LOCUS       V040138                 9287 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040138
VERSION     V040138
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4726
                     /label="SIN3A(NM_001145358)"
                     /note="SIN3A(NM_001145358)"
                     /gene="SIN3A"
     CDS             4733..4759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4763..4789
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4793..4819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4854..5078
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5124..5552
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5566..5895
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5962..6756
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6930..7051
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7100..7116)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7124..7140
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7148..7178)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7193..7214
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7502..8087)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8258..9118)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9119..9223)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aagcggcgtt tggatgacca ggagtcaccg gtgtatgcag cccagcagcg
      961 tcggatccct ggcagcacag aggcttttcc tcaccagcac cgggtgcttg cccctgcccc
     1021 tcctgtgtat gaagcagtgt ctgagaccat gcagtcagct acgggaattc agtactctgt
     1081 aacacccagc taccaggttt cagccatgcc acagagctcc ggcagtcatg ggcccgctat
     1141 agcagcagtt catagcagcc atcatcaccc aacagcggtg cagccccacg gaggccaggt
     1201 ggtccagagt catgctcatc cagccccacc agttgcacca gtgcagggac agcagcaatt
     1261 tcagaggctg aaggtggagg atgcgctatc ttatcttgac caggtgaagc tgcagtttgg
     1321 tagtcagcct caggtctaca atgatttcct tgacatcatg aaggaattta aatctcagag
     1381 catcgacacc ccaggagtga ttagtcgtgt gtcccagcta ttcaaaggcc accccgatct
     1441 gataatggga ttcaacacct tcttgccccc tggctacaaa attgaggtgc aaaccaatga
     1501 catggtgaat gtgacaactc ctggccaggt tcatcagatt cccacccatg gcatccagcc
     1561 acagcctcaa ccaccacccc aacatccttc ccagccttca gcccagtcag ccccagctcc
     1621 tgcccagcca gctcctcagc ccccacctgc caaagtcagc aagccctccc aactgcaagc
     1681 acatactccg gccagtcagc agactccccc acttccaccg tatgcatccc cacgttctcc
     1741 gccggtccag cctcacacac cagtgacaat ctcgttggga acggccccat ccttgcagaa
     1801 caatcaacct gtggagttta atcatgccat caactatgtt aataagatca agaacagatt
     1861 tcagggccaa ccagacatct acaaagcatt cctggagatt ttgcacacat atcagaaaga
     1921 gcagagaaat gccaaggaag ctggaggaaa ctacactcca gcattgacag agcaggaggt
     1981 gtatgcccag gttgctcgtc tctttaaaaa ccaggaagat ttgttgtcag agtttggaca
     2041 attcctacca gatgccaaca gctccgtgct tttaagcaaa acaactgctg agaaggttga
     2101 ttctgtgaga aatgatcatg gaggcactgt caagaagccc caactgaaca acaagccgca
     2161 gaggcccagc cagaatggct gccagatccg cagacatcct acaggaacca cccctccagt
     2221 taagaagaaa cccaaactgc tcaatctgaa ggattcttct atggcagatg ccagcaaaca
     2281 tggtggtgga acagaatcgt tattttttga taaggtccga aaggctcttc ggagtgcaga
     2341 agcctacgaa aatttcctac gctgtcttgt tatttttaac caggaggtga tctctcgtgc
     2401 tgagcttgtg caactagtct ctcctttcct ggggaaattc cctgagttgt ttaattggtt
     2461 taaaaacttt ctgggctata aggagtctgt acatctggaa acttatccaa aggagcgagc
     2521 cacagagggc attgctatgg agatagatta tgcttcttgt aaacgattgg gctccagcta
     2581 tcgagcctta ccaaagagtt accagcagcc caagtgtaca ggacggactc ctctctgtaa
     2641 agaggtttta aatgatacct gggtttcctt cccttcgtgg tctgaggact ctacctttgt
     2701 gagttccaag aagactcaat atgaagaaca tatttatcgt tgtgaagatg aacgctttga
     2761 gcttgatgta gttttagaga ccaatctggc aacaatccgg gttctggaag caatacagaa
     2821 gaagctttcc cgcttgtctg ctgaagaaca agccaaattt cgcttggaca acacccttgg
     2881 gggcacatca gaagtcatcc atagaaaagc actccagagg atatatgctg ataaagcagc
     2941 tgacatcatt gatggtctga gaaagaatcc ctccattgct gttccaattg tccttaaaag
     3001 gttgaagatg aaagaggaag aatggcgaga agctcagaga ggctttaaca aagtatggcg
     3061 agaacaaaat gagaaatact acttgaagtc tctggaccac caggggatca actttaaaca
