LOCUS       V045502                11309 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045502
VERSION     V045502
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..1639
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1676..6799
                     /label="Rictor(NM_030168)"
                     /note="Rictor(NM_030168)"
                     /gene="Rictor"
     CDS             6806..6871
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    6909..7133
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7179..7607
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7621..7950
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8017..8811
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8985..9106
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9155..9171)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9179..9195
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9203..9233)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9248..9269
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9557..10142)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10313..11173)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11174..11278)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      961 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1021 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1081 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1141 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1201 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1261 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1321 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1381 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1441 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1501 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     1561 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1621 catggacgag ctgtacaagg gcggtggagg cagtggtgga ggcggtagcg aattcatggc
     1681 ggcgatcggc cgcggccgct ctctgaagaa cctccgaata cgagggcgga atgacagcgg
     1741 cgaggagaac gtcccgctcg atctgacccg agaaccttct gataacttga gggagattct
     1801 ccaaaatgtg gccaaattgc aaggagtatc aaatatgaga aagctgggcc atctgaataa
     1861 cttcactaag cttctttgcg atattggcca tagtgaagaa aaactgggct ttaattatga
     1921 agacatcata atttgtttgc gcttagcttt attaaatgag gcaaaagaag taagagcagc
     1981 cgggttgcga gcgctgcgct atctcatcca agattccagt attctccaga aggtgctaaa
     2041 actgaaagtg gactatttaa tagctaggtg cattgacatt cagcagagca acgaggtgga
     2101 gaggacacaa gcccttcgct tagtcagaaa gatgattact gtgaatgctt ccttgtttcc
     2161 tagttctgtg gccaactcat taattgcggt tggaaatgat gggcttcagg aacgagacag
     2221 aatggtccgg gcttgcatcg ccatcatctg tgaactagca cttcagaacc cagaggtggt
     2281 agcccttcga ggaggactaa acaccatact gaaaaatgtg attgattgcc aattaagtcg
     2341 aataaatgag gctctcatta ctacaatttt gcaccttctt aatcatccaa agacccggca
     2401 gtatgtgcga gctgatgtag aattagagcg aattttagca ccctatactg atttccacta
     2461 cagacacagt ccagatacgg ctgaaggaca gctcaaagaa gacagagaag cacgatttct
     2521 agccagtaag atgggaatca ttgcgacatt ccgctcgtgg gcaggtatta ttaatttatg
     2581 taagcctgga aattctggga tacagtctct aattggagta ctttgcatac cgaatatgga
     2641 aataaggcga ggtctgctcg aagtgctcta tgacatcttt cgtctccctc tgcccgttgt
     2701 gacggatgag ttcatagaag ccctgctcag tgtagatcca ggaagattcc aggacagttg
     2761 gaggctttcc gatggctttg tggcagctga ggcaaaaact attcttcccc atcgtgctag
     2821 atccaggcca gacctcatgg acaattattt ggctctgata ctctctgcat ttattcgtaa
     2881 tggactttta gagggcttgg tcgaggtgat aacgaacagt gacgaccaca tctcagtgag
     2941 ggcgaccatc cttttaggag agctcctgca tatggcaaac accatcctgc ctcactcgca
     3001 tagccaccat ctacactgct tgccaaccct catgaacatg gctgcgtcct tcgatattcc
     3061 caaggaaaag aggctgcgag ccagtgcagc cctgaactgt ttgaaccgct tccatgagat
     3121 gaagaagcga ggacctaagc cctacagcct tcatttagat cacatcattc agaaagcaat
     3181 cgcaactcac cacaagcggg atcagtatct tcgagttcag aaagatatat ttgttcttaa
     3241 ggatacagag gaagctcttt taataaacct tagagacagc caagtccttc agcataaaga
     3301 gaatcttgac tgggattgga atctgattgg gaccatcctt aagtggccaa atgtaaatct
     3361 aagaaactat aaagatgagc agttgcacag gtttgtgcgc agacttcttt acttttacaa
     3421 gcccagcagc aaactgtacg ctagtctgga tctggacttg gccaagtcca agcagctcac
     3481 agttgttggt tgtcagttta cagaatttct gctcgagtct gaagaggatg ggcaaggata
     3541 cttagaagat ctcgtgaaag atattgttca gtggctcaat gcttcatctg gagtgaaacc
     3601 tgagagaagc cttcagaata atggcttact gaccaccctt agtcaacact acttcttatt
     3661 tatcggaaca ctctcttgtc atcctcatgg agtcaaaatg ctggaaaagt gcagtgtctt
     3721 tcagtgtctc cttaatcttt gttccttgaa aaaccaagat catctgataa aactcactgt
     3781 ttccagccta gactatagca gagatggatt agccagagtc attctttcaa aaatcctcac
     3841 ggcagccaca gatgcttgca gactgtatgc aacaaaacat ttaagggttt tattgagagc
     3901 taacgttgaa ttcttcaaca attggggaat tgagttacta gtaactcagc tacatgataa
     3961 aaacaaaaca atttcttctg aagcgctgga tattcttgat gaagcttgtg aagacaaggc
     4021 caatcttcat gctcttattc agatgaagcc agccttatcc caccttggag acaagggctt
     4081 gcttctactg ctgagatttc tttccattcc gaaaggattt tcttacctga atgaaagggg
     4141 ttatgttgca aaacagttgg aaaagtggca caaggaatat aattcaaagt atgttgactt
     4201 gattgaggaa caacttaatg aagccctcac tacatacagg aagcctattg atggtgataa
     4261 ctacgttcgt cgcagtaatc aaagactaca gcgacctcat gtctacctgc ccgtacacct
     4321 ttatggacaa ctggtacacc acaaaacagg ctgccattta ctggaagtac agagtatcat
     4381 cacagagctc tgtcacaatg ttcgcactcc agacttggac aaatgggaag acattaagaa
     4441 gctgaaagcc tctctttggg ccttgggaaa tattggctca tcgaattggg ggcttaattt
