LOCUS V019463 6702 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V019463 VERSION V019463 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 933..2132 /label="SIRT7(NM_016538)" /note="SIRT7(NM_016538)" /gene="SIRT7" CDS 2187..2216 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 2232..2249 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 2299..2523 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2569..2997 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3011..3340 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3407..4201 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4375..4496 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4545..4561) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4569..4585 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4593..4623) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4638..4659 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4947..5535) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5706..6566) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6567..6671) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgaggcca ccatggcagc cgggggtctg agccgctccg 961 agcgcaaagc ggcggagcgg gtccggaggt tgcgggagga gcagcagagg gagcgcctcc 1021 gccaggtgtc gcgcatcctg aggaaggcgg cggcggagcg cagcgccgag gagggccggc 1081 tgctggccga gagcgcggac ctggtaacgg agctgcaggg ccggagccgg cggcgcgagg 1141 gcctgaagcg gcggcaggag gaggtgtgcg acgacccgga ggagctgcgg gggaaggtcc 1201 gggagctggc cagcgccgtc cggaacgcca aatacttggt cgtctacaca ggcgcgggaa 1261 tcagcacggc agcgtctatc ccagactacc ggggccctaa tggagtgtgg acactgcttc 1321 agaaagggag aagcgttagt gctgccgacc tgagcgaggc cgagccaacc ctcacccaca 1381 tgagcatcac ccgtctgcat gagcagaagc tggtgcagca tgtggtgtct cagaactgtg 1441 acgggctcca cctgaggagt gggctgccgc gcacggccat ctccgagctc cacgggaaca 1501 tgtacattga agtctgtacc tcctgcgttc ccaacaggga gtacgtgcgg gtgttcgatg 1561 tgacggagcg cactgccctc cacagacacc agacaggccg gacctgccac aagtgtggga 1621 cccagctgcg ggacaccatt gtgcactttg gggagagggg gacgttgggg cagcctctga 1681 actgggaagc ggcgaccgag gctgccagca gagcagacac catcctgtgt ctagggtcca 1741 gcctgaaggt tctaaagaag tacccacgcc tctggtgcat gaccaagccc cctagccggc 1801 ggccgaagct ttacatcgtg aacctgcagt ggaccccgaa ggatgactgg gctgccctga 1861 agctacatgg gaagtgtgat gacgtcatgc ggctcctcat ggccgagctg ggcttggaga 1921 tccccgccta tagcaggtgg caggatccca ttttctcact ggcgactccc ctgcgtgctg 1981 gtgaagaagg cagccacagt cggaagtcgc tgtgcagaag cagagaggag gccccgcctg 2041 gggaccgggg tgcaccgctt agctcggccc ccatcctagg gggctggttt ggcaggggct 2101 gcacaaaacg cacaaaaagg aagaaagtga cgcgaattcc accacactgg actagtggat 2161 ccgagctcgg taccaagctt gggcccgaac aaaaactcat ctcagaagag gatctgaata 2221 gcgccgtcga ccatcatcat catcatcatt gagtttaaac ggtctccagc ttaagtttaa 2281 accgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 2341 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 2401 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 2461 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 2521 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 2581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 2641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 2701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 2761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 2821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 2881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 2941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 3001 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 3061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 3241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 3301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 3361 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 3421 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 3481 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 3541 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 3601 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 3661 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 3721 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 3781 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 3841 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 3901 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 3961 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4021 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4081 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4141 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4201 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 4261 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 4321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg 4381 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 4441 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 4501 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct 4561 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 4621 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 4681 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 4741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 4801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 4861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 4921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 4981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 5041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 5101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 5161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 5221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 5281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 5341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 5401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 5461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 5521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 5581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 5641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 5701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 5821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 5881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 5941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 6361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc //