LOCUS       V045816                 9328 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045816
VERSION     V045816
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4767
                     /label="SCAP(NM_012235)"
                     /note="SCAP(NM_012235)"
                     /gene="SCAP"
     CDS             4774..4800
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4804..4830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4834..4860
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4895..5119
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5165..5593
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5607..5936
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6003..6797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6971..7092
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7141..7157)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7165..7181
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7189..7219)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7234..7255
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7543..8128)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8299..9159)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9160..9264)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgaccctga ctgaaaggct gcgtgagaag
      961 atatctcggg ccttctacaa ccatgggctc ctctgtgcat cctatcccat ccccatcatc
     1021 ctcttcacag ggttctgcat cttagcctgc tgctacccac tgctgaaact ccccttgcca
     1081 ggaacaggac ctgtggaatt caccacccct gtgaaggatt actcgccccc acctgtggac
     1141 tctgaccgca aacaaggaga gcctactgag cagcctgagt ggtatgtggg tgccccggtg
     1201 gcttatgtcc agcagatatt tgtgaagtcc tcagtgtttc cctggcacaa gaacctcctg
     1261 gcagtagatg tatttcgttc acctttgtcc cgggcattcc aactggtgga ggagatccgg
     1321 aaccacgtgc tgagagacag ctctgggatc aggagcttgg aggagttgtg tctgcaagtg
     1381 accgacctgc tgccaggcct taggaagctc aggaacctac tccctgagca tggatgcctg
     1441 ctgctgtccc ctgggaactt ctggcagaat gactgggaac gcttccatgc tgatcctgac
     1501 atcattggga ccatccacca gcacgagcct aaaaccctgc agacttcagc cacactcaaa
     1561 gacttgttat ttggtgttcc tgggaagtac agcggggtga gcctctacac caggaagagg
     1621 atggtctcct acaccatcac cctggtcttc cagcactacc atgccaagtt cctgggcagc
     1681 ctgcgtgccc gcctgatgct tctgcacccc agccccaact gcagccttcg ggcggagagc
     1741 ctggtccacg tgcacttcaa ggaggagatt ggtgtcgctg agctcatccc ccttgtgacc
     1801 acctacatca tcttgtttgc ctacatctac ttctccacgc ggaagatcga catggtcaag
     1861 tccaagtggg ggctggccct ggctgccgtg gtcacagtgc tcagctcgct gctcatgtct
     1921 gtgggactct gcacactctt cggcctgacg cccaccctca atggcggcga gattttcccc
     1981 taccttgtgg tggttattgg gttagagaat gtgttggtgc tcaccaagtc tgtggtctca
     2041 accccggtag acctggaggt gaagctgcgg atcgcccaag gcctaagcag cgagagctgg
     2101 tccatcatga agaacatggc cacggagctg ggcatcatcc tcatcggcta cttcacccta
     2161 gtgcccgcca tccaggagtt ctgtctcttt gctgtcgtgg ggctggtgtc tgacttcttc
     2221 cttcagatgc tgtttttcac cactgtcctg tccattgaca ttcgccggat ggagctagca
     2281 gacctgaaca agcgactgcc ccctgaggcc tgcctgccct cagccaagcc agtgggacag
     2341 ccaacgcgct acgagcggca gctggctgtg aggccgtcca caccccacac catcacgttg
     2401 cagccgtctt ccttccgaaa cctgcggctc cccaagaggc tgcgtgttgt ctacttcctg
     2461 gcccgcaccc gcctggcaca gcgcctcatc atggctggca ccgttgtctg gattggcatc
     2521 ctggtataca cagacccagc agggctgcgc aactacctcg ctgcccaggt gacggaacag
     2581 agcccattgg gtgagggagc cctggctccc atgcccgtgc ctagtggcat gctgcccccc
     2641 agccacccgg accctgcctt ctccatcttc ccacctgatg cccctaagct acctgagaac
     2701 cagacgtcgc caggcgagtc acctgagcgt ggaggtccag cagaggttgt ccatgacagc
     2761 ccagtcccag aggtaacctg ggggcctgag gatgaggaac tttggaggaa attgtccttc
     2821 cgccactggc cgacgctctt cagctattac aacatcacac tggccaagag gtacatcagc
     2881 ctgctgcccg tcatcccagt cacgctccgc ctgaacccga gggaggctct ggagggccgg
     2941 caccctcagg acggccgcag tgcctggccc ccaccggggc ccatacctgc tgggcactgg
     3001 gaagcaggac ccaagggccc aggtggggtg caggcccatg gagacgtcac gctgtacaag
     3061 gtggcggcgc tgggcctggc caccggcatc gtcttggtgc tgctgctgct ctgcctctac
     3121 cgcgtgctat gcccgcgcaa ctacgggcag ctgggtggtg ggcccgggcg gcggaggcgc
     3181 ggggagctgc cctgcgacga ctacggctat gcgccacccg agacggagat cgtgccgctt
     3241 gtgctgcgcg gccacctcat ggacatcgag tgcctggcca gcgacggcat gctgctggtg
     3301 agctgctgcc tggcaggcca cgtctgcgtg tgggacgcgc agaccgggga ttgcctaacg
     3361 cgcattccgc gcccaggcag gcagcgccgg gacagtggcg tgggcagcgg gcttgaggct
     3421 caggagagct gggaacgact ttcagatggt gggaaggctg gtccagagga gcctggggac
     3481 agccctcccc tgagacaccg cccccggggc cctccgccgc cttccctctt cggggaccag
     3541 cctgacctca cctgcttaat tgacaccaac ttttcagcgc agcctcggtc ctcacagccc
     3601 actcagcccg agccccggca ccgggcggtc tgtggccgct ctcgggactc cccaggctat
     3661 gacttcagct gcctggtgca gcgggtgtac caggaggagg ggctggcggc cgtctgcaca
