LOCUS       V029437                 8756 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029437
VERSION     V029437
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4243
                     /label="SENP6(NM_015571)"
                     /note="SENP6(NM_015571)"
                     /gene="SENP6"
     polyA_signal    4323..4547
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4593..5021
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5035..5364
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5431..6225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6399..6520
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6569..6585)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6593..6609
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6617..6647)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6662..6683
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6971..7556)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7727..8587)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8588..8692)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcggccggca agagcggcgg tagcgcaggg gagattactt ttctggaagc
      961 tttggctaga tcagagtcta agagagatgg aggttttaaa aataattgga gctttgatca
     1021 tgaagaagaa agtgaaggag atacagataa agatgggaca aatctgctca gtgtggatga
     1081 agatgaggat tctgaaacct caaaaggaaa aaagttaaat cgtcgatctg aaattgttgc
     1141 taatagctct ggtgaattca tcttgaagac atatgtaaga cgaaacaagt ctgaaagttt
     1201 taaaactttg aaaggcaacc caattggact taacatgttg agcaacaata agaaattgag
     1261 tgaaaatacg caaaatacgt cattatgttc tggaactgta gttcatggta gacgttttca
     1321 tcatgctcat gcacagatac cagtagtaaa aacagcagcc caaagcagtc tggaccgaaa
     1381 agaaaggaaa gaatacccac ctcatgtcca aaaagttgaa attaatcctg taaggttaag
     1441 tcggctccaa ggtgttgaac gtataatgaa gaaaacagaa gagtccgaat cacaagtgga
     1501 gcctgaaatt aagaggaaag tacaacagaa acgacactgt agtacctatc agcctactcc
     1561 tcctctatct cctgcttcaa aaaaatgttt aacccattta gaggatttgc aaagaaattg
     1621 cagacaagct attactttga atgagtctac tggaccatta ttaagaacgt caattcatca
     1681 gaattctgga ggacagaagt cacaaaacac aggattaaca accaagaagt tttatggcaa
     1741 caatgtggaa aaggttccaa ttgatattat tgtgaattgt gatgacagta aacacactta
     1801 tttacagact aatggaaaag tcattttacc tggggcaaaa atacccaaaa tcacaaactt
     1861 gaaagaaagg aaaacaagtt tgtcagacct aaatgatcca atcattttgt ccagtgatga
     1921 tgatgatgac aacgacagaa ctaacagaag agaaagcata tctcctcagc ctgctgattc
     1981 agcatgttct tcccctgcac catccactgg aaaagtagaa gcagcgctaa atgaaaatac
     2041 ttgcagagca gagcgtgaac tacgaagcat tccagaagac tcagagttaa atacagttac
     2101 attgccaaga aaagcaagaa tgaaagacca gtttggcaat tctattatca acacacctct
     2161 gaaacgtcgt aaagtgtttt ctcaagaacc tccagatgct ttagctttaa gctgccaaag
     2221 ttcctttgac agtgtcattt taaactgtcg aagtatacga gtaggaacac tcttccggct
     2281 gttaatagag cctgtaattt tttgtttaga ttttatcaag atacagctag acgaaccaga
     2341 ccatgatcct gtagagatta tattaaatac ctctgatcta actaaatgtg aatggtgtaa
     2401 tgtccgaaaa ttacctgtag tgtttcttca agcaattcca gcagtttatc aaaagctgag
     2461 catccaactg caaatgaata aggaggataa agtttggaat gattgtaaag gagtaaataa
     2521 attaacaaat ttagaagaac aatatataat tttaattttt caaaatggcc ttgatcctcc
     2581 ggcaaatatg gtatttgaaa gtatcattaa tgaaattggt ataaagaata acatctccaa
     2641 tttttttgcg aaaattccct ttgaagaagc taatggcaga cttgttgcct gtacaagaac
     2701 ctatgaagag agcatcaaag gaagttgtgg gcaaaaggaa aacaaaatta aaactgtatc
     2761 atttgaatct aaaatacaac ttagaagcaa acaagaattt cagttttttg atgaagaaga
     2821 agaaactgga gaaaaccaca ccatcttcat tggcccagta gaaaagttga tagtatatcc
     2881 accacctcca gctaagggag gcatctctgt taccaatgag gacctgcact gtctaaatga
     2941 aggagaattt ttaaatgatg ttattataga cttttatttg aaatacttgg tgcttgaaaa
     3001 actgaagaag gaagacgctg accgaattca tatattcagt tcttttttct ataaacgcct
     3061 taatcagaga gagaggagaa atcatgaaac aactaatctg tcaatacagc aaaaacggca
     3121 tgggagagta aaaacatgga cccggcacgt agatattttt gagaaggatt ttatttttgt
     3181 accccttaat gaagctgcac actggttttt ggctgttgtt tgtttccccg gtttggaaaa
     3241 accaaagtat gaacctaatc ctcattacca tgaaaatgct gtcatacaga aatgttcaac
     3301 tgtagaggac agttgtattt cttcttcagc cagtgaaatg gagagttgtt cacaaaactc
     3361 ttctgccaag cctgtaatta agaagatgct aaacaaaaaa cattgcatag ctgtaattga
     3421 ttccaatcct gggcaggaag aaagtgaccc tcgttataag agaaacatat gcagtgtaaa
     3481 atacagtgtg aaaaaaataa atcatactgc gagtgaaaat gaagaattca ataaaggaga
     3541 atctacatcc cagaaagttg ctgataggac taaaagtgag aatggcctac agaatgaaag
     3601 tttaagttcc acacatcata cagatggctt aagcaaaatc agactaaact atagcgatga
     3661 atcacctgaa gctggtaaaa tgcttgaaga tgaactcgtc gacttctcag aagatcagga
     3721 taaccaggat gatagcagtg acgatggatt cctcgctgat gacaactgca gttcagaaat
     3781 aggacagtgg catttaaagc ctactatctg taaacaacct tgtatcctac ttatggactc
     3841 actccgaggc ccttctcggt caaatgttgt caaaatttta agagagtatt tagaagtgga
     3901 atgggaagtt aaaaaaggaa gcaaaagaag tttttccaaa gatgttatga agggctctaa
     3961 tccaaaagta ccacagcaaa acaacttcag tgactgtggt gtatatgtat tgcagtatgt
     4021 agagagcttt tttgagaatc caattctcag ttttgaacta cctatgaatt tggcaaactg
     4081 gtttcctcca ccaagaatga gaacaaaaag agaagaaatc cgaaacataa ttctgaagct
     4141 acaggaagat cagagcaaag agaaaagaaa gcataaggac acttactcaa cagaagcacc
     4201 tttaggcgaa ggaacagaac aatatgtcaa tagtatctca gattgagaat tctgcagata
     4261 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4321 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4381 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4441 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4501 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4561 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4621 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4681 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4801 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4921 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5041 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5101 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5161 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5281 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5341 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5401 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     5461 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     5521 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     5581 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     5641 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     5701 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     5761 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     5821 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     5881 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     5941 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6001 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6061 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6121 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6181 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     6241 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     6301 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6361 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6421 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6481 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6541 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6601 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6661 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6721 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7501 gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7561 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7621 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7681 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7741 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7801 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7861 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7921 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7981 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8041 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8101 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8161 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8221 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8281 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8341 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8401 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8461 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8521 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8581 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8641 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8701 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggt
//