     3121 gaatgacacc aaggtcctga ggtctaagag cttactcaat gagattgaga gtatctatga
     3181 tgagaggcaa gagcaggcta cggaggagaa tgctggtgta cctgttggcc cacacctctc
     3241 acttgcgtat gaagacaaac aaatactgga agatgctgct gctctgatta tccaccatgt
     3301 gaagaggcag acaggcattc agaaggagga caaatataag ataaaacaaa tcatgcatca
     3361 ttttattcca gatttgctct ttgcccaaag aggtgatctc tcagatgtgg aggaagagga
     3421 agaagaagag atggatgtag atgaagccac aggggcagtt aagaagcaca atggtgttgg
     3481 gggcagtccc cctaagtcca agttactgtt tagtaacaca gcagctcaaa aattaagagg
     3541 aatggatgaa gtatacaacc tcttctatgt caacaacaac tggtatattt ttatgcgact
     3601 gcaccagatt ctctgcctga ggctgctacg gatttgttcc caagccgaac ggcaaattga
     3661 agaagaaaac cgagagagag aatgggaacg ggaagtgctg ggcataaagc gagacaagag
     3721 tgacagccct gccattcagc tacgtctcaa agaacctatg gatgttgatg tagaagatta
     3781 ttacccagct ttcctggaca tggtgcggag cctgctggat ggcaacatag actcatcaca
     3841 gtatgaagat tcactgagag agatgttcac cattcatgcc tacattgcct ttaccatgga
     3901 caaactgatc cagagcattg tcagacagct gcagcatatc gtgagtgatg agatctgtgt
     3961 gcaggtgact gacctttacc tggcagaaaa taataatggg gccaccggag gccagctgaa
     4021 cacacagaac tcaaggagcc tcctggagtc aacgtatcag cggaaagctg agcagctaat
     4081 gtcagatgag aattgcttta agcttatgtt tattcagagc caaggccagg tccagctgac
     4141 tattgagctt ctggacacag aagaggagaa ttcggatgac cctgtggaag cagagcgctg
     4201 gtcagactac gtggagcgat acatgaattc agatactacc tcgcctgagc ttcgtgaaca
     4261 tctagcacag aaaccagtat ttctccccag gaatctacgg cggatccgga agtgtcaacg
     4321 tggtcgagag cagcaggaaa aggaagggaa ggaaggaaac agcaagaaga ccatggagaa
     4381 tgtggatagt ctggataagc tggagtgtag attcaagctg aattcctaca agatggtgta
     4441 tgtgatcaaa tcagaggact atatgtatcg gaggaccgcc ctgctccggg ctcatcagtc
     4501 ccatgagcgt gtaagcaagc gtctacatca gagattccag gcctgggtag ataaatggac
     4561 caaggagcat gtgccccgtg aaatggcagc agagaccagc aagtggctca tgggtgaggg
     4621 gctggagggc ctggtgccct gtaccaccac ctgtgataca gagaccctgc attttgtgag
     4681 cattaacaag tatcgtgtca aatacggcac agtattcaaa gcccctctcg agtacccata
     4741 cgacgtacct gattatgcaa cttatcctta tgatgtacct gattatgcta gctatcctta
     4801 tgatgtccca gactacgcat gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5041 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5101 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5461 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5521 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5641 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5701 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5821 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5881 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     5941 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     6001 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     6061 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     6121 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     6181 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     6241 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     6301 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     6361 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6421 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6481 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6541 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6601 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6661 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6721 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     6781 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6841 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6901 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6961 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7021 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     7081 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
     8041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9241 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggt
//