     4501 gctacaggaa gaaaatgtga tcccagatat actaaaactt gcaaaacagt gtgaggtcct
     4561 ttccatccga gggacctgtg tatatgttct tgggctcata gctaaaacca agcaaggctg
     4621 tgatattttg aaatgtcaca gctgggattc tgtaaggcac agtcgcaagc atctatggcc
     4681 agtggttcca gatgatgtgg agcagctctg caatgagctc tcatctgtcc caagcaccct
     4741 cagcttgaac tctgagtcga ccagctctag acataacagt gagagtgagt ctgcaccatc
     4801 aagcatgttc atgctggagg acgaccgctt cggcagcacc tccaccagca catttttcct
     4861 tgacatcaat gaagatgctg agccagcatt ttatgaccga cctggaccca taaaagataa
     4921 aaattcattt cccttttttg gctccagcaa acttgtaaag aatcgtatct taaattcact
     4981 tactttgcct actaaaaaac accgcagtag cagtgatcca aaaggaggaa aactgtcatc
     5041 tgaaaataag acaagcaacc ggcgaatcag aacacttacg gagcccagtg ttgacttgaa
     5101 tcacagcgaa gacttcacaa gctcctcagc acagaaatca ttacagctag agccttcctt
     5161 cgttgggaat aagcaccttg aagacgcggg cagcactcca agtattggag agaatgacct
     5221 caaattcccc aagagttttg gtaccgagac tcacagagaa aacacaagcc gagaaaggtt
     5281 ggttgtggaa ggctcagcaa gctcccacat caagatccgc agccagagct ttaacacgga
     5341 cactacgacg agtggcatca gctccatgag ctcgagcccc tcccgagaga cagtcgctgt
     5401 cgaccccaca gctatggaca cggactgtgg aagtctgagt accgtggtaa gtactaaaac
     5461 tgttaagacg agccactatt tgacaccaca gtctaaccat ctgtctctct ccaaatcgaa
     5521 ctcagtgtcc ctggtgccac caggttcttc tcacaccctt cctagaagag cccagtccct
     5581 taaagcgccc tccattgcaa caataaaaag tctagcagat tgcaacttca gctacacaag
     5641 ctctagagat gcctttggct atgctacatt gaaaaggtta cagcagcaaa gaatgcaccc
     5701 gtccttgtct cactcggaag ctttggcgtc gccggcaaaa gatgtgcttt ttaccgacac
     5761 catcaccatg aaggccaaca gctttgaatc cagactgacg ccaagcaggt ttatgaaagc
     5821 tttaagttat gcgtcattag ataaagaaga tttactaagt cctattaatc acaataccct
     5881 ccagcgctcc tcctcagtta ggtctatggt gtccagtgct acatatggag gttccgatga
     5941 ctacattgga cttgctcttc cagtagacat caatgacatc ttccagataa aggatgttcc
     6001 ttattttcaa tcaaagcacg tgcctccacc agatgaccga ggtgcaagaa tgttttccca
     6061 tgatggagca ggtctttcat ctggagccgg tggtctcgta aagaactctt ttcacttgct
     6121 gcggcagcag atgagtctca cagaaataat gaactctgtc cattcggacg cctccctgtt
     6181 cttagagagc acagaagaca ctgggctgca ggagcacacg gatgacaatt gcctttactg
     6241 cgtctgcatt gaactcctgg gcttccagcc cagtaaccag ctaagttcca tctgcagtca
     6301 ctccgatctt caagatattc cgtactccga ttggtgcgag cagacgatcc acaacccctt
     6361 agaagtggtt ccctctaagt tctcggggat ttctggctgc agcgatggcg catctcagga
     6421 agaaggctca gccagcagca ccaaaagcac tgagctgtta ctgggtgtta aaaccattcc
     6481 agatgacaca cccatgtgcc ggatcctgct tcgtaaggaa gtgctaagat tagttgtcaa
     6541 tttgagtagt tcagtttcca ccaagtgtca cgagaccggg cttctgacca ttaaagagaa
     6601 gtatcctcaa acatttgatg acatatgcct ttattctgaa gtttctcatc tgttgtcaca
     6661 ctgcacattt agacttcagt gtcggcggtt catacaagag ttatttcagg atgtacagtt
     6721 tttacaaatg catgaagaag cagaggctgt gttggcaatt ccaccaattc agcctatagt
     6781 tgatgagtct gctgaatccc tcgaggacta caaagaccat gacggtgatt ataaagatca
     6841 tgacatcgac tacaaggatg acgatgacaa gtagtgaggg cccgtttaaa cccgctgatc
     6901 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6961 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7021 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7081 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7141 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7201 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7261 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7321 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7381 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7441 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7501 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7561 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     7621 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7681 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     7741 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     7801 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     7861 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7921 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7981 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     8041 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     8101 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8161 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     8221 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     8281 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     8341 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     8401 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     8461 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8521 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     8581 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8641 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8701 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8761 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8821 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8881 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8941 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     9001 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9061 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     9121 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     9181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9961 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10081 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11281 catttccccg aaaagtgcca cctgacgtc
//
