LOCUS V037529 8893 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037529 VERSION V037529 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 889..2538 /codon_start=1 /note="synthetic luc2 version of the luciferase gene" /product="firefly luciferase" /protein_id="" /transl_table=1 /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" /label="luciferase" CDS 2575..4425 /label="Syna(NM_001013751)" /note="Syna(NM_001013751)" /gene="Syna" polyA_signal 4460..4684 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4730..5158 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5172..5501 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5568..6362 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6536..6657 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6706..6722) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6730..6746 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6754..6784) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6799..6820 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7108..7693) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7864..8724) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8725..8829) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcaca ttccacattc cacattccat ggaagatgcc 901 aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag 961 cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac 1021 gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca 1081 gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat 1141 agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca 1201 gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc 1261 gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg 1321 atcatacaaa agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg 1381 tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag 1441 agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg 1501 cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac 1561 cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac 1621 cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc 1681 atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct 1741 gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac 1801 gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt 1861 gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa 1921 acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag 1981 gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg 2041 aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac 2101 cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc 2161 tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac 2221 aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc 2281 ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc 2341 gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc 2401 caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa 2461 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag 2521 ggcggcaaga tcgccgtggg cggcggaggg agtggaggag ggggaagctc tagaatggtt 2581 cgtccttggg ttttctgtct cctcctgttc ccttgttcct ctgcctactc ggacagctgg 2641 atgcccctgg taaacctcac tcaacacctc ctccaggagg ctaactcttc cttctcttcc 2701 aactgctggg tctgcttatc catccaaacc cagcgctctc tagccatgcc agccccacta 2761 aggacttgga cagagacacc catgaaactt cgaatcatgt actcagcccg gaccctctcc 2821 ggcccatacc ctatcaccga ccttgagagg cgcctccaga atttccaacc attgactccc 2881 cactcctctt ttgtcaaccc tgaccagcgg gccattgctt tccttcagat caccagcgtg 2941 acaggcatac ttcccatact ttctcggatc acctcggtga gataccccga tgaccacgtc 3001 tatgaatctg cccagcgccc catatggggc tcactctcca cccagacgat cctcacctcc 3061 caggcccctc tctgcatatc ccgcttcttc aagaattcaa accatgccac cttcgtgggc 3121 aaactccctg cctctctttg caatcacacc tttcagctct ccccctctgc caaccaccaa 3181 tccatagatc tgtcctccag ctatgcattc gccccattaa tggccatgcc agggtctaaa 3241 tggagaaacc ccttacgctt ttcaggaccc ccttccctga actcagggat gcctcactac 3301 tcctgcccga tagatgacat ccactgccac acctacccca ccaccccctg gaggtcctgt 3361 ccttccttcc cagctagcac ctgctataat ctcaccctat tcgagccgga caattcgagc 3421 caccctatta ccctgtctgt ggacaccaca tacttcaaga ttaaactcca gggacacaaa 3481 gacccctatc cactctttca gtaccagccc ctcatggggg cagccctctc tggacaatat 3541 tcaatctggg aatatgaacc cactgttaag aaaaacggcg gtatcactcc aaatatcttc 3601 tcccaccttg tctccttaac atactccttc tgcctcaact cctctggtgt tttcttcctc 3661 tgtggaaact caacttatgt ctgtctcccg gccaattggt ccggcgtctg tacccttgtc 3721 ttccaatacc cggatattga actccttccc aacaaccaaa ccatatctgt cccgcttttt 3781 gctacagttc cctcctctgt ccccgcttct cgccggaagc gagcccttcc tctccttcct 3841 ctcctcgcgg gcctgggcat tgcttctgcc ctggggttag gcatcgcggg tatcaccacc 3901 tcaactgtgt atttccaaca gctttccaag gctctctcgg acagcctaga tgaaatagcc 3961 acctccatca tcagcctcca agaccaaata gactcgctgg cgggtgtcgt tctccaaaac 4021 cgcagagctc tggacctcat tgtggctgag agggggggca cctgcctctt cctccaggaa 4081 gagtgctgct tctacataaa ccagtccggg gtagtccggc acgcggcaag gaaacttcga 4141 gaaagggcct cggaactcgg cacaagctcg agttcttgga tccagtggct ggggctagga 4201 ccctggctgc cctcttggtt gacttccctc atggctccca ttctctttat cctggtactg 4261 ctggttttca ggccttgtct tcttaactgc ctgactcatt ctgtatcgcg gcgaatgagt 4321 tctttcattc acaccaccac cgaaggacac gtggacaaga tccttctgct tcgagagtcc 4381 cagtacaaga gacttcccca agagcccccg gaggaggatg ccgtctaggg gcccgtttaa 4441 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 4501 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 4561 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 4621 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 4681 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 4741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 4861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 5041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 5101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 5161 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 5221 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 5281 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 5341 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 5401 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 5461 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 5521 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 5581 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 5641 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 5701 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 5761 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 5821 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 5881 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 5941 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 6001 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 6061 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 6121 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 6181 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 6241 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 6301 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 6361 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 6421 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 6481 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 6541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 6601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 6661 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 6721 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 6781 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 6841 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 6901 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 7081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 7321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 7561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 7621 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7981 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 8101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 8161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 8221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc 8881 gatcccctat ggt //