LOCUS       V033612                 7749 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033612
VERSION     V033612
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..3264
                     /label="STAT3(NM_139276)"
                     /note="STAT3(NM_139276)"
                     /gene="STAT3"
     CDS             3286..3315
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3344..3568
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3614..4042
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4056..4385
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4452..5246
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5422..5543
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5592..5608)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5616..5632
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5640..5670)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5685..5706
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5994..6582)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6753..7613)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7614..7718)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatggcc
      961 caatggaatc agctacagca gcttgacaca cggtacctgg agcagctcca tcagctctac
     1021 agtgacagct tcccaatgga gctgcggcag tttctggccc cttggattga gagtcaagat
     1081 tgggcatatg cggccagcaa agaatcacat gccactttgg tgtttcataa tctcctggga
     1141 gagattgacc agcagtatag ccgcttcctg caagagtcga atgttctcta tcagcacaat
     1201 ctacgaagaa tcaagcagtt tcttcagagc aggtatcttg agaagccaat ggagattgcc
     1261 cggattgtgg cccggtgcct gtgggaagaa tcacgccttc tacagactgc agccactgcg
     1321 gcccagcaag ggggccaggc caaccacccc acagcagccg tggtgacgga gaagcagcag
     1381 atgctggagc agcaccttca ggatgtccgg aagagagtgc aggatctaga acagaaaatg
     1441 aaagtggtag agaatctcca ggatgacttt gatttcaact ataaaaccct caagagtcaa
     1501 ggagacatgc aagatctgaa tggaaacaac cagtcagtga ccaggcagaa gatgcagcag
     1561 ctggaacaga tgctcactgc gctggaccag atgcggagaa gcatcgtgag tgagctggcg
     1621 gggcttttgt cagcgatgga gtacgtgcag aaaactctca cggacgagga gctggctgac
     1681 tggaagaggc ggcaacagat tgcctgcatt ggaggcccgc ccaacatctg cctagatcgg
     1741 ctagaaaact ggataacgtc attagcagaa tctcaacttc agacccgtca acaaattaag
     1801 aaactggagg agttgcagca aaaagtttcc tacaaagggg accccattgt acagcaccgg
     1861 ccgatgctgg aggagagaat cgtggagctg tttagaaact taatgaaaag tgcctttgtg
     1921 gtggagcggc agccctgcat gcccatgcat cctgaccggc ccctcgtcat caagaccggc
     1981 gtccagttca ctactaaagt caggttgctg gtcaaattcc ctgagttgaa ttatcagctt
     2041 aaaattaaag tgtgcattga caaagactct ggggacgttg cagctctcag aggatcccgg
     2101 aaatttaaca ttctgggcac aaacacaaaa gtgatgaaca tggaagaatc caacaacggc
     2161 agcctctctg cagaattcaa acacttgacc ctgagggagc agagatgtgg gaatgggggc
     2221 cgagccaatt gtgatgcttc cctgattgtg actgaggagc tgcacctgat cacctttgag
     2281 accgaggtgt atcaccaagg cctcaagatt gacctagaga cccactcctt gccagttgtg
     2341 gtgatctcca acatctgtca gatgccaaat gcctgggcgt ccatcctgtg gtacaacatg
     2401 ctgaccaaca atcccaagaa tgtaaacttt tttaccaagc ccccaattgg aacctgggat
     2461 caagtggccg aggtcctgag ctggcagttc tcctccacca ccaagcgagg actgagcatc
     2521 gagcagctga ctacactggc agagaaactc ttgggacctg gtgtgaatta ttcagggtgt
     2581 cagatcacat gggctaaatt ttgcaaagaa aacatggctg gcaagggctt ctccttctgg
     2641 gtctggctgg acaatatcat tgaccttgtg aaaaagtaca tcctggccct ttggaacgaa
     2701 gggtacatca tgggctttat cagtaaggag cgggagcggg ccatcttgag cactaagcct
     2761 ccaggcacct tcctgctaag attcagtgaa agcagcaaag aaggaggcgt cactttcact
     2821 tgggtggaga aggacatcag cggtaagacc cagatccagt ccgtggaacc atacacaaag
     2881 cagcagctga acaacatgtc atttgctgaa atcatcatgg gctataagat catggatgct
     2941 accaatatcc tggtgtctcc actggtctat ctctatcctg acattcccaa ggaggaggca
     3001 ttcggaaagt attgtcggcc agagagccag gagcatcctg aagctgaccc aggtagcgct
     3061 gccccatacc tgaagaccaa gtttatctgt gtgacaccaa cgacctgcag caataccatt
     3121 gacctgccga tgtccccccg cactttagat tcattgatgc agtttggaaa taatggtgaa
     3181 ggtgctgaac cctcagcagg agggcagttt gagtccctca cctttgacat ggagttgacc
     3241 tcggagtgcg ctacctcccc catgctcgag tctagagggc ccttcgaaca aaaactcatc
     3301 tcagaagagg atctgtgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3361 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3421 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3481 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3541 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     3601 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3661 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3721 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3781 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3841 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3901 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3961 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4021 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4081 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4141 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4201 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4261 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4321 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4381 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     4441 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4501 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4561 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4621 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4681 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4741 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4801 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4861 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4921 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4981 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5041 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5101 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5161 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5221 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     5281 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5341 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5401 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     5461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5521 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     5581 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5641 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5701 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5761 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5821 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5881 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5941 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6001 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6061 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     6121 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6181 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6241 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6301 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6361 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6421 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6481 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6541 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6601 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6661 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6721 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6781 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6841 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6901 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6961 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     7021 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     7081 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     7141 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7201 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7261 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7321 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7381 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7441 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7501 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7561 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7621 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7681 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7741 cctgacgtc
//
