LOCUS       V039604                 9313 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039604
VERSION     V039604
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1642..4803
                     /label="SMARCA1(NM_003069)"
                     /note="SMARCA1(NM_003069)"
                     /gene="SMARCA1"
     polyA_signal    4880..5104
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5150..5578
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5592..5921
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5988..6782
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6956..7077
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7126..7142)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7150..7166
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7174..7204)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7219..7240
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7528..8113)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8284..9144)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9145..9249)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag ggaggcggag gtagtggagg agggggaagc
     1621 ggaggcggag ggagtgaatt catggagcag gacactgccg cagtggcagc caccgtggca
     1681 gccgcggatg cgaccgccac tatcgtggtc atagaggacg agcagcccgg gccgtccacc
     1741 tctcaggagg agggagcggc cgccgcggcc accgaagcca ccgcggccac ggagaagggc
     1801 gagaagaaga aggagaaaaa cgtttcttca tttcaactca aacttgctgc taaagcgcct
     1861 aaatctgaaa aggaaatgga cccagaatat gaagagaaaa tgaaagccga ccgagcaaag
     1921 agatttgaat ttttactgaa gcagacagaa ctttttgcac atttcattca gccttcagca
     1981 cagaaatctc caacatctcc actgaacatg aaattgggac gtccccgaat aaagaaagat
     2041 gaaaagcaga gcttaatttc tgctggagac taccgccata ggcgcacaga gcaagaagaa
     2101 gatgaagagc tactgtctga gagtcggaaa acatctaatg tgtgtattag atttgaggtg
     2161 tcaccttcat atgtgaaagg ggggccactg agagattatc agattcgagg actgaattgg
     2221 ttgatctctt tatatgaaaa tggagtcaat ggcattttgg ctgatgaaat gggccttggg
     2281 aaaactttac aaacaattgc tttgcttggt tacctgaaac actaccgaaa tattcctgga
     2341 cctcacatgg ttttagttcc aaagtctact ttacacaact ggatgaatga atttaaacga
     2401 tgggtcccat ctctccgtgt catttgtttt gtcggagaca aggatgccag agctgctttt
     2461 attcgtgatg aaatgatgcc aggagagtgg gatgtttgcg ttacttctta tgagatggta
     2521 attaaagaaa aatctgtatt caaaaagttt cactggcgat acctggtcat tgatgaagct
     2581 cacagaataa agaatgaaaa atctaagctt tcagagattg ttcgtgagtt caagtcgact
     2641 aaccgcttgc tcctaactgg aacacctttg cagaataacc tgcatgaact gtgggcctta
     2701 ctcaactttt tattgcctga tgtctttaat tctgcagatg actttgattc ttggtttgac
     2761 actaaaaatt gtcttggtga tcaaaaactc gtggaaagac ttcatgcagt tttaaaacca
     2821 tttttgttac gccgtataaa aactgatgta gagaagagtc tgccacctaa aaaggaaata
     2881 aagatttact tggggctgag taagatgcaa cgagaatggt atacaaaaat cctgatgaaa
     2941 gatattgatg ttttaaactc ttctggcaag atggacaaga tgcgactctt aaacattctg
     3001 atgcagcttc gaaagtgttg taatcatcca tatctgtttg atggtgctga acctggtcca
     3061 ccttatacca ctgatgagca tattgtcagc aacagtggta aaatggtagt tctggataaa
     3121 ctattggcca aactcaaaga acagggttca agggttctca ttttcagcca gatgactcgc
     3181 ttgctggata ttttggaaga ttattgcatg tggcgtggtt atgagtattg tcgactggat
     3241 ggacaaaccc cgcatgaaga aagagaggat aaattcctag aagtggaatt tctgggtcaa
     3301 agggaagcaa tagaggcttt taatgctcct aatagtagca aattcatctt tatgctaagt
     3361 accagggctg gaggtctcgg aattaacctg gcaagtgctg atgtggttat actatatgat
     3421 tcagactgga acccacaggt tgatctacaa gctatggatc gagcacatcg tattggtcag
     3481 aagaaaccag tacgtgtatt ccgtctcatc actgacaaca ctgttgaaga gaggattgta
     3541 gaaagagctg agataaaact gagactcgat tcaattgtta tacaacaagg aagactcatt
     3601 gaccaacagt ctaacaagct ggcaaaagag gaaatgttac aaatgatacg gcatggagcc
     3661 acccatgttt ttgcttctaa agagagtgag ttgacagatg aagacattac aactattctg
     3721 gaaagagggg aaaagaagac tgcagagatg aatgaacgcc tgcaaaaaat gggagagtct
     3781 tctctaagaa attttagaat ggacattgaa caaagtttat acaaatttga gggagaagat
     3841 tatagagaaa aacagaagct tggcatggtg gaatggattg aacctcctaa acgagaacgc
     3901 aaagcaaact acgcagtgga tgcctacttt agagaggctt tgcgtgtcag cgagccaaag
     3961 attccaaagg ctccacggcc tccaaaacag ccaaatgttc aggattttca atttttccca
     4021 ccacgcttat ttgagctcct ggaaaaggaa attctttatt atcggaagac aataggctat
     4081 aaggttccaa ggaatcctga tatcccaaat ccagctctgg ctcaaagaga agagcaaaaa
     4141 aagattgatg gagctgaacc tcttacacca gaagagactg aagaaaagga aaaacttctc
     4201 acacaaggtt tcacaaactg gactaaacga gattttaacc agtttattaa agctaatgag
     4261 aaatatggaa gagatgacat tgataacata gctcgagagg tagagggcaa atcccctgag
     4321 gaggtcatgg agtattcagc tgtattttgg gaacgttgca atgaattaca ggacattgag
     4381 aaaattatgg ctcaaattga acgtggagaa gcaagaattc aacgaaggat cagtatcaag
     4441 aaagccctgg atgccaaaat tgcaagatac aaggctccat ttcatcagtt gcgcattcag
     4501 tatggaacca gcaaaggaaa gaactatact gaggaagaag atagattctt gatttgtatg
     4561 ttacacaaaa tgggctttga tagagaaaat gtatatgaag aattaagaca gtgtgtacga
     4621 aatgctcccc agtttagatt tgactggttt atcaagtcta ggactgccat ggaattccag
     4681 agacgctgta acactctgat ttcattgatt gagaaagaaa atatggaaat tgaggaaaga
     4741 gagagagcag aaaagaagaa acgggcaact aaaactccaa tggtaaaatt ttcagcattt
     4801 tcctaaggat cctgatatcc agcacagtgg cggccgctcg agtctagagg gcccgtttaa
     4861 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4921 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4981 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5041 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5101 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5161 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5221 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5281 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5341 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5401 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5461 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5521 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5581 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5641 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5701 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5761 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5821 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     5881 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     5941 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6001 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6061 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6121 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6181 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6241 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6301 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6361 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6421 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6481 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6541 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6601 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6661 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6721 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6781 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6841 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6901 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7081 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7141 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7201 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7261 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7321 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7381 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7441 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7501 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7561 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7621 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7681 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7741 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7801 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7861 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7921 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7981 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8041 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8101 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8161 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8221 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8281 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8341 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8401 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8461 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8521 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8581 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8641 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8701 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8761 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8821 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8881 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8941 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9001 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9061 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9121 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9181 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9241 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     9301 gatcccctat ggt
//