     3721 ccagccctgc gcccaccctc gcctgggccg gtgctgtccc aggcccctga ggacgagggt
     3781 ggctcccccg agaaaggctc cccttccctc gcctgggccc ccagtgccga gggttccatc
     3841 tggagcttgg agctgcaggg caacctcatc gtggtggggc ggagcagcgg ccggctggag
     3901 gtgtgggacg ccattgaagg ggtgctgtgc tgcagcagcg aggaggtctc ctcaggcatt
     3961 accgctctgg tgttcttgga caaaaggatt gtggctgcac ggctcaacgg ttcccttgat
     4021 ttcttctcct tggagaccca cactgccctc agccccctgc agtttagagg gaccccaggg
     4081 cggggcagtt cccctgcctc tccagtgtac agcagcagcg acacagtggc ctgtcacctg
     4141 acccacacag tgccctgtgc acaccaaaaa cccatcacag ccctgaaagc cgctgctggg
     4201 cgcttggtga ctgggagcca agaccacaca ctgagagtgt tccgtctgga ggactcgtgc
     4261 tgcctcttca cccttcaggg ccactcaggg gccatcacga ccgtgtacat tgaccagacc
     4321 atggtgctgg ccagtggagg acaagatggg gccatctgcc tgtgggatgt actgactggc
     4381 agccgggtca gccatgtgtt tgctcaccgt ggggatgtca cctcccttac ctgtaccacc
     4441 tcctgtgtca tcagcagtgg cctggatgac ctcatcagca tctgggaccg cagcacaggc
     4501 atcaagttct actccattca gcaggacctg ggctgtggtg caagcttggg tgtcatctca
     4561 gacaacctgc tggtgactgg cggccagggc tgtgtctcct tttgggacct aaactacggg
     4621 gacctgttac agacagtcta cctggggaag aacagtgagg cccagcctgc ccgccagatc
     4681 ctggtgctgg acaacgctgc cattgtctgc aactttggca gtgagctcag cctggtgtat
     4741 gtgccctctg tgctggagaa gctggacctc gagtacccat acgacgtacc tgattatgca
     4801 acttatcctt atgatgtacc tgattatgct agctatcctt atgatgtccc agactacgca
     4861 tgagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4921 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4981 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5041 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5101 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5161 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5221 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5281 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5341 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5401 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5461 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5521 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5581 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5641 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5701 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5761 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5821 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     5881 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5941 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     6001 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     6061 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6121 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6181 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6241 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6301 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6361 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6421 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6481 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6541 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6601 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6661 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6721 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6781 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     6841 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6901 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6961 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7021 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7081 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7141 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7201 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7261 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7321 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7381 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7441 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7501 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7561 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7621 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7681 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7741 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7801 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7861 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7921 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7981 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8041 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     8101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8881 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9301 atcgggagat ctcccgatcc cctatggt
//
