LOCUS       V020780                 8231 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020780
VERSION     V020780
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             896..3670
                     /label="RIGI(NM_014314)"
                     /note="RIGI(NM_014314)"
                     /gene="RIGI"
     CDS             3677..3703
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3707..3733
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3737..3763
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3798..4022
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4068..4496
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4510..4839
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4906..5700
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5874..5995
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6044..6060)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6068..6084
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6092..6122)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6137..6158
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6446..7031)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7202..8062)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8063..8167)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgac
      901 caccgagcag cgacgcagcc tgcaagcctt ccaggattat atccggaaga ccctggaccc
      961 tacctacatc ctgagctaca tggccccctg gtttagggag gaagaggtgc agtatattca
     1021 ggctgagaaa aacaacaagg gcccaatgga ggctgccaca ctttttctca agttcctgtt
     1081 ggagctccag gaggaaggct ggttccgtgg ctttttggat gccctagacc atgcaggtta
     1141 ttctggactt tatgaagcca ttgaaagttg ggatttcaaa aaaattgaaa agttggagga
     1201 gtatagatta cttttaaaac gtttacaacc agaatttaaa accagaatta tcccaaccga
     1261 tatcatttct gatctgtctg aatgtttaat taatcaggaa tgtgaagaaa ttctacagat
     1321 ttgctctact aaggggatga tggcaggtgc agagaaattg gtggaatgcc ttctcagatc
     1381 agacaaggaa aactggccca aaactttgaa acttgctttg gagaaagaaa ggaacaagtt
     1441 cagtgaactg tggattgtag agaaaggtat aaaagatgtt gaaacagaag atcttgagga
     1501 taagatggaa acttctgaca tacagatttt ctaccaagaa gatccagaat gccagaatct
     1561 tagtgagaat tcatgtccac cttcagaagt gtctgataca aacttgtaca gcccatttaa
     1621 accaagaaat taccaattag agcttgcttt gcctgctatg aaaggaaaaa acacaataat
     1681 atgtgctcct acaggttgtg gaaaaacctt tgtttcactg cttatatgtg aacatcatct
     1741 taaaaaattc ccacaaggac aaaaggggaa agttgtcttt tttgcgaatc agatcccagt
     1801 gtatgaacag cagaaatctg tattctcaaa atactttgaa agacatgggt atagagttac
     1861 aggcatttct ggagcaacag ctgagaatgt cccagtggaa cagattgttg agaacaatga
     1921 catcatcatt ttaactccac agattcttgt gaacaacctt aaaaagggaa cgattccatc
     1981 actatccatc tttactttga tgatatttga tgaatgccac aacactagta aacaacaccc
     2041 gtacaatatg atcatgttta attatctaga tcagaaactt ggaggatctt caggcccact
     2101 gccccaggtc attgggctga ctgcctcggt tggtgttggg gatgccaaaa acacagatga
     2161 agccttggat tatatctgca agctgtgtgc ttctcttgat gcgtcagtga tagcaacagt
     2221 caaacacaat ctggaggaac tggagcaagt tgtttataag ccccagaagt ttttcaggaa
     2281 agtggaatca cggattagcg acaaatttaa atacatcata gctcagctga tgagggacac
     2341 agagagtctg gcaaagagaa tctgcaaaga cctcgaaaac ttatctcaaa ttcaaaatag
     2401 ggaatttgga acacagaaat atgaacaatg gattgttaca gttcagaaag catgcatggt
     2461 gttccagatg ccagacaaag atgaagagag caggatttgt aaagccctgt ttttatacac
     2521 ttcacatttg cggaaatata atgatgccct cattatcagt gagcatgcac gaatgaaaga
     2581 tgctctggat tacttgaaag acttcttcag caatgtccga gcagcaggat tcgatgagat
     2641 tgagcaagat cttactcaga gatttgaaga aaagctgcag gaactagaaa gtgtttccag
     2701 ggatcccagc aatgagaatc ctaaacttga agacctctgc ttcatcttac aagaagagta
     2761 ccacttaaac ccagagacaa taacaattct ctttgtgaaa accagagcac ttgtggacgc
     2821 tttaaaaaat tggattgaag gaaatcctaa actcagtttt ctaaaacctg gcatattgac
     2881 tggacgtggc aaaacaaatc agaacacagg aatgaccctc ccggcacaga agtgtatatt
     2941 ggatgcattc aaagccagtg gagatcacaa tattctgatt gccacctcag ttgctgatga
     3001 aggcattgac attgcacagt gcaatcttgt catcctttat gagtatgtgg gcaatgtcat
     3061 caaaatgatc caaaccagag gcagaggaag agcaagaggt agcaagtgct tccttctgac
     3121 tagtaatgct ggtgtaattg aaaaagaaca aataaacatg tacaaagaaa aaatgatgaa
     3181 tgactctatt ttacgccttc agacatggga cgaagcagta tttagggaaa agattctgca
     3241 tatacagact catgaaaaat tcatcagaga tagtcaagaa aaaccaaaac ctgtacctga
     3301 taaggaaaat aaaaaactgc tctgcagaaa gtgcaaagcc ttggcatgtt acacagctga
     3361 cgtaagagtg atagaggaat gccattacac tgtgcttgga gatgctttta aggaatgctt
     3421 tgtgagtaga ccacatccca agccaaagca gttttcaagt tttgaaaaaa gagcaaagat
     3481 attctgtgcc cgacagaact gcagccatga ctggggaatc catgtgaagt acaagacatt
     3541 tgagattcca gttataaaaa ttgaaagttt tgtggtggag gatattgcaa ctggagttca
     3601 gacactgtac tcgaagtgga aggactttca ttttgagaag ataccatttg atccagcaga
     3661 aatgtccaaa ctcgagtacc catacgacgt acctgattat gcaacttatc cttatgatgt
     3721 acctgattat gctagctatc cttatgatgt cccagactac gcatgagggc ccgtttaaac
     3781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4861 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4921 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4981 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5041 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5101 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5161 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5221 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5281 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5341 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5401 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5461 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5521 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5581 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5641 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5701 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5761 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5821 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6001 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6061 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6121 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6241 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6301 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6361 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6961 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7081 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7141 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7201 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7261 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7321 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7381 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7441 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7501 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7561 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7621 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7681 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7741 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7801 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7861 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7921 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7981 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8041 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8101 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8161 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     8221 tcccctatgg t
//
