LOCUS V018485 6655 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018485 VERSION V018485 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 874..2142 /label="RRM2B(NM_001172477)" /note="RRM2B(NM_001172477)" /gene="RRM2B" polyA_signal 2222..2446 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2492..2920 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2934..3263 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3330..4124 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4298..4419 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4468..4484) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4492..4508 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4516..4546) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4561..4582 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4870..5455) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5626..6486) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6487..6591) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgcc accatgttgc tgttgcgtct tccccctcac 901 cgcagtcacg ccagcccgtt agattgcaag ttgcaggacc gctgtaggaa atgttattcg 961 ccgcggtcag gacaggcctg tccgcccgcc ctcgccgcag cctggcttcg tcgttgcgag 1021 cgccggggag gccgtccccg gggagggcgg aggaaggagc tgactttggg tttgcgtccc 1081 gctcgctgct ctgccccggg gccagccaag gacgacgctt ggaggcctca ggccgggaga 1141 tcatcttcag acaccaacga aagtgaaata aagtcaaatg aagagccact cctaagaaag 1201 agttctcgcc ggtttgtcat ctttccaatc cagtaccctg atatttggaa aatgtataaa 1261 caggcacagg cttccttctg gacagcagaa gaggtcgact tatcaaagga tctccctcac 1321 tggaacaagc ttaaagcaga tgagaagtac ttcatctctc acatcttagc cttttttgca 1381 gccagtgatg gaattgtaaa tgaaaatttg gtggagcgct ttagtcagga ggtgcaggtt 1441 ccagaggctc gctgtttcta tggctttcaa attctcatcg agaatgttca ctcagagatg 1501 tacagtttgc tgatagacac ttacatcaga gatcccaaga aaagggaatt tttatttaat 1561 gcaattgaaa ccatgcccta tgttaagaaa aaagcagatt gggccttgcg atggatagca 1621 gatagaaaat ctacttttgg ggaaagagtg gtggcctttg ctgctgtaga aggagttttc 1681 ttctcaggat cttttgctgc tatattctgg ctaaagaaga gaggtcttat gccaggactc 1741 actttttcca atgaactcat cagcagagat gaaggacttc actgtgactt tgcttgcctg 1801 atgttccaat acttagtaaa taagccttca gaagaaaggg tcagggagat cattgttgat 1861 gctgtcaaaa ttgagcagga gtttttaaca gaagccttgc cagttggcct cattggaatg 1921 aattgcattt tgatgaaaca gtacattgag tttgtagctg acagattact tgtggaactt 1981 ggattctcaa aggtttttca ggcagaaaat ccttttgatt ttatggaaaa catttcttta 2041 gaaggaaaaa caaatttctt tgagaaacga gtttcagagt atcagcgttt tgcagttatg 2101 gcagaaacca cagataacgt cttcaccttg gatgcagatt tttaagaatt ctgcagatat 2161 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 2221 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 2281 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 2341 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 2401 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2461 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2521 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2581 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2641 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 2701 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 2761 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 2821 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 2881 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 2941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 3001 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3061 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 3121 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 3181 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 3241 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 3301 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3361 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3421 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3481 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3541 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3601 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3661 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 3721 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 3781 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 3841 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 3901 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3961 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 4021 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4081 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4141 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4201 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4261 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 4321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4441 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4501 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4561 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4621 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4681 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4741 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4801 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4861 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4921 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4981 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 5041 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 5101 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 5161 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 5221 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 5281 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 5341 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5401 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 5461 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 5521 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 5581 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 5641 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 5701 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 5761 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 5821 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 5881 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 5941 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 6001 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 6061 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 6121 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 6181 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 6241 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 6301 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 6361 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 6421 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 6481 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 6541 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 6601 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggt //