LOCUS       V026784                 7985 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026784
VERSION     V026784
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3424
                     /label="TRPV1(NM_080706)"
                     /note="TRPV1(NM_080706)"
                     /gene="TRPV1"
     CDS             3431..3457
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3461..3487
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3491..3517
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3552..3776
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3822..4250
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4264..4593
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4660..5454
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5628..5749
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5798..5814)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5822..5838
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5846..5876)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5891..5912
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6200..6785)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6956..7816)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7817..7921)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aagaaatgga gcagcacaga cttgggggca gctgcggacc cactccaaaa
      961 ggacacctgc ccagaccccc tggatggaga ccctaactcc aggccacctc cagccaagcc
     1021 ccagctctcc acggccaaga gccgcacccg gctctttggg aagggtgact cggaggaggc
     1081 tttcccggtg gattgccctc acgaggaagg tgagctggac tcctgcccga ccatcacagt
     1141 cagccctgtt atcaccatcc agaggccagg agacggcccc accggtgcca ggctgctgtc
     1201 ccaggactct gtcgccgcca gcaccgagaa gaccctcagg ctctatgatc gcaggagtat
     1261 ctttgaagcc gttgctcaga ataactgcca ggatctggag agcctgctgc tcttcctgca
     1321 gaagagcaag aagcacctca cagacaacga gttcaaagac cctgagacag ggaagacctg
     1381 tctgctgaaa gccatgctca acctgcacga cggacagaac accaccatcc ccctgctcct
     1441 ggagatcgcg cggcaaacgg acagcctgaa ggagcttgtc aacgccagct acacggacag
     1501 ctactacaag ggccagacag cactgcacat cgccatcgag agacgcaaca tggccctggt
     1561 gaccctcctg gtggagaacg gagcagacgt ccaggctgcg gcccatgggg acttctttaa
     1621 gaaaaccaaa gggcggcctg gattctactt cggtgaactg cccctgtccc tggccgcgtg
     1681 caccaaccag ctgggcatcg tgaagttcct gctgcagaac tcctggcaga cggccgacat
     1741 cagcgccagg gactcggtgg gcaacacggt gctgcacgcc ctggtggagg tggccgacaa
     1801 cacggccgac aacacgaagt ttgtgacgag catgtacaat gagattctga tgctgggggc
     1861 caaactgcac ccgacgctga agctggagga gctcaccaac aagaagggaa tgacgccgct
     1921 ggctctggca gctgggaccg ggaagatcgg ggtcttggcc tatattctcc agcgggagat
     1981 ccaggagccc gagtgcaggc acctgtccag gaagttcacc gagtgggcct acgggcccgt
     2041 gcactcctcg ctgtacgacc tgtcctgcat cgacacctgc gagaagaact cggtgctgga
     2101 ggtgatcgcc tacagcagca gcgagacccc taatcgccac gacatgctct tggtggagcc
     2161 gctgaaccga ctcctgcagg acaagtggga cagattcgtc aagcgcatct tctacttcaa
     2221 cttcctggtc tactgcctgt acatgatcat cttcaccatg gctgcctact acaggcccgt
     2281 ggatggcttg cctcccttta agatggaaaa aactggagac tatttccgag ttactggaga
     2341 gatcctgtct gtgttaggag gagtctactt ctttttccga gggattcagt atttcctgca
     2401 gaggcggccg tcgatgaaga ccctgtttgt ggacagctac agtgagatgc ttttctttct
     2461 gcagtcactg ttcatgctgg ccaccgtggt gctgtacttc agccacctca aggagtatgt
     2521 ggcttccatg gtattctccc tggccttggg ctggaccaac atgctctact acacccgcgg
     2581 tttccagcag atgggcatct atgccgtcat gatagagaag atgatcctga gagacctgtg
     2641 ccgtttcatg tttgtctaca tcgtcttctt gttcgggttt tccacagcgg tggtgacgct
     2701 gattgaagac gggaagaatg actccctgcc gtctgagtcc acgtcgcaca ggtggcgggg
     2761 gcctgcctgc aggccccccg atagctccta caacagcctg tactccacct gcctggagct
     2821 gttcaagttc accatcggca tgggcgacct ggagttcact gagaactatg acttcaaggc
     2881 tgtcttcatc atcctgctgc tggcctatgt aattctcacc tacatcctcc tgctcaacat
     2941 gctcatcgcc ctcatgggtg agactgtcaa caagatcgca caggagagca agaacatctg
     3001 gaagctgcag agagccatca ccatcctgga cacggagaag agcttcctta agtgcatgag
     3061 gaaggccttc cgctcaggca agctgctgca ggtggggtac acacctgatg gcaaggacga
     3121 ctaccggtgg tgcttcaggg tggacgaggt gaactggacc acctggaaca ccaacgtggg
     3181 catcatcaac gaagacccgg gcaactgtga gggcgtcaag cgcaccctga gcttctccct
     3241 gcggtcaagc agagtttcag gcagacactg gaagaacttt gccctggtcc cccttttaag
     3301 agaggcaagt gctcgagata ggcagtctgc tcagcccgag gaagtttatc tgcgacagtt
     3361 ttcagggtct ctgaagccag aggacgctga ggtcttcaag agtcctgccg cttccgggga
     3421 gaagctcgag tacccatacg acgtacctga ttatgcaact tatccttatg atgtacctga
     3481 ttatgctagc tatccttatg atgtcccaga ctacgcatga gggcccgttt aaacccgctg
     3541 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3601 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3661 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3721 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3781 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4261 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4321 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4381 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4441 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4501 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4561 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4621 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4681 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4741 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4801 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4861 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4921 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4981 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5041 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5101 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5161 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5221 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5281 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5341 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5401 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5461 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5521 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5581 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5641 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5701 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5761 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5881 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7921 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7981 atggt
//
