LOCUS       V041473                 7037 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041473
VERSION     V041473
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..2557
                     /label="SOAT1(NM_003101)"
                     /note="SOAT1(NM_003101)"
                     /gene="SOAT1"
     polyA_signal    2604..2828
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      2874..3302
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3316..3645
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3712..4506
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4680..4801
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4850..4866)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4874..4890
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4898..4928)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4943..4964
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5252..5837)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6008..6868)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6869..6973)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gtgggtgaag agaagatgtc tctaagaaac cggctgtcaa agtccaggga
      961 aaatcctgag gaagatgaag accagagaaa ccctgcaaag gagtccctag agacacctag
     1021 taatggtcga attgacataa aacagttgat agcaaagaag ataaagttga cagcagaggc
     1081 agaggaattg aagccatttt ttatgaagga agttggcagt cactttgatg attttgtgac
     1141 caatctcatt gaaaagtcag catcattaga taatggtggg tgcgctctca caaccttttc
     1201 tgttcttgaa ggagagaaaa acaaccatag agcgaaggat ttgagagcac ctccagaaca
     1261 aggaaagatt tttattgcaa ggcgctctct cttagatgaa ctgcttgaag tggaccacat
     1321 cagaacaata tatcacatgt ttattgccct cctcattctc tttatcctca gcacacttgt
     1381 agtagattac attgatgaag gaaggctggt gcttgagttc agcctcctgt cttatgcttt
     1441 tggcaaattt cctaccgttg tttggacctg gtggatcatg ttcctgtcta cattttcagt
     1501 tccctatttt ctgtttcaac attgggccac tggctatagc aagagttctc atccgctgat
     1561 ccgttctctc ttccatggct ttcttttcat gatcttccag attggagttc taggttttgg
     1621 accaacatat gttgtgttag catatacact gccaccagct tcccggttca tcattatatt
     1681 cgagcagatt cgttttgtaa tgaaggccca ctcatttgtc agagagaacg tgcctcgggt
     1741 actaaattca gctaaggaga aatcaagcac tgttccaata cctacagtca accagtattt
     1801 gtacttctta tttgctccta cccttatcta ccgtgacagc tatcccagga atcccactgt
     1861 aagatggggt tatgtcgcta tgaagtttgc acaggtcttt ggttgctttt tctatgtgta
     1921 ctacatcttt gaaaggcttt gtgccccctt gtttcggaat atcaaacagg agcccttcag
     1981 cgctcgtgtt ctggtcctat gtgtatttaa ctccatcttg ccaggtgtgc tgattctctt
     2041 ccttactttt tttgcctttt tgcactgctg gctcaatgcc tttgctgaga tgttacgctt
     2101 tggtgacagg atgttctata aggattggtg gaactccacg tcatactcca actattatag
     2161 aacctggaat gtggtggtcc atgactggct atattactat gcttacaagg actttctctg
     2221 gtttttctcc aagagattca aatctgctgc catgttagct gtctttgctg tatctgctgt
     2281 agtacacgaa tatgccttgg ctgtttgctt gagctttttc tatcccgtgc tcttcgtgct
     2341 cttcatgttc tttggaatgg ctttcaactt cattgtcaat gatagtcgga aaaagccgat
     2401 ttggaatgtt ctgatgtgga cttctctttt cttgggcaat ggagtcttac tctgctttta
     2461 ttctcaagaa tggtatgcac gtcagcactg tcctctgaaa aatcccacat ttttggatta
     2521 tgtccggcca cgttcctgga cttgtcgtta cgtgttttag ctcgagtcta gagggcccgt
     2581 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2641 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2701 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2761 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2821 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     2881 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2941 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3001 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3061 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3121 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3181 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3241 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3301 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     3661 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3721 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3781 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3841 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3901 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3961 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4021 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4081 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4141 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4201 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     4261 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4321 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4381 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4441 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4501 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4561 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4621 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4801 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5761 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     5821 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5881 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5941 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     6001 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     6061 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     6121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6961 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     7021 tcccgatccc ctatggt
//
