LOCUS       V028499                 8200 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028499
VERSION     V028499
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3720
                     /label="RBM10(NM_005676)"
                     /note="RBM10(NM_005676)"
                     /gene="RBM10"
     polyA_signal    3767..3991
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4037..4465
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4479..4808
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4875..5669
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5843..5964
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6013..6029)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6037..6053
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6061..6091)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6106..6127
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6415..7000)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7171..8031)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8032..8136)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggagtatg aaagacgtgg tggtcgtggt
      961 gacaggactg gccgctatgg agccactgac cgctcgcagg atgatggtgg ggagaaccgc
     1021 agccgagacc acgactaccg ggacatggac taccgttcat atcctcgcga gtatggcagc
     1081 caggagggca agcatgacta tgacgactca tctgaggagc agagtgcgga ggattcctac
     1141 gaggcctccc cgggctccga gactcagcgt aggcggcggc ggcggcacag gcacagcccc
     1201 accggcccgc caggcttccc ccgagacggc gactatcggg accaggacta tcggaccgag
     1261 caaggggagg aggaggagga ggaggaggat gaggaggagg aggagaaggc cagtaacatc
     1321 gtcatgctga ggatgctgcc acaggcagcc actgaggatg acatccgtgg ccagctgcag
     1381 tcgcacggcg tgcaagcacg ggaggttcgg ctgatgcgga acaaatcttc aggtcagagc
     1441 cggggcttcg ccttcgtcga gtttagtcac ttgcaggacg ctacacgatg gatggaagcc
     1501 aatcagcact ccctcaacat cctgggccag aaggtgtcga tgcactacag tgaccccaag
     1561 cccaagatca atgaggactg gctgtgcaat aagtgtggcg tccagaactt caaacgccga
     1621 gagaagtgct tcaaatgtgg cgtgcccaag tcagaggcag agcagaagct gcccctcggc
     1681 acgaggctgg atcagcagac actgccactg ggtggccggg agctgagcca gggcctgctt
     1741 cccctgccgc agccctacca ggcccaggga gtcctggcct cccaagccct gtcacagggc
     1801 tcggagccaa gctcagagaa cgccaatgac accatcattt tgcgcaacct gaacccacac
     1861 agcaccatgg attccatcct gggggccctg gcaccctacg cggtgctgtc ctcctccaac
     1921 gtgcgcgtca taaaggacaa gcagacccaa ctgaaccgcg gctttgcctt catccagctc
     1981 tccaccatcg tggaggcagc ccagctgctg cagatcctgc aggccctgca cccaccactc
     2041 actatcgacg gcaagaccat caatgttgag tttgccaagg gttctaagag ggacatggcc
     2101 tccaatgaag gcagtcgcat cagtgctgcc tctgtggcca gcactgccat tgctgcggcc
     2161 cagtgggcca tctcacaggc ctcccaaggt ggggagggta cctgggccac ctccgaggag
     2221 ccgccggtcg actacagcta ctaccaacag gatgagggct atggcaacag ccagggcaca
     2281 gagtcttccc tctatgccca tggctacctc aagggcacca agggccctgg catcactgga
     2341 accaaagggg atcccactgg agcaggtccc gaggcctccc tagagcctgg ggccgactct
     2401 gtgtcgatgc aggctttctc tcgcgcccag cctggtgctg ctcctggcat ctaccaacaa
     2461 tcagccgagg cgagcagtag ccagggcact gctgccaaca gccagtcgta taccatcatg
     2521 tcacccgctg tgctcaaatc tgagctccag agccctaccc atcctagttc tgctctccca
     2581 ccggctacca gccccactgc ccaggaatcc tacagccagt accctgttcc cgacgtctct
     2641 acctaccagt acgatgagac ctccggctac tactatgacc cccagaccgg cctctactat
     2701 gaccccaact cccagtatta ctacaatgct cagagccagc agtacctgta ctgggatggg
     2761 gagaggcgga cctatgttcc cgccctggag cagtcggccg acggacataa ggagacaggg
     2821 gcaccctcga aggagggcaa agagaagaag gagaagcaca agaccaagac agctcaacag
     2881 attgccaagg acatggaacg ctgggcccgc agtctcaaca aacaaaaaga aaacttcaaa
     2941 aatagcttcc agcctatcag ctccctgcga gatgacgaga ggcgggagtc agccactgca
     3001 gatgctggct atgccatcct cgagaagaag ggagcactag ccgagagaca gcacaccagc
     3061 atggatctcc cgaaattggc cagtgacgac cgcccaagcc ctccgcgagg actggtggca
     3121 gcctacagcg gggagagtga cagtgaggag gagcaggagc gtgggggccc tgagcgggag
     3181 gagaagctca ccgactggca gaagctggcc tgtctgctct gccgacgcca gttccccagc
     3241 aaagaggcgc tcatccggca ccagcagctc tcagggctcc acaagcaaaa ccttgagatt
     3301 caccggcgag cccacttgtc agaaaacgag ctagaagcac tagagaagaa tgacatggag
     3361 caaatgaagt accgggaccg tgcagctgaa cgcagagaaa agtatggcat ccccgagccg
     3421 ccagagccca agaggaggaa gtacggcggc atatccacag cctctgtaga cttcgagcag
     3481 cctactcggg acgggctggg cagtgacaac attggcagtc ggatgctgca ggccatgggc
     3541 tggaaagagg gcagcggcct gggccgcaag aagcagggca ttgtaacgcc tatcgaggcc
     3601 caaacacggg tgcggggctc cggcctgggt gcacggggca gctcctacgg ggtcacctca
     3661 accgagtcct acaaggagac actgcacaag acaatggtga cccgcttcaa cgaggcccag
     3721 tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3781 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3841 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3901 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3961 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     4021 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4081 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4141 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4201 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4261 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4321 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4381 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4441 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4501 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4561 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4621 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4681 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4741 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4801 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     4861 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4921 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4981 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     5041 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     5101 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     5161 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     5221 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     5281 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     5341 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5401 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5461 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5521 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5581 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5641 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5701 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5761 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5821 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     5881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5941 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     6001 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6061 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     6961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8161 tgacgtcgac ggatcgggag atctcccgat cccctatggt
//
