LOCUS V037735 8040 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V037735 VERSION V037735 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 887..2737 /label="SYVN1(NM_172230)" /note="SYVN1(NM_172230)" /gene="SYVN1" CDS 2744..2809 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" CDS 2846..3556 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 3607..3831 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3877..4305 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4319..4648 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4715..5509 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5683..5804 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5853..5869) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5877..5893 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5901..5931) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5946..5967 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6255..6840) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7011..7871) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7872..7976) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcaac ttaaggatcc gccaccatgt tccgcacggc 901 agtgatgatg gcggccagcc tggcgctgac cggggctgtg gtggctcacg cctactacct 961 caaacaccag ttctacccca ctgtggtgta cctgaccaag tccagcccca gcatggcagt 1021 cctgtacatc caggcctttg tccttgtctt ccttctgggc aaggtgatgg gcaaggtgtt 1081 ctttgggcaa ctgagggcag cagagatgga gcaccttctg gaacgttcct ggtacgccgt 1141 cacagagact tgtctggcct tcaccgtttt tcgggatgac ttcagccccc gctttgttgc 1201 actcttcact cttcttctct tcctcaaatg tttccactgg ctggctgagg accgtgtgga 1261 ctttatggaa cgcagcccca acatctcctg gctctttcac tgccgcattg tctctcttat 1321 gttcctcctg ggcatcctgg acttcctctt cgtcagccac gcctatcaca gcatcctgac 1381 ccgtggggcc tctgtgcagc tggtgtttgg ctttgagtat gccatcctga tgacgatggt 1441 gctcaccatc ttcatcaagt atgtgctgca ctccgtggac ctccagagtg agaacccctg 1501 ggacaacaag gctgtgtaca tgctctacac agagctgttt acaggcttca tcaaggttct 1561 gctgtacatg gccttcatga ccatcatgat caaggtgcac accttcccac tctttgccat 1621 ccggcccatg tacctggcca tgagacagtt caagaaagct gtgacagatg ccatcatgtc 1681 tcgccgagcc atccgcaaca tgaacaccct gtatccagat gccaccccag aggagctcca 1741 ggcaatggac aatgtctgca tcatctgccg agaagagatg gtgactggtg ccaagagact 1801 gccctgcaac cacattttcc ataccagctg cctgcgctcc tggttccagc ggcagcagac 1861 ctgccccacc tgccgtatgg atgtccttcg tgcatcgctg ccagcgcagt caccaccacc 1921 cccggagcct gcggatcagg ggccaccccc tgccccccac cccccaccac tcttgcctca 1981 gccccccaac ttcccccagg gcctcctgcc tccttttcct ccaggcatgt tcccactgtg 2041 gccccccatg ggcccctttc cacctgtccc gcctcccccc agctcaggag aggctgtggc 2101 tcctccatcc accagtgcag cagccctttc tcggcccagt ggagcagcta caaccacagc 2161 tgctggcacc agtgctactg ctgcttctgc cacagcatct ggcccaggct ctggctctgc 2221 cccagaggct ggccctgccc ctggtttccc cttccctcct ccctggatgg gtatgcccct 2281 gcctccaccc tttgccttcc ccccaatgcc tgtgccccct gcgggctttg ctgggctgac 2341 cccagaggag ctacgagctc tggagggcca tgagcggcag cacctggagg cccggctgca 2401 gagcctgcgt aacatccaca cactgctgga cgccgccatg ctgcagatca accagtacct 2461 caccgtgctg gcctccttgg ggcccccccg gcctgccact tcagtcaact ccactgagga 2521 gactgccact acagttgttg ctgctgcctc ctccaccagc atccctagct cagaggccac 2581 gaccccaacc ccaggagcct ccccaccagc ccctgaaatg gaaaggcctc cagctcctga 2641 gtcagtgggc acagaggaga tgcctgagga tggagagccc gatgcagcag agctccgccg 2701 gcgccgcctg cagaagctgg agtctcctgt tgcccacctc gaggactaca aagaccatga 2761 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagg aattcggtgg 2821 aggcggatca ggaggcggtg gatctatggt gagcaagggc gaggaggata acatggccat 2881 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt 2941 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa 3001 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta 3061 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt 3121 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt 3181 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac 3241 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc 3301 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct 3361 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt 3421 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga 3481 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga 3541 cgagctgtac aagtaggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag 3601 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 3661 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 3721 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 3781 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 3841 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa 3901 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 3961 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4021 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4081 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4141 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4201 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 4261 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 4321 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 4381 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag 4441 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 4501 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 4561 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg 4621 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg 4681 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4741 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4801 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4861 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg 4921 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 4981 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 5041 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 5101 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 5161 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 5221 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg 5281 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 5341 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 5401 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5461 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 5521 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 5581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct 5701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 5761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg 5821 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt 5881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 5941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6001 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 6061 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 6121 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 6181 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 6241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 6301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 6361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 6421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 6481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 6541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 6601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 6661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 6721 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 6781 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6961 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 7021 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 7081 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 7141 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 7201 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 7261 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 7321 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 7381 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 7441 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 7501 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 7561 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 7621 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 7681 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 7741 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 7801 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 7861 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 7921 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 7981 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt //