LOCUS       V029554                 8111 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029554
VERSION     V029554
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3598
                     /label="RBBP8(NM_002894)"
                     /note="RBBP8(NM_002894)"
                     /gene="RBBP8"
     polyA_signal    3678..3902
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3948..4376
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4390..4719
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4786..5580
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5754..5875
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5924..5940)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5948..5964
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5972..6002)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6017..6038
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6326..6911)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7082..7942)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7943..8047)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg aacatctcgg gaagcagctg tggaagccct aactctgcag atacatctag
      961 tgactttaag gacctttgga caaaactaaa agaatgtcat gatagagaag tacaaggttt
     1021 acaagtaaaa gtaaccaagc taaaacagga acgaatctta gatgcacaaa gactagaaga
     1081 attcttcacc aaaaatcaac agctgaggga acagcagaaa gtccttcatg aaaccattaa
     1141 agttttagaa gatcggttaa gagcaggctt atgtgatcgc tgtgcagtaa ctgaagaaca
     1201 tatgcggaaa aaacagcaag agtttgaaaa tatccggcag cagaatctta aacttattac
     1261 agaacttatg aatgaaagga atactctaca ggaagaaaat aaaaagcttt ctgaacaact
     1321 ccagcagaaa attgagaatg atcaacagca tcaagcagct gagcttgaat gtgaggaaga
     1381 cgttattcca gattcaccga taacagcctt ctcattttct ggcgttaacc ggctacgaag
     1441 aaaggagaac ccccatgtcc gatacataga acaaacacat actaaattgg agcactctgt
     1501 gtgtgcaaat gaaatgagaa aagtttccaa gtcttcaact catccacaac ataatcctaa
     1561 tgaaaatgaa attctagtag ctgacactta tgaccaaagt caatctccaa tggccaaagc
     1621 acatggaaca agcagctata cccctgataa gtcatctttt aatttagcta cagttgttgc
     1681 tgaaacactt ggacttggtg ttcaagaaga atctgaaact caaggtccca tgagccccct
     1741 tggtgatgag ctctaccact gtctggaagg aaatcacaag aaacagcctt ttgaggaatc
     1801 tacaagaaat actgaagata gtttaagatt ttcagattct acttcaaaga ctcctcctca
     1861 agaagaatta cctactcgag tgtcatctcc tgtatttgga gctacctcta gtatcaaaag
     1921 tggtttagat ttgaatacaa gtttgtcccc ttctctttta cagcctggga aaaaaaaaca
     1981 tctgaaaaca ctccctttta gcaacacttg tatatctaga ttagaaaaaa ctagatcaaa
     2041 atctgaagat agtgcccttt tcacacatca cagtcttggg tctgaagtga acaagatcat
     2101 tatccagtca tctaataaac agatacttat aaataaaaat ataagtgaat ccctaggtga
     2161 acagaatagg actgagtacg gtaaagattc taacactgat aaacatttgg agcccctgaa
     2221 atcattggga ggccgaacat ccaaaaggaa gaaaactgag gaagaaagtg aacatgaagt
     2281 aagctgcccc caagcttctt ttgataaaga aaatgctttc ccttttccaa tggataatca
     2341 gttttccatg aatggagact gtgtgatgga taaacctctg gatctgtctg atcgattttc
     2401 agctattcag cgtcaagaga aaagccaagg aagtgagact tctaaaaaca aatttaggca
     2461 agtgactctt tatgaggctt tgaagaccat tccaaagggc ttttcctcaa gccgtaaggc
     2521 ctcagatggc aactgcacgt tgcccaaaga ttccccaggg gagccctgtt cacaggaatg
     2581 catcatcctt cagcccttga ataaatgctc tccagacaat aaaccatcat tacaaataaa
     2641 agaagaaaat gctgtcttta aaattcctct acgtccacgt gaaagtttgg agactgagaa
     2701 tgttttagat gacataaaga gtgctggttc tcatgagcca ataaaaatac aaaccaggtc
     2761 agaccatgga ggatgtgaac ttgcatcagt tcttcagtta aatccatgta gaactggtaa
     2821 aataaagtct ctacaaaaca accaagatgt atcctttgaa aatatccagt ggagtataga
     2881 tccgggagca gacctttctc agtataaaat ggatgttact gtaatagata caaaggatgg
     2941 cagtcagtca aaattaggag gagagacagt ggacatggac tgtacattgg ttagtgaaac
     3001 cgttctctta aaaatgaaga agcaagagca gaagggagaa aaaagttcaa atgaagaaag
     3061 aaaaatgaat gatagcttgg aagatatgtt tgatcggaca acacatgaag agtatgaatc
     3121 ctgtttggca gacagtttct cccaagcagc agatgaagag gaggaattgt ctactgccac
     3181 aaagaaacta cacactcatg gtgataaaca agacaaagtc aagcagaaag cgtttgtgga
     3241 gccgtatttt aaaggtgatg aaagagagac tagcttgcaa aattttcctc atattgaggt
     3301 ggttcggaaa aaagaggaga gaagaaaact gcttgggcac acgtgtaagg aatgtgaaat
     3361 ttattatgca gatatgccag cagaagaaag agaaaagaaa ttggcttcct gctcaagaca
     3421 ccgattccgc tacattccac ccaacacacc agagaatttt tgggaagttg gttttccttc
     3481 cactcagact tgtatggaaa gaggttatat taaggaagat cttgatcctt gtcctcgtcc
     3541 aaaaagacgt cagccttaca acgcaatatt ttctccaaaa ggcaaggagc agaagacata
     3601 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     3661 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3721 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3781 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3841 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3901 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3961 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4021 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4081 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4141 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4201 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4261 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4321 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4621 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4741 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     4801 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     4861 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     4921 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     4981 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5041 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5101 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5161 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5221 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5281 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5341 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5401 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5461 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5521 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5581 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5641 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5701 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     5761 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5821 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5881 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     5941 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6001 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6061 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6121 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6181 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6241 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6301 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6361 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6421 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6481 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6541 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6601 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6661 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6721 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6781 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6841 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6901 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6961 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7021 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7081 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7141 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7201 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7261 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7321 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7381 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7441 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7501 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7561 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7621 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7681 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7741 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7801 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7861 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7921 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7981 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8041 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     8101 tcccctatgg t
//
