LOCUS       V043982                 7282 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043982
VERSION     V043982
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..2721
                     /label="Ptpn6(NM_001077705)"
                     /note="Ptpn6(NM_001077705)"
                     /gene="Ptpn6"
     CDS             2728..2754
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2758..2784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2788..2814
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2849..3073
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3119..3547
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3561..3890
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3957..4751
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4925..5046
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5095..5111)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5119..5135
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5143..5173)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5188..5209
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5497..6082)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6253..7113)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7114..7218)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgttgtccc gcgggtggtt tcaccgggac
      961 ctcagcgggc ctgatgcaga gaccctgctg aagggccggg gagtccctgg gagcttcctg
     1021 gctcggccca gccgcaagaa ccagggtgac ttctccctct cagtcagggt ggatgatcag
     1081 gtgactcata ttcggatcca gaactcaggg gacttctatg acctgtacgg aggggagaag
     1141 tttgcgacgc tgacagagct ggtcgagtat tacacgcagc agcagggcat cctgcaggac
     1201 cgagatggca ccatcatcca ccttaagtac ccactgaact gctcggaccc caccagtgag
     1261 aggtggtacc acggccacat atctggaggg caggcggagt cactgctgca ggccaagggc
     1321 gagccctgga catttcttgt gcgtgagagt ctcagccaac ctggtgattt tgtgctctct
     1381 gtgctcaatg accagcccaa ggctggccca ggttccccgc tcagggtcac tcatatcaag
     1441 gttatgtgtg agggtggacg ctatactgtg ggtggctcag agacgtttga cagcctcaca
     1501 gacctggtgg agcacttcaa gaagacaggg attgaggagg cctcgggtgc ctttgtctac
     1561 ctgcggcagc cttactacgc tactcgggta aacgcagctg acattgagaa tcgggtcttg
     1621 gaactgaaca agaagcagga gtcggaggac acagccaagg ctggcttctg ggaggagttt
     1681 gagagtctac aaaagcagga ggtaaagaat ctacaccaac gtctggaagg gcagcggcca
     1741 gagaacaaga gcaagaaccg ctacaagaac attcttccct ttgaccacag ccgagtgatc
     1801 ctgcagggac gtgacagtaa catcccaggc tctgactaca tcaatgccaa ctacgtgaag
     1861 aaccagctgc taggtccaga tgagaactct aagacctaca tcgccagcca gggctgtctg
     1921 gatgccacag tcaatgactt ctggcagatg gcttggcagg agaacactcg tgtcatcgtc
     1981 atgactacca gagaggtgga gaaaggccgg aacaaatgtg tcccatactg gcccgaggtg
     2041 ggcactcagc gtgtctatgg tctctactct gtgaccaaca gtagggagca tgacacagca
     2101 gaatacaaac tgcgaacatt acagatctcc ccactagaca atggggacct ggttcgggag
     2161 atatggcact accagtacct gagctggcct gaccatgggg ttcccagtga gcctgggggt
     2221 gtcctcagct ttctggatca gatcaaccag cgacaggaaa gtttgcctca tgcagggccc
     2281 atcattgtgc attgcagcgc tggcatcggc cgcacgggca ccatcatcgt cattgatatg
     2341 cttatggaaa gcatctccac caaggggcta gactgtgaca ttgatatcca gaagaccatc
     2401 cagatggtac gagcacagcg ctccggcatg gtgcagaccg aggcccagta caagtttatt
     2461 tacgtggcca ttgcccagtt catcgaaacg accaagaaga aactggagat catacaatcc
     2521 cagaagggcc aggagtcgga gtatgggaat atcacgtacc ctcccgctgt gaggagtgcc
     2581 cacgccaaag cctcgcgtac ttcctccaag cacaaggagg aggtgtacga aaacgtgcat
     2641 agcaagagca agaaggaaga gaaagtaaag aagcagcggt cggcagacaa ggagaagaac
     2701 aaaggttctc tcaagaggaa gctcgagtac ccatacgacg tacctgatta tgcaacttat
     2761 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcatgaggg
     2821 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2881 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2941 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3001 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3061 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3541 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3901 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     3961 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     4021 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     4081 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     4141 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4201 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4261 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4321 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4381 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4441 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4501 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4561 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4621 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4681 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4741 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4801 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4861 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4921 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5041 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     5101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6001 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     6061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6361 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     7261 agatctcccg atcccctatg gt
//
