LOCUS       V029468                 7441 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029468
VERSION     V029468
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     regulatory      925..934
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             934..963
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             970..2961
                     /label="SDHA(NM_004168)"
                     /note="SDHA(NM_004168)"
                     /gene="SDHA"
     polyA_signal    3008..3232
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3278..3706
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3720..4049
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4116..4910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5084..5205
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5254..5270)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5278..5294
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5302..5332)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5347..5368
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5656..6241)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6412..7272)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7273..7377)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggagcaga agctgatctc agaggaggac
      961 ctggatatca tgtcgggggt ccggggcctg tcgcggctgc tgagcgctcg gcgcctggcg
     1021 ctggccaagg cgtggccaac agtgttgcaa acaggaaccc gaggttttca cttcactgtt
     1081 gatgggaaca agagggcatc tgctaaagtt tcagattcca tttctgctca gtatccagta
     1141 gtggatcatg aatttgatgc agtggtggta ggcgctggag gggcaggctt gcgagctgca
     1201 tttggccttt ctgaggcagg gtttaataca gcatgtgtta ccaagctgtt tcctaccagg
     1261 tcacacactg ttgcagcaca gggaggaatc aatgctgctc tggggaacat ggaggaggac
     1321 aactggaggt ggcatttcta cgacaccgtg aagggctccg actggctggg ggaccaggat
     1381 gccatccact acatgacgga gcaggccccc gccgccgtgg tcgagctaga aaattatggc
     1441 atgccgttta gcagaactga agatgggaag atttatcagc gtgcatttgg tggacagagc
     1501 ctcaagtttg gaaagggcgg gcaggcccat cggtgctgct gtgtggctga tcggactggc
     1561 cactcgctat tgcacacctt atatggaagg tctctgcgat atgataccag ctattttgtg
     1621 gagtattttg ccttggatct cctgatggag aatggggagt gccgtggtgt catcgcactg
     1681 tgcatagagg acgggtccat ccatcgcata agagcaaaga acactgttgt tgccacagga
     1741 ggctacgggc gcacctactt cagctgcacg tctgcccaca ccagcactgg cgacggcacg
     1801 gccatgatca ccagggcagg ccttccttgc caggacctag agtttgttca gttccaccct
     1861 acaggcatat atggtgctgg ttgtctcatt acggaaggat gtcgtggaga gggaggcatt
     1921 ctcattaaca gtcaaggcga aaggtttatg gagcgatacg cccctgtcgc gaaggacctg
     1981 gcgtctagag atgtggtgtc tcggtccatg actctggaga tccgagaagg aagaggctgt
     2041 ggccctgaga aagatcacgt ctacctgcag ctgcaccacc tacctccaga gcagctggcc
     2101 acgcgcctgc ctggcatttc agagacagcc atgatcttcg ctggcgtgga cgtcacgaag
     2161 gagccgatcc ctgtcctccc caccgtgcat tataacatgg gcggcattcc caccaactac
     2221 aaggggcagg tcctgaggca cgtgaatggc caggatcaga ttgtgcccgg cctgtacgcc
     2281 tgtggggagg ccgcctgtgc ctcggtacat ggtgccaacc gcctcggggc aaactcgctc
     2341 ttggacctgg ttgtctttgg tcgggcatgt gccctgagca tcgaagagtc atgcaggcct
     2401 ggagataaag tccctccaat taaaccaaac gctggggaag aatctgtcat gaatcttgac
     2461 aaattgagat ttgctgatgg aagcataaga acatcggaac tgcgactcag catgcagaag
     2521 tcaatgcaaa atcatgctgc cgtgttccgt gtgggaagcg tgttgcaaga aggttgtggg
     2581 aaaatcagca agctctatgg agacctaaag cacctgaaga cgttcgaccg gggaatggtc
     2641 tggaacacgg acctggtgga gaccctggag ctgcagaacc tgatgctgtg tgcgctgcag
     2701 accatctacg gagcagaggc acggaaggag tcacggggcg cgcatgccag ggaagactac
     2761 aaggtgcgga ttgatgagta cgattactcc aagcccatcc aggggcaaca gaagaagccc
     2821 tttgaggagc actggaggaa gcacaccctg tcctatgtgg acgttggcac tgggaaggtc
     2881 actctggaat atagacccgt gatcgacaaa actttgaacg aggctgactg tgccaccgtc
     2941 ccgccagcca ttcgctccta ctgactcgag tctagagggc ccgtttaaac ccgctgatca
     3001 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3181 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3241 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3541 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3601 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3661 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3961 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4021 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4081 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4141 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4201 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4261 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     4321 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4381 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4441 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4501 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4561 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4621 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     4681 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4741 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     4801 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4861 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4921 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4981 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5041 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     5101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     5221 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     5821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     5881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     5941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6181 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7381 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7441 t
//
