LOCUS       V037530                 7778 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037530
VERSION     V037530
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3217
                     /label="STAT3(NM_139276)"
                     /note="STAT3(NM_139276)"
                     /gene="STAT3"
     CDS             3224..3250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3254..3280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3284..3310
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3345..3569
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3615..4043
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4057..4386
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4453..5247
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5421..5542
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5591..5607)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5615..5631
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5639..5669)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5684..5705
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5993..6578)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6749..7609)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7610..7714)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcccaatgga atcagctaca gcagcttgac acacggtacc tggagcagct
      961 ccatcagctc tacagtgaca gcttcccaat ggagctgcgg cagtttctgg ccccttggat
     1021 tgagagtcaa gattgggcat atgcggccag caaagaatca catgccactt tggtgtttca
     1081 taatctcctg ggagagattg accagcagta tagccgcttc ctgcaagagt cgaatgttct
     1141 ctatcagcac aatctacgaa gaatcaagca gtttcttcag agcaggtatc ttgagaagcc
     1201 aatggagatt gcccggattg tggcccggtg cctgtgggaa gaatcacgcc ttctacagac
     1261 tgcagccact gcggcccagc aagggggcca ggccaaccac cccacagcag ccgtggtgac
     1321 ggagaagcag cagatgctgg agcagcacct tcaggatgtc cggaagagag tgcaggatct
     1381 agaacagaaa atgaaagtgg tagagaatct ccaggatgac tttgatttca actataaaac
     1441 cctcaagagt caaggagaca tgcaagatct gaatggaaac aaccagtcag tgaccaggca
     1501 gaagatgcag cagctggaac agatgctcac tgcgctggac cagatgcgga gaagcatcgt
     1561 gagtgagctg gcggggcttt tgtcagcgat ggagtacgtg cagaaaactc tcacggacga
     1621 ggagctggct gactggaaga ggcggcaaca gattgcctgc attggaggcc cgcccaacat
     1681 ctgcctagat cggctagaaa actggataac gtcattagca gaatctcaac ttcagacccg
     1741 tcaacaaatt aagaaactgg aggagttgca gcaaaaagtt tcctacaaag gggaccccat
     1801 tgtacagcac cggccgatgc tggaggagag aatcgtggag ctgtttagaa acttaatgaa
     1861 aagtgccttt gtggtggagc ggcagccctg catgcccatg catcctgacc ggcccctcgt
     1921 catcaagacc ggcgtccagt tcactactaa agtcaggttg ctggtcaaat tccctgagtt
     1981 gaattatcag cttaaaatta aagtgtgcat tgacaaagac tctggggacg ttgcagctct
     2041 cagaggatcc cggaaattta acattctggg cacaaacaca aaagtgatga acatggaaga
     2101 atccaacaac ggcagcctct ctgcagaatt caaacacttg accctgaggg agcagagatg
     2161 tgggaatggg ggccgagcca attgtgatgc ttccctgatt gtgactgagg agctgcacct
     2221 gatcaccttt gagaccgagg tgtatcacca aggcctcaag attgacctag agacccactc
     2281 cttgccagtt gtggtgatct ccaacatctg tcagatgcca aatgcctggg cgtccatcct
     2341 gtggtacaac atgctgacca acaatcccaa gaatgtaaac ttttttacca agcccccaat
     2401 tggaacctgg gatcaagtgg ccgaggtcct gagctggcag ttctcctcca ccaccaagcg
     2461 aggactgagc atcgagcagc tgactacact ggcagagaaa ctcttgggac ctggtgtgaa
     2521 ttattcaggg tgtcagatca catgggctaa attttgcaaa gaaaacatgg ctggcaaggg
     2581 cttctccttc tgggtctggc tggacaatat cattgacctt gtgaaaaagt acatcctggc
     2641 cctttggaac gaagggtaca tcatgggctt tatcagtaag gagcgggagc gggccatctt
     2701 gagcactaag cctccaggca ccttcctgct aagattcagt gaaagcagca aagaaggagg
     2761 cgtcactttc acttgggtgg agaaggacat cagcggtaag acccagatcc agtccgtgga
     2821 accatacaca aagcagcagc tgaacaacat gtcatttgct gaaatcatca tgggctataa
     2881 gatcatggat gctaccaata tcctggtgtc tccactggtc tatctctatc ctgacattcc
     2941 caaggaggag gcattcggaa agtattgtcg gccagagagc caggagcatc ctgaagctga
     3001 cccaggtagc gctgccccat acctgaagac caagtttatc tgtgtgacac caacgacctg
     3061 cagcaatacc attgacctgc cgatgtcccc ccgcacttta gattcattga tgcagtttgg
     3121 aaataatggt gaaggtgctg aaccctcagc aggagggcag tttgagtccc tcacctttga
     3181 catggagttg acctcggagt gcgctacctc ccccatgctc gagtacccat acgacgtacc
     3241 tgattatgca acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc
     3301 agactacgca tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3361 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3421 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3481 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3541 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3601 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3961 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4021 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     4081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4141 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4201 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4261 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4321 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4381 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     4441 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4501 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4561 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4621 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4681 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4741 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4801 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4861 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4921 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4981 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     5041 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     5101 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     5161 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     5221 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     5281 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     5341 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     5401 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     5461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5521 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5581 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5641 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5701 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5761 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     6061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     6121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     6181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     6241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     6301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     6361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     6421 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6721 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6781 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6841 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6901 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6961 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7021 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7081 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7141 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7201 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7261 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7321 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7381 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7441 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7501 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7561 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7621 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7681 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7741 acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
