LOCUS       V026769                 8104 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026769
VERSION     V026769
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3594
                     /label="SH3RF1(NM_020870)"
                     /note="SH3RF1(NM_020870)"
                     /gene="SH3RF1"
     CDS             3595..3624
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3671..3895
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3941..4369
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4383..4712
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4779..5573
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5747..5868
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5917..5933)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5941..5957
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5965..5995)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6010..6031
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6319..6904)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7075..7935)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7936..8040)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggatgaat cagccttgtt ggatcttttg
      961 gagtgtccgg tgtgtctaga gcgccttgat gcttctgcga aggtcttgcc ttgccagcat
     1021 acgttttgca agcgatgttt gctggggatc gtaggttctc gaaatgaact cagatgtccc
     1081 gagtgcagga ctcttgttgg ctcgggtgtc gaggagcttc ccagtaacat cttgctggtc
     1141 agacttctgg atggcatcaa acagaggcct tggaaacctg gtcctggtgg gggaagtggg
     1201 accaactgca caaatgcatt aaggtctcag agcagcactg tggctaattg tagctcaaaa
     1261 gatctgcaga gctcccaggg cggacagcag cctcgggtgc aatcctggag ccccccagtg
     1321 aggggtatac ctcagttacc atgtgccaaa gcattataca actatgaagg aaaagagcct
     1381 ggagacctta aattcagcaa aggtgacatc atcattttgc gaagacaagt ggatgaaaat
     1441 tggtaccatg gggaagtcaa tggaatccat ggctttttcc ccaccaactt tgtgcagatt
     1501 attaaaccgt tacctcagcc cccacctcag tgcaaagcac tttatgactt tgaagtgaaa
     1561 gacaaggaag cagacaaaga ttgccttcca tttgcaaagg atgatgttct gactgtgatc
     1621 cgaagagtgg atgaaaactg ggctgaagga atgctggcag acaaaatagg aatatttcca
     1681 atttcatatg ttgagtttaa ctcggctgct aagcagctga tagaatggga taagcctcct
     1741 gtgccaggag ttgatgctgg agaatgttcc tcggcagcag cccagagcag cactgcccca
     1801 aagcactccg acaccaagaa gaacaccaaa aagcggcact ccttcacttc cctcactatg
     1861 gccaacaagt cctcccaggc atcccagaac cgccactcca tggagatcag cccccctgtc
     1921 ctcatcagct ccagcaaccc cactgctgct gcacggatca gcgagctgtc tgggctctcc
     1981 tgcagtgccc cttctcaggt tcatataagt accaccgggt taattgtgac cccgccccca
     2041 agcagcccag tgacaactgg cccctcgttt actttcccat cagatgttcc ctaccaagct
     2101 gcccttggaa ctttgaatcc tcctcttcca ccaccccctc tcctggctgc cactgtcctt
     2161 gcctccacac caccaggcgc caccgccgct gctgctgctg ctggaatggg accgaggccc
     2221 atggcaggat ccactgacca gattgcacat ttacggccgc agactcgccc cagtgtgtat
     2281 gttgctatat atccatacac tcctcggaaa gaggatgaac tagagctgag aaaaggggag
     2341 atgtttttag tgtttgagcg ctgccaggat ggctggttca aagggacatc catgcatacc
     2401 agcaagatag gggttttccc tggcaattat gtggcaccag tcacaagggc ggtgacaaat
     2461 gcttcccaag ctaaagtccc tatgtctaca gctggccaga caagtcgggg agtgaccatg
     2521 gtcagtcctt ccacggcagg agggcctgcc cagaagctcc agggaaatgg cgtggctggg
     2581 agtcccagtg ttgtccccgc agctgtggta tcagcagctc acatccagac aagtcctcag
     2641 gctaaggtct tgttgcacat gacggggcaa atgacagtca accaggcccg caatgctgtg
     2701 aggacagttg cagcgcacaa ccaggaacgc cccacggcag cagtgacacc catccaggta
     2761 cagaatgccg ccggcctcag ccctgcatct gtgggcctgt cccatcactc gctggcctcc
     2821 ccacaacctg cgcctctgat gccaggctca gccacgcaca ctgctgccat cagtatcagt
     2881 cgagccagtg cccctctggc ctgtgcagca gctgctccac tgacttcccc aagcatcacc
     2941 agtgcttctc tggaggctga gcccagtggc cggatagtga ccgttctccc tggactcccc
     3001 acatctcctg acagtgcttc atcagcttgt gggaacagtt cagcaaccaa accagacaag
     3061 gatagcaaaa aagaaaaaaa gggtttgttg aagttgcttt ctggcgcctc cactaaacgg
     3121 aagccccgcg tgtctcctcc agcatcgccc accctagaag tggagctggg cagtgcagag
     3181 cttcctctcc agggagcggt ggggcccgaa ctgccaccag gaggtggcca tggcagggca
     3241 ggctcctgcc ctgtggacgg ggacggaccg gtcacgactg cagtggcagg agcagccctg
     3301 gcccaggatg cttttcatag gaaggcaagt tccctggact ccgcagttcc catcgctcca
     3361 cctcctcgcc aggcctgttc ctccctgggt cctgtcttga atgagtctag acctgtcgtt
     3421 tgtgaaaggc acagggtggt ggtttcctat cctcctcaga gtgaggcaga acttgaactt
     3481 aaagaaggag atattgtgtt tgttcataaa aaacgagagg atggctggtt caaaggcaca
     3541 ttacaacgta atgggaaaac tggccttttc ccaggaagct ttgtggaaaa catagagcag
     3601 aagctgatct cagaggagga cctgtagctc gagtctagag ggcccgttta aacccgctga
     3661 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3721 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3781 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3841 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3901 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     3961 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4021 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4081 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4141 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4201 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4261 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4321 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4381 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4441 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4501 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4561 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4621 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     4681 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     4741 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4801 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4861 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4921 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     4981 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5041 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5101 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5161 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5221 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5281 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     5341 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5401 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5461 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5521 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5581 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5641 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5701 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     5761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5881 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5941 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6001 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6061 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6121 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6181 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6241 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6301 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6361 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6421 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6481 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6541 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6601 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6661 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6721 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6781 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6841 caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6901 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6961 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7021 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7081 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7141 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7201 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7261 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7321 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7381 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7441 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7501 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7561 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7621 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7681 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7741 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7801 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7861 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7921 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8041 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8101 tggt
//
