LOCUS V038796 6410 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V038796 VERSION V038796 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1903 /label="SIX3(NM_005413)" /note="SIX3(NM_005413)" /gene="SIX3" CDS 1910..1936 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1977..2201 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2247..2675 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2689..3018 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3085..3879 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4053..4174 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4223..4239) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4247..4263 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4271..4301) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4316..4337 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4625..5210) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5381..6241) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6242..6346) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gtattccgct cccccctaga cctctattcc tcccacttct tgttgccaaa 961 cttcgccgat tctcaccacc gctccatact tctggcgagt agcggcggcg ggaacggtgc 1021 gggaggcggc ggcggcgcgg gaggcggcag cggcggcggg aacggtgcgg gaggcggcgg 1081 tgctggcgga gcaggcggcg gcggcggcgg cggctccagg gcccccccgg aagagttgtc 1141 catgttccag ctgcccaccc tcaacttctc gccggagcag gtggccagcg tctgtgagac 1201 gctggaggag acgggcgaca tcgagcggct gggccgcttc ctctggtcgc tgcccgtggc 1261 ccccggggcg tgcgaggcca tcaacaaaca cgagtcgatc ctgcgcgcgc gcgccgtggt 1321 cgccttccac acgggcaact tccgcgacct ctaccacatc cttgagaacc acaagttcac 1381 caaggagtct cacggcaagc tgcaggccat gtggctcgag gcgcactacc aggaggccga 1441 gaagctgcgc ggccgcccac tcggcccggt ggacaagtac cgcgtgcgca agaagttccc 1501 gctgccacgc accatctggg acggcgagca gaagacgcat tgcttcaagg agcggactcg 1561 gagcctgttg cgggagtggt acctacagga cccctacccc aaccccagca agaaacgcga 1621 actggcgcag gccaccggcc tcactcccac acaagtaggc aactggttta agaaccggcg 1681 gcagcgcgac cgcgccgcgg cggccaagaa caggctccag caccaggcca ttggaccgag 1741 cggcatgcgc tcgctggccg agcccggctg ccccacgcac ggctcggcag agtcgccgtc 1801 cacggcggcc agcccgacca ccagcgtgtc cagcctgacg gagcgcgcag acaccggcac 1861 ctccatcctc tcggtaacct ccagcgactc ggaatgtgat gtactcgagt acccatacga 1921 cgtaccagat tacgcttagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt 1981 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 2041 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 2101 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 2161 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 2221 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 2281 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 2461 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 2521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 2581 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 2641 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 2701 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 2761 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2821 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 2881 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 2941 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 3001 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa 3061 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 3121 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 3181 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 3241 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 3301 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 3361 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 3421 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 3481 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 3541 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 3601 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 3661 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 3721 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 3781 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 3841 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 3901 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 3961 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 4021 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 4081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 4201 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 4261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 4321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 4381 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 4441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 4501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 4561 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 4621 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 4681 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 4741 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4801 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4861 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4921 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4981 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 5041 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 5101 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 5161 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 5221 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 5281 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 5341 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 5401 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 5461 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 5521 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 5581 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 5641 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5701 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5761 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5821 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5881 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5941 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 6001 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 6061 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 6121 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 6181 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 6241 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 6301 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 6361 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt //