttgctctgc
     1141 cttgccactc ccctgttttt ctcgctaaga gctctggtgg gaggcaccgc gagcacctca
     1201 gtagtccgag tggcttctgc ctcttggggc tggctgctgg ctggctatgg ggctgttgcg
     1261 ctgagctggg ccgtgtgggc tgtgctgagc ccggctggag tccaggagaa ggaaccaggc
     1321 caggagaaca gaacactgat gaagcggttg ctgaagctgt ccaggccgga cctgcctttc
     1381 ctcatagctg ccttcttctt ccttgtggtg gctgtgtggg gggagacatt aatccctcgc
     1441 tattcgggtc gtgtaattga catcctggga ggtgatttcg accccgacgc ctttgcaagc
     1501 gccatctttt tcatgtgcct gttctctgtt gggagctcct tctctgcagg ctgtagagga
     1561 ggctccttcc tcttcaccat gtccaggatc aacctgcgga tacgagagca gcttttctca
     1621 tctttgttgc gccaagacct tggattcttc caggagacca agacagggga gctgaactcg
     1681 aggctgagct ctgacacctc tctgatgagc cgctggctcc ctttcaatgc caatatcctg
     1741 ctgcggagcc tggtgaaggt ggtggggctc tacttcttca tgctccaggt atcgccccga
     1801 ctcaccttcc tctccctgct ggacctgccc ctcacgatag cagctgagaa ggtgtacaac
     1861 ccccgccatc aggcggtgct aaaggagatc caggatgcag tggccaaggc ggggcaggtg
     1921 gtgcgcgagg cggtaggagg gctgcagact gtgcgaagct ttggggccga ggagcaggaa
     1981 gtcagccact acaaggaggc cctggagcga tgtagacagc tgtggtggcg ccgagacctg
     2041 gaaaaagacg tgtatctagt catacggagg gtgatggcct tgggcatgca ggtgctgatt
     2101 ctgaactgcg gcgtgcagca gattctggct ggagaggtca cccggggtgg cctgctctcc
     2161 ttcctgctgt accaggagga agtgggacaa tatgtccgga acctggttta catgtacggg
     2221 gatatgctga gcaacgtggg cgctgctgaa aaggtgtttt cctacctgga ccgaaagccg
     2281 aatctgcccc agcctgggat cctggcccct ccctggctgg aggggcgcgt ggaattccaa
     2341 gacgtctcct tttcgtatcc caggcgcccc gagaagcctg tgctccaggg tctgacgttc
     2401 accctgcatc ctggaacggt gacagcgttg gtgggaccca atggatcagg gaagagcacc
     2461 gtggccgccc tgctgcagaa cctgtaccag cccactgggg gccagctgct gctggatggc
     2521 gagcccctga ccgagtatga tcaccactac ctgcaccgcc aggtggttct ggtggggcag
     2581 gagcctgtgc tgttctcggg ttctgtcaag gacaatattg cctatggcct gagggactgt
     2641 gaggacgctc aagtgatggc agctgcccag gcggcctgtg cagacgactt cataggggaa
     2701 atgactaatg gaataaacac agaaatcggg gaaaaagggg gccagttagc tgtgggacag
     2761 aagcaacgtc tggccattgc ccgggccctt gtgcggaacc cacgggtcct catcctggat
     2821 gaggctacca gcgccctgga cgcccagtgt gaacaggccc tacagaactg gagatcgcag
     2881 ggggacagga cgatgctggt gattgcccac aggctgcaca cggttcagaa tgctgaccaa
     2941 gttctggtgc tcaagcaggg acgtctggtg gagcatgacc agctcaggga cggccaggat
     3001 gtctacgccc acctggtaca gcagcggctg gaggcactcg agtacccata cgacgtacca
     3061 gattacgctt agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     3121 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     3181 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3241 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3301 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3361 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3421 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3481 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3541 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3601 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3661 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3721 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3781 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3901 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4081 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4141 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     4201 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4261 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4321 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4381 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4441 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4501 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4561 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4621 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4681 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4741 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4801 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4861 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4921 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4981 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5041 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5101 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5161 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5281 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5341 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5401 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5461 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5521 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5581 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5641 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5701 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5761 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5821 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5881 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5941 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6001 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6061 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6121 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6181 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6241 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     6301 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6361 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6421 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6481 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6541 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6601 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6661 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6721 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6781 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6841 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6901 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6961 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7021 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7081 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     7141 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7201 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7261 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7321 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7381 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7441 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7501 acctgacgtc gacggatcgg gagatctccc gatcccctat ggt
//
