LOCUS V024082 6884 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024082 VERSION V024082 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..2374 /label="SYT7(NM_001252065)" /note="SYT7(NM_001252065)" /gene="SYT7" CDS 2381..2446 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 2484..2708 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2754..3182 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3196..3525 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3592..4386 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4560..4681 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4730..4746) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4754..4770 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4778..4808) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4823..4844 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5132..5717) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5888..6748) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6749..6853) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgtaccggg acccggaggc 961 ggccagccca ggggcgccct cgcgcgacgt cctgctggtc tctgccatca tcaccgtcag 1021 ccttagcgtc actgtcgtcc tctgcggcct ctgccactgg tgtcagcgca aactgggcaa 1081 acgctacaag aattccttgg agacggtggg cacgccagac tcagggcgtg ggcgcagtga 1141 gaagaaggct atcaatggca ccctcctgtc gggcgccaaa gtggccgccg cggcggggct 1201 ggcggtggag cgggaaggcc ggctggggga gaagccggca ccggtgccgc cacccggaga 1261 ggacgccttg agaagcggcg gggctgcccc cagcgagccg ggcagcggtg gcaaggcggg 1321 gagaggccgc tggcggacgg tgcagagcca cctggccgca gggaagctca acttgtccaa 1381 gttgcctgca ggagggaagg cggtgaacac agcccccgtg ccaggccaga caccccacga 1441 tgagtccgac cgccggaccg agccacgttc ctccgtctca gacctcgtca actccctcac 1501 cagcgagatg ctcatgctct ccccaggctc cgaggaggat gaggcccacg agggttgcag 1561 ccgagagaac ctgggccgga tccagttcag tgtcggctac aacttccagg agtccacgct 1621 caccgtgaag atcatgaagg cccaggagct gccggccaag gacttcagcg gcaccagcga 1681 ccccttcgtc aagatctacc tgctgcccga caagaagcac aagctggaga ccaaggtgaa 1741 gcggaagaac ctgaaccccc actggaacga gaccttcctc tttgaaggtt ttccctatga 1801 gaaggtggtg cagaggatcc tctacctcca agtcctggac tatgaccgct tcagccgcaa 1861 cgaccccatt ggggaggtgt ccatccccct taacaaggtg gacctgaccc agatgcagac 1921 cttctggaag gatctgaagc catgcagcga tgggagtggg agccgagggg agctgctctt 1981 gtctctctgc tacaacccct ctgccaactc catcatcgtg aacatcatca aagcccggaa 2041 cctcaaagcc atggacatcg ggggcacatc agacccctac gtgaaggtat ggctgatgta 2101 caaggacaag cgggtggaga agaagaagac ggtgacgatg aagaggaacc tgaaccccat 2161 cttcaatgag tccttcgcct tcgatatccc cacggagaag ctgagggaga cgaccatcat 2221 catcactgtc atggacaagg acaagctcag ccgcaatgac gtcatcggca agatctacct 2281 gtcctggaag agcgggccag gggaggtgaa gcactggaag gacatgattg cccgtccccg 2341 gcagcccgtg gcccagtggc accagctgaa ggccctcgag gactacaaag accatgacgg 2401 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtagt gagggcccgt 2461 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 2521 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 2581 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 2641 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 2701 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 2761 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 2821 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 2881 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 2941 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 3001 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 3061 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 3121 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 3181 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3241 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3361 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3421 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 3481 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 3541 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 3601 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 3661 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 3721 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag 3781 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 3841 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 3901 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 3961 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4021 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 4081 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat 4141 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 4201 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4261 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4321 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4381 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac 4441 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 4501 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 4561 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 4621 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 4681 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 4741 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 4801 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 4861 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 4921 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 4981 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 5041 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 5101 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 5161 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 5221 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 5281 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 5341 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 5401 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 5461 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 5521 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 5581 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 5641 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca 5701 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 5761 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 5821 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 5881 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 5941 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 6001 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 6061 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 6121 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 6181 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 6241 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 6301 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 6361 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 6421 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 6481 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 6541 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 6601 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 6661 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 6721 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 6781 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 6841 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc //